Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   BMMGA3_RS12140 Genome accession   NZ_CP007739
Coordinates   2497418..2498458 (-) Length   346 a.a.
NCBI ID   WP_003347627.1    Uniprot ID   I3ECU7
Organism   Bacillus methanolicus MGA3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2492418..2503458
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BMMGA3_RS12110 (BMMGA3_12570) - 2492435..2493238 (-) 804 WP_003347639.1 hypothetical protein -
  BMMGA3_RS12115 (BMMGA3_12575) - 2493235..2493909 (-) 675 WP_003347637.1 hypothetical protein -
  BMMGA3_RS12120 (BMMGA3_12580) pilM 2493912..2494895 (-) 984 WP_003347635.1 type IV pilus biogenesis protein PilM -
  BMMGA3_RS12125 (BMMGA3_12585) - 2494908..2495660 (-) 753 WP_003347633.1 prepilin peptidase -
  BMMGA3_RS12130 (BMMGA3_12590) - 2495669..2496103 (-) 435 WP_003347631.1 type II secretion system protein -
  BMMGA3_RS12135 (BMMGA3_12595) - 2496211..2497416 (-) 1206 WP_003347629.1 type II secretion system F family protein -
  BMMGA3_RS12140 (BMMGA3_12600) pilT 2497418..2498458 (-) 1041 WP_003347627.1 type IV pilus twitching motility protein PilT Machinery gene
  BMMGA3_RS12145 (BMMGA3_12605) - 2498474..2500135 (-) 1662 WP_003347625.1 GspE/PulE family protein -
  BMMGA3_RS12150 (BMMGA3_12610) - 2500159..2501526 (-) 1368 WP_003347624.1 VanW family protein -
  BMMGA3_RS12155 (BMMGA3_12615) - 2501545..2502330 (-) 786 WP_003347622.1 PRC-barrel domain-containing protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38389.61 Da        Isoelectric Point: 9.2898

>NTDB_id=123010 BMMGA3_RS12140 WP_003347627.1 2497418..2498458(-) (pilT) [Bacillus methanolicus MGA3]
MKNKIDLLLRAGFELKASDIHLTVGVPPVFRIHGELKRYGKDPLAPSDTEGMARAIIPENMWEQFKKKGELDFSYGIPGI
SRFRINAYFQRSCIAMAIRIVPTKIPTLQELELPPVLAKIAEKPQGLVLVTGPTGSGKSTTLAAMINHMNQTMRKHIITL
EDPIEYLHKHGTCIIDQREVGFDTNNFANGLRASLRQDPDVIMVGEMRDLETIQTAITAAETGHLVFGTLHTASAPDTIN
RIIDVFPPSQQAQIRFQLASVLVAIISQRLFLKADKKGRKAATEILLNNSAVANLIRNEKIHQIVNIMQTSRAQGMHTLA
TSIKELVESGQVSAEETMPYLLEKVN

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=123010 BMMGA3_RS12140 WP_003347627.1 2497418..2498458(-) (pilT) [Bacillus methanolicus MGA3]
ATGAAAAACAAAATTGATTTATTGCTTCGTGCCGGTTTTGAATTAAAAGCTTCAGATATTCATCTTACCGTCGGGGTTCC
GCCGGTCTTCCGCATTCATGGCGAATTGAAAAGATATGGAAAGGACCCGCTCGCACCTTCCGATACGGAAGGTATGGCAA
GAGCAATCATTCCGGAAAATATGTGGGAACAATTTAAGAAAAAAGGAGAGCTTGATTTCTCATACGGCATTCCCGGAATT
TCCCGTTTTCGAATCAATGCGTATTTTCAAAGGTCGTGCATTGCCATGGCCATTCGCATCGTTCCGACAAAAATTCCGAC
ACTGCAGGAATTGGAACTGCCGCCTGTCCTTGCAAAAATTGCTGAAAAGCCTCAAGGGCTCGTTCTTGTCACCGGACCGA
CTGGGAGCGGCAAATCGACAACACTTGCAGCGATGATCAATCATATGAATCAAACGATGCGAAAACATATCATTACCCTT
GAAGATCCGATTGAATATTTGCATAAGCATGGGACATGCATTATTGACCAGAGGGAAGTCGGATTTGATACAAATAATTT
TGCAAACGGATTAAGGGCATCGCTCAGACAGGATCCTGATGTCATCATGGTCGGAGAAATGCGTGATCTTGAAACGATTC
AAACTGCTATCACTGCAGCGGAAACAGGGCATCTTGTGTTTGGTACTCTACATACGGCTAGTGCTCCGGACACAATTAAC
AGAATTATCGATGTATTTCCGCCTTCCCAGCAAGCCCAAATAAGATTTCAGCTTGCATCCGTTCTTGTGGCGATCATTTC
TCAACGTTTATTTCTGAAAGCAGACAAAAAAGGACGAAAAGCCGCAACTGAAATTTTACTTAACAATTCAGCAGTTGCAA
ATTTAATCAGAAATGAGAAGATCCACCAAATCGTCAACATCATGCAAACATCAAGAGCCCAAGGAATGCATACGCTTGCA
ACGAGCATAAAAGAGCTTGTTGAGTCGGGCCAAGTGTCTGCTGAAGAAACGATGCCTTATTTATTGGAAAAGGTGAATTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I3ECU7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.648

92.775

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.909

95.376

0.486
  pilT Vibrio cholerae strain A1552

50.909

95.376

0.486
  pilT Neisseria meningitidis 8013

47.59

95.954

0.457
  pilT Neisseria gonorrhoeae MS11

47.289

95.954

0.454
  pilT Acinetobacter baylyi ADP1

46.933

94.22

0.442
  pilT Legionella pneumophila strain Lp02

46.933

94.22

0.442
  pilT Legionella pneumophila strain ERS1305867

46.933

94.22

0.442
  pilT Acinetobacter nosocomialis M2

47.95

91.618

0.439
  pilT Acinetobacter baumannii D1279779

47.95

91.618

0.439
  pilT Acinetobacter baumannii strain A118

47.95

91.618

0.439
  pilT Pseudomonas aeruginosa PAK

47.335

92.197

0.436
  pilT Pseudomonas stutzeri DSM 10701

46.708

92.197

0.431
  pilU Pseudomonas stutzeri DSM 10701

41.088

95.665

0.393
  pilU Vibrio cholerae strain A1552

41.176

93.353

0.384
  pilU Acinetobacter baylyi ADP1

39.877

94.22

0.376

Multiple sequence alignment