Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CL52_RS18745 Genome accession   NZ_CP007511
Coordinates   4039603..4040637 (-) Length   344 a.a.
NCBI ID   WP_043222415.1    Uniprot ID   A0A8D3Y4H8
Organism   Stutzerimonas balearica DSM 6083 strain DSM6083 (=SP1402)     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4034603..4045637
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CL52_RS18725 (CL52_19030) - 4035822..4036223 (-) 402 WP_041108393.1 TM2 domain-containing protein -
  CL52_RS18730 (CL52_19035) - 4036320..4037531 (+) 1212 WP_043222410.1 putative DNA modification/repair radical SAM protein -
  CL52_RS18735 (CL52_19040) - 4037531..4038415 (+) 885 WP_043222413.1 TIGR03915 family putative DNA repair protein -
  CL52_RS18740 (CL52_19045) pilU 4038381..4039526 (-) 1146 WP_041108398.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CL52_RS18745 (CL52_19050) pilT 4039603..4040637 (-) 1035 WP_043222415.1 type IV pilus twitching motility protein PilT Machinery gene
  CL52_RS18750 (CL52_19055) - 4040703..4041392 (+) 690 WP_041108402.1 YggS family pyridoxal phosphate-dependent enzyme -
  CL52_RS18755 (CL52_19060) proC 4041412..4042245 (+) 834 WP_041108404.1 pyrroline-5-carboxylate reductase -
  CL52_RS18760 (CL52_19065) - 4042255..4042845 (+) 591 WP_043222418.1 YggT family protein -
  CL52_RS18765 (CL52_19070) - 4043024..4045000 (+) 1977 WP_052264597.1 dynamin-like GTPase family protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37907.79 Da        Isoelectric Point: 6.8361

>NTDB_id=120186 CL52_RS18745 WP_043222415.1 4039603..4040637(-) (pilT) [Stutzerimonas balearica DSM 6083 strain DSM6083 (=SP1402)]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPAMDHKQVHALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKITDVPRGLVLVTGPTGSGKSTTLAAMLDYLNNTKYHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKAMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSAIQTGGALGMQTLDMCL
KGLVAKGLISRESAREKAKTPENI

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=120186 CL52_RS18745 WP_043222415.1 4039603..4040637(-) (pilT) [Stutzerimonas balearica DSM 6083 strain DSM6083 (=SP1402)]
ATGGATATCACTGAGCTGCTGGCGTTCAGCGCCAAGCAGGGTGCATCGGACCTGCACCTTTCCGCCGGCCTGCCGCCGAT
GATTCGCGTCGACGGCGACGTGCGCAGGATCAACCTGCCGGCGATGGACCACAAGCAGGTGCATGCGCTGATCTACGACA
TTATGAACGACAAGCAGCGCAAGGATTTCGAGGAGTTTCTCGAAACCGACTTCTCGTTCGAGGTCCCCGGCGTGGCGCGT
TTCCGGGTCAACGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTATTCCGGACCATTCCGTCCAAGGTGCTGACCATGGA
AGACCTGGGAATGGGCGAGGTGTTCCGCAAGATCACCGACGTGCCGCGTGGCCTGGTGCTGGTTACCGGCCCGACCGGCT
CGGGTAAGTCGACCACCTTGGCGGCGATGCTCGACTACCTCAACAACACCAAGTACCACCATATCCTCACCATCGAAGAC
CCGATCGAATTCGTCCATGAGTCGAAGAAGTGTCTGGTCAACCAGCGCGAGGTCCATCGCGACACCCTGGGTTTTTCCGA
GGCGCTGCGTTCCGCACTGCGTGAGGACCCGGACATCATCCTCGTCGGCGAGATGCGCGATCTGGAAACCATCCGGCTGG
CGCTGACAGCCGCCGAAACCGGACACCTGGTATTCGGGACCCTGCACACCACCTCGGCGGCCAAGACCATCGACCGTGTG
GTCGACGTGTTCCCGGCCGAGGAAAAGGCCATGGTCCGCTCGATGCTCTCCGAATCGCTGCAGGCAGTGATTTCGCAGAC
ACTGCTGAAGAAGATCGGAGGCGGTCGGGTTGCGGCTCACGAGATCATGATCGGCACCCCGGCGATTCGTAATCTGATCC
GCGAAGACAAGGTGGCGCAGATGTATTCGGCCATCCAGACCGGCGGTGCGCTGGGCATGCAGACGCTCGACATGTGCCTC
AAGGGGCTGGTCGCCAAGGGCCTGATCAGCCGCGAGAGCGCCCGCGAGAAAGCCAAGACCCCGGAAAATATCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

96.802

100

0.968
  pilT Pseudomonas aeruginosa PAK

95.93

100

0.959
  pilT Acinetobacter baumannii D1279779

84.593

100

0.846
  pilT Acinetobacter baumannii strain A118

84.593

100

0.846
  pilT Acinetobacter nosocomialis M2

84.302

100

0.843
  pilT Acinetobacter baylyi ADP1

83.14

100

0.831
  pilT Legionella pneumophila strain Lp02

74.556

98.256

0.733
  pilT Legionella pneumophila strain ERS1305867

74.556

98.256

0.733
  pilT Neisseria meningitidis 8013

68.116

100

0.683
  pilT Neisseria gonorrhoeae MS11

67.826

100

0.68
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.139

97.965

0.677
  pilT Vibrio cholerae strain A1552

69.139

97.965

0.677
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.478

99.709

0.523
  pilU Vibrio cholerae strain A1552

40.58

100

0.407
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Acinetobacter baylyi ADP1

39.306

100

0.395

Multiple sequence alignment