Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   EV46_RS17620 Genome accession   NZ_CP007744
Coordinates   4028883..4029899 (-) Length   338 a.a.
NCBI ID   WP_011095128.1    Uniprot ID   Q6D122
Organism   Pectobacterium atrosepticum strain JG10-08     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4023883..4034899
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EV46_RS17605 (EV46_17985) nsrR 4024258..4024683 (-) 426 WP_011095125.1 nitric oxide-sensing transcriptional repressor NsrR -
  EV46_RS17610 (EV46_17990) - 4024980..4026551 (+) 1572 WP_011095126.1 methyl-accepting chemotaxis protein -
  EV46_RS17615 (EV46_17995) - 4027082..4028914 (+) 1833 WP_011095127.1 diguanylate cyclase -
  EV46_RS17620 (EV46_18000) pilT 4028883..4029899 (-) 1017 WP_011095128.1 type IV pilus twitching motility protein PilT Machinery gene
  EV46_RS17625 (EV46_18005) - 4029921..4030634 (+) 714 WP_039293482.1 YggS family pyridoxal phosphate-dependent enzyme -
  EV46_RS17630 (EV46_18010) proC 4030732..4031553 (+) 822 WP_039295236.1 pyrroline-5-carboxylate reductase -
  EV46_RS17635 (EV46_18015) - 4031604..4032158 (+) 555 WP_010295003.1 YggT family protein -
  EV46_RS17640 (EV46_18020) yggU 4032155..4032445 (+) 291 WP_005974847.1 DUF167 family protein YggU -
  EV46_RS17645 (EV46_18025) - 4032463..4033056 (+) 594 WP_039293489.1 XTP/dITP diphosphatase -
  EV46_RS17650 (EV46_18030) hemW 4033049..4034188 (+) 1140 WP_039293491.1 radical SAM family heme chaperone HemW -
  EV46_RS22740 - 4034419..4034679 (+) 261 WP_071843040.1 type II toxin-antitoxin system ParD family antitoxin -
  EV46_RS17655 (EV46_18035) - 4034630..4034854 (+) 225 WP_080708297.1 type II toxin-antitoxin system RelE/ParE family toxin -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 36718.01 Da        Isoelectric Point: 6.4221

>NTDB_id=123045 EV46_RS17620 WP_011095128.1 4028883..4029899(-) (pilT) [Pectobacterium atrosepticum strain JG10-08]
MELDEWMARSVKHNASDLHLCSGHPPVLRVDGRLQPENTLPCLTPEQVAQWCDVWLEPTQQEQLRQAGQVDGALMLPDGQ
RLRVNVFRQREGLSAALRIIPSVQPSLDALHAPPVLSTLLEKPNGLILITGATGSGKSTTLAAMVGALNDSSDRHVITLE
DPIEFIHVSRRCLIQQREIGAHSASFAQALRAALREDPDVILLGELRDTETIRLALTAAETGHLVLSTLHTRSASQAVDR
LIDVFPGEEKAYVRSQLSTCLQAVVTQKLLPAAQGGRIALYEVLTATAAVSNLIREGKTHQLPGLIQTGAAAGMQTFEQS
YQQRCRDGLISHSCVLAV

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=123045 EV46_RS17620 WP_011095128.1 4028883..4029899(-) (pilT) [Pectobacterium atrosepticum strain JG10-08]
ATGGAGTTGGATGAGTGGATGGCGCGTAGTGTAAAACATAATGCCTCGGATCTGCACCTTTGTAGCGGGCATCCTCCGGT
GTTGCGTGTGGATGGGCGGCTACAGCCTGAAAATACCTTACCATGTTTAACGCCGGAGCAGGTGGCACAATGGTGCGATG
TCTGGCTAGAACCGACTCAGCAAGAGCAACTGCGGCAGGCAGGGCAGGTGGACGGCGCCTTAATGTTGCCTGACGGACAG
CGCCTGCGGGTAAATGTGTTTCGTCAGCGGGAAGGACTGTCTGCGGCGCTACGCATTATCCCATCTGTTCAACCTTCATT
GGATGCGCTACATGCGCCACCCGTGCTCTCGACGCTGCTGGAGAAACCAAACGGACTGATCCTGATTACCGGCGCGACGG
GCAGCGGTAAATCCACGACGCTAGCGGCGATGGTCGGGGCGTTAAACGACAGCAGCGATCGCCATGTGATTACGCTGGAG
GATCCGATCGAGTTTATTCATGTCAGCCGACGTTGCCTGATTCAACAGCGAGAAATCGGTGCGCACAGCGCTTCTTTTGC
GCAGGCGCTACGGGCGGCATTGAGGGAAGATCCTGATGTCATTCTGCTGGGAGAACTGCGCGATACGGAGACGATTCGGC
TGGCGCTGACAGCAGCGGAAACTGGCCACTTGGTGTTATCAACGCTACATACGCGCAGTGCATCACAGGCAGTCGACCGT
TTGATTGATGTTTTTCCCGGTGAAGAGAAAGCCTATGTTCGTAGCCAGTTATCGACCTGTTTGCAGGCGGTAGTGACGCA
AAAGCTATTGCCCGCAGCGCAAGGCGGTCGAATTGCACTTTATGAAGTGCTGACTGCTACAGCGGCAGTCAGCAACCTGA
TTCGTGAGGGAAAAACGCACCAGCTTCCTGGCCTGATTCAAACCGGAGCCGCTGCGGGAATGCAAACGTTCGAGCAAAGC
TACCAGCAGCGCTGCCGGGATGGGCTTATTTCACACAGTTGCGTCCTTGCCGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q6D122

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.818

97.633

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.818

97.633

0.506
  pilT Neisseria meningitidis 8013

50.602

98.225

0.497
  pilT Neisseria gonorrhoeae MS11

50.301

98.225

0.494
  pilT Acinetobacter baylyi ADP1

48.225

100

0.482
  pilT Legionella pneumophila strain ERS1305867

48.943

97.929

0.479
  pilT Pseudomonas stutzeri DSM 10701

48.943

97.929

0.479
  pilT Legionella pneumophila strain Lp02

48.943

97.929

0.479
  pilT Pseudomonas aeruginosa PAK

48.64

97.929

0.476
  pilT Acinetobacter nosocomialis M2

48.338

97.929

0.473
  pilT Acinetobacter baumannii D1279779

48.338

97.929

0.473
  pilT Acinetobacter baumannii strain A118

48.338

97.929

0.473
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.342

98.521

0.417
  pilU Pseudomonas stutzeri DSM 10701

40.059

99.704

0.399
  pilU Vibrio cholerae strain A1552

38.872

99.704

0.388
  pilU Acinetobacter baylyi ADP1

38.82

95.266

0.37

Multiple sequence alignment