Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   RRADSPS_RS04290 Genome accession   NZ_CP007514
Coordinates   922550..923698 (-) Length   382 a.a.
NCBI ID   WP_084263710.1    Uniprot ID   -
Organism   Rubrobacter radiotolerans strain RSPS-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 917550..928698
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  RRADSPS_RS16815 (RradSPS_0861) - 918011..918826 (-) 816 WP_051589356.1 calcium-binding protein -
  RRADSPS_RS04280 (RradSPS_0862) - 919149..921311 (+) 2163 WP_156947966.1 DUF4832 domain-containing protein -
  RRADSPS_RS04285 (RradSPS_0863) - 921332..922543 (-) 1212 WP_038680946.1 type II secretion system F family protein -
  RRADSPS_RS04290 (RradSPS_0864) pilT 922550..923698 (-) 1149 WP_084263710.1 type IV pilus twitching motility protein PilT Machinery gene
  RRADSPS_RS04295 (RradSPS_0865) - 923724..925472 (-) 1749 WP_084263711.1 GspE/PulE family protein -
  RRADSPS_RS04300 (RradSPS_0866) - 925789..926865 (+) 1077 WP_051589357.1 type IV pilus twitching motility protein PilT -
  RRADSPS_RS04305 (RradSPS_0867) - 926956..927705 (-) 750 WP_051589358.1 biotin/lipoate A/B protein ligase family protein -

Sequence

Protein


Download         Length: 382 a.a.        Molecular weight: 41985.86 Da        Isoelectric Point: 9.2405

>NTDB_id=120243 RRADSPS_RS04290 WP_084263710.1 922550..923698(-) (pilT) [Rubrobacter radiotolerans strain RSPS-4]
MIESGLNDYLLDAFRLGASDLHLTVGVPPSVRVSGRIRHLEAYPPLTQAKTRDLVYGILTDKQRKRLEEELELDFSYAVP
NLARFRVNVYVQKGALGAALRTIPHEITPIEDLGLPKVVSELADRPRGLVLVTGPTGSGKSTTLAAMIDRINTTREKHIM
SVEDPVEFLHTHKKCLVNQREVGSDTRSFASALKHVLRQDPDVILVGEMRDLETISLAVTAAETGHMVFATLHTQDAPQT
VDRIIDVFPPHQQQQIRAQLANALQGVVTQMLVSRREDRGGGRVVASEVLVATPGVRNLIREGKNHQLYSAMQTGSQYGM
QTMDSSLADLVRRGEVSRENALKGSSNPDEVSRLLGLRGSERLGAPDRNGMQTAGGFSRGRR

Nucleotide


Download         Length: 1149 bp        

>NTDB_id=120243 RRADSPS_RS04290 WP_084263710.1 922550..923698(-) (pilT) [Rubrobacter radiotolerans strain RSPS-4]
ATGATAGAGAGCGGCCTCAACGACTACCTTCTCGACGCCTTCAGGCTCGGGGCGAGCGACCTGCACCTGACGGTCGGGGT
CCCGCCCTCGGTGAGGGTCTCGGGGCGCATAAGGCACCTCGAAGCCTACCCCCCGCTCACGCAGGCAAAGACGCGGGACC
TCGTCTACGGCATCCTCACCGACAAGCAGCGAAAGCGCCTCGAAGAAGAGCTAGAGCTCGACTTCTCCTACGCCGTGCCG
AACCTGGCGCGCTTCCGGGTGAACGTCTACGTGCAGAAGGGCGCGCTCGGGGCGGCGCTCCGGACGATTCCGCACGAGAT
CACCCCCATCGAGGACCTCGGGCTCCCGAAGGTCGTCTCCGAGCTTGCCGACCGTCCGAGGGGCCTCGTGCTCGTAACGG
GCCCCACCGGAAGCGGCAAGTCAACGACGCTTGCGGCCATGATCGACCGCATCAACACCACGCGCGAAAAGCACATAATG
AGCGTCGAGGACCCGGTTGAGTTCCTGCACACGCACAAGAAGTGTCTCGTAAACCAGCGCGAGGTCGGCTCGGACACGCG
CAGCTTCGCGAGCGCGCTCAAGCACGTCCTGAGGCAGGACCCGGACGTGATCCTTGTCGGAGAGATGCGCGACCTTGAGA
CGATCTCCCTCGCCGTCACCGCCGCCGAGACGGGACACATGGTCTTTGCGACGCTCCACACCCAGGACGCGCCGCAGACG
GTGGACCGCATAATCGACGTCTTCCCTCCGCACCAGCAGCAGCAGATAAGGGCGCAGCTAGCGAACGCGCTTCAGGGCGT
CGTAACGCAGATGCTCGTTTCGAGACGCGAGGACCGGGGCGGCGGCCGGGTCGTGGCGAGCGAGGTGCTGGTGGCGACGC
CCGGGGTGAGGAACCTTATCCGCGAGGGCAAGAACCACCAGCTCTACTCGGCGATGCAGACGGGCTCGCAGTACGGGATG
CAGACAATGGACTCCTCGCTCGCAGATCTCGTGCGGCGGGGCGAAGTCTCCCGGGAGAACGCCCTCAAGGGCTCTTCTAA
CCCGGACGAGGTCAGCCGCCTTCTCGGCCTTCGCGGCTCGGAGCGGCTGGGCGCGCCGGACAGAAACGGCATGCAGACGG
CGGGCGGCTTCTCCCGAGGACGAAGGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.868

91.361

0.492
  pilT Acinetobacter baumannii D1279779

54.07

90.052

0.487
  pilT Acinetobacter nosocomialis M2

54.07

90.052

0.487
  pilT Acinetobacter baumannii strain A118

54.07

90.052

0.487
  pilT Pseudomonas aeruginosa PAK

55.556

87.173

0.484
  pilT Acinetobacter baylyi ADP1

54.819

86.911

0.476
  pilT Pseudomonas stutzeri DSM 10701

54.354

87.173

0.474
  pilT Legionella pneumophila strain ERS1305867

54.242

86.387

0.469
  pilT Legionella pneumophila strain Lp02

54.242

86.387

0.469
  pilT Neisseria meningitidis 8013

49.856

90.838

0.453
  pilT Neisseria gonorrhoeae MS11

49.856

90.838

0.453
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.75

83.77

0.45
  pilT Vibrio cholerae strain A1552

53.75

83.77

0.45
  pilU Pseudomonas stutzeri DSM 10701

44.828

91.099

0.408
  pilU Vibrio cholerae strain A1552

41.111

94.241

0.387
  pilU Acinetobacter baylyi ADP1

39.837

96.597

0.385

Multiple sequence alignment