Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DI57_RS00670 Genome accession   NZ_CP007546
Coordinates   126772..127752 (+) Length   326 a.a.
NCBI ID   WP_029739224.1    Uniprot ID   -
Organism   Enterobacter asburiae L1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 121772..132752
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DI57_RS00630 (DI57_00640) - 121856..122182 (+) 327 WP_023333354.1 YggL family protein -
  DI57_RS00635 (DI57_00645) - 122239..122955 (+) 717 WP_023309135.1 DUF2884 domain-containing protein -
  DI57_RS00640 (DI57_00650) - 123038..123376 (-) 339 WP_029739228.1 endonuclease domain-containing protein -
  DI57_RS00645 (DI57_00655) hemW 123440..124582 (-) 1143 WP_029739227.1 radical SAM family heme chaperone HemW -
  DI57_RS00650 (DI57_00660) - 124575..125168 (-) 594 WP_029739226.1 XTP/dITP diphosphatase -
  DI57_RS22495 (DI57_00665) yggU 125172..125468 (-) 297 WP_023333349.1 DUF167 family protein YggU -
  DI57_RS00660 (DI57_00670) - 125465..126031 (-) 567 WP_010435467.1 YggT family protein -
  DI57_RS00665 (DI57_00675) - 126053..126754 (-) 702 WP_029739225.1 YggS family pyridoxal phosphate-dependent enzyme -
  DI57_RS00670 (DI57_00680) pilT 126772..127752 (+) 981 WP_029739224.1 type IV pilus twitching motility protein PilT Machinery gene
  DI57_RS00675 (DI57_00685) ruvX 127763..128179 (-) 417 WP_029739223.1 Holliday junction resolvase RuvX -
  DI57_RS00680 (DI57_00690) - 128179..128739 (-) 561 WP_008499752.1 YqgE/AlgH family protein -
  DI57_RS00685 (DI57_00695) gshB 128827..129774 (-) 948 WP_029739222.1 glutathione synthase -
  DI57_RS00690 (DI57_00700) rsmE 129794..130525 (-) 732 WP_023309126.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  DI57_RS00695 (DI57_00705) endA 130577..131284 (-) 708 WP_023333343.1 deoxyribonuclease I -
  DI57_RS00700 (DI57_00710) - 131379..131876 (-) 498 WP_029739221.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35468.48 Da        Isoelectric Point: 6.6934

>NTDB_id=120750 DI57_RS00670 WP_029739224.1 126772..127752(+) (pilT) [Enterobacter asburiae L1]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGTWWAKGQVDFAATVTGGQRLRG
SAFKQMSGISVTLRLLPRSCPQLSSLGAPRAIPELLCNDAGLILVTGATGSGKSTTLAAMVDFLNHHADGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFAEALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQRLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=120750 DI57_RS00670 WP_029739224.1 126772..127752(+) (pilT) [Enterobacter asburiae L1]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGATTAGGCCGGCTTGAACCCGCTCCCTTTCCGCCTCCCGACGTTGGGGCGTTATTAAAAGCATGGCTTAACG
ATGAGCAGCAGGGAACATGGTGGGCAAAGGGGCAGGTGGATTTTGCCGCGACGGTGACGGGAGGCCAGCGTCTGCGCGGC
AGTGCCTTTAAGCAGATGAGCGGTATCTCTGTGACGCTGCGGCTGTTGCCGCGTAGCTGCCCGCAACTCTCTTCGCTGGG
CGCGCCGCGGGCGATCCCGGAACTGTTGTGCAATGACGCCGGGCTGATTCTGGTCACGGGGGCGACCGGCAGCGGCAAAT
CCACTACGCTGGCGGCGATGGTCGATTTTCTCAACCACCATGCTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAGCGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCTGAAGCGCTGCG
CAGTGCCTTACGCCAGGATCCGGACGTTATTCTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGTCTGGCGCTGACGG
CGGCGGAAACCGGACATCTGGTGCTGGCGACGCTGCATACGCGCGGGGCATCGCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAGAGGCTGCTTCC
CGATCTACAGGGCGGGCGCGTCGCGTTGTATGAACTGCTGGTGAACACTGCGGCGGCGGCGAATTTGATTCGCGAAGGGA
AAACGTGGCAACTGCCCGGGATCATTCAAACCGGTCAGCAGGCAGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Acinetobacter baylyi ADP1

48.012

100

0.482
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Acinetobacter nosocomialis M2

48.438

98.16

0.475
  pilT Pseudomonas aeruginosa PAK

47.401

100

0.475
  pilT Neisseria meningitidis 8013

47.256

100

0.475
  pilT Neisseria gonorrhoeae MS11

46.951

100

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.168

98.773

0.426
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment