Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACOQPG_RS15965 Genome accession   NZ_OZ244907
Coordinates   3718775..3719863 (+) Length   362 a.a.
NCBI ID   WP_420615937.1    Uniprot ID   -
Organism   MAG: Candidatus Palauibacter sp. isolate c5de0401-b7dc-4d82-98b5-efbda7d568cc     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3713775..3724863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOQPG_RS15940 - 3713850..3714512 (-) 663 WP_420615932.1 hypothetical protein -
  ACOQPG_RS15945 tdh 3714526..3715557 (-) 1032 WP_420615933.1 L-threonine 3-dehydrogenase -
  ACOQPG_RS15950 - 3715826..3716428 (+) 603 WP_420615934.1 energy transducer TonB family protein -
  ACOQPG_RS15955 - 3716564..3717241 (+) 678 WP_420615935.1 ComEA family DNA-binding protein -
  ACOQPG_RS15960 - 3717238..3718764 (+) 1527 WP_420615936.1 GspE/PulE family protein -
  ACOQPG_RS15965 pilT 3718775..3719863 (+) 1089 WP_420615937.1 type IV pilus twitching motility protein PilT Machinery gene
  ACOQPG_RS15970 - 3719970..3721067 (+) 1098 WP_420615938.1 type II secretion system F family protein -
  ACOQPG_RS15975 - 3721141..3721602 (+) 462 WP_420615939.1 hypothetical protein -
  ACOQPG_RS15980 - 3721711..3722136 (+) 426 WP_420615940.1 type IV pilin protein -
  ACOQPG_RS15985 - 3722172..3722699 (+) 528 WP_420615941.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACOQPG_RS15990 - 3722696..3723064 (+) 369 WP_420615942.1 type IV pilus modification PilV family protein -
  ACOQPG_RS15995 - 3723061..3723909 (+) 849 WP_420615943.1 hypothetical protein -

Sequence

Protein


Download         Length: 362 a.a.        Molecular weight: 39903.59 Da        Isoelectric Point: 8.5453

>NTDB_id=1171154 ACOQPG_RS15965 WP_420615937.1 3718775..3719863(+) (pilT) [MAG: Candidatus Palauibacter sp. isolate c5de0401-b7dc-4d82-98b5-efbda7d568cc]
MDARLVELLREMVVRGASDLHLTAGERPKLRIDGDLTDSRADGVLEPEDTAFLGRSMLSSDQRARFAGEPDFDFGFEIPG
LSRFRANLFRQRGTVACAIRRVPVEIPSLRELGVPSVVGRLADKPRGLVLVTGPTGSGKSTTLAAMVDRINTARAGHIVT
IEDPIEFVHPHKRCIVNQREIGQDTPDFANALRYALRQDPDVILIGELRDPETIQAALTVAETGHLALGTLHTRSAAESI
HRIIDAFPAHRQEQVRAQFAHVFEGVITQTLLRRAQGGGRVVACEILVATPAVRALIREAKVHQIHSAMQAARRFGMRTL
NDALHRLYVRRQVDFDECLRITSDAAEFRRMASTSAPDGSAR

Nucleotide


Download         Length: 1089 bp        

>NTDB_id=1171154 ACOQPG_RS15965 WP_420615937.1 3718775..3719863(+) (pilT) [MAG: Candidatus Palauibacter sp. isolate c5de0401-b7dc-4d82-98b5-efbda7d568cc]
ATGGACGCACGTCTGGTTGAACTGCTGAGGGAGATGGTCGTACGGGGTGCGTCCGACCTGCACCTTACGGCGGGCGAACG
CCCGAAGCTCCGGATCGACGGTGACCTCACCGACAGTCGCGCGGACGGCGTGCTGGAACCGGAGGATACCGCGTTTCTCG
GCCGTTCGATGCTCTCCAGCGACCAGCGCGCGCGTTTCGCGGGTGAGCCCGACTTCGATTTCGGCTTCGAGATTCCCGGC
CTCTCCCGCTTTCGCGCAAACCTGTTCCGGCAGCGAGGGACCGTGGCGTGCGCCATCCGCCGGGTGCCGGTGGAGATCCC
GAGCCTGCGCGAACTCGGCGTTCCATCGGTCGTCGGCCGGCTGGCGGACAAACCGCGCGGACTCGTGCTGGTCACGGGCC
CCACCGGCTCGGGCAAATCGACGACTCTGGCGGCGATGGTGGACCGCATCAACACGGCCCGCGCGGGCCACATCGTGACG
ATCGAGGATCCGATCGAATTCGTGCATCCGCACAAACGCTGCATCGTGAACCAGCGCGAGATCGGGCAGGACACGCCCGA
CTTCGCCAACGCCCTCCGTTACGCGCTGCGGCAGGACCCCGACGTCATCCTCATCGGCGAATTGCGGGATCCCGAGACGA
TCCAGGCCGCGCTCACGGTCGCGGAGACCGGGCACCTTGCGCTCGGCACGCTGCATACGCGTTCGGCGGCAGAGTCCATC
CACCGGATCATCGATGCTTTCCCGGCCCACCGGCAGGAGCAGGTGCGCGCGCAGTTCGCCCACGTCTTTGAGGGGGTCAT
CACCCAGACGCTGCTTCGTCGGGCGCAGGGCGGCGGCCGCGTCGTGGCCTGCGAGATTCTCGTCGCGACGCCCGCGGTTC
GGGCTTTGATTCGGGAGGCGAAGGTCCATCAGATCCATTCCGCGATGCAGGCCGCACGCCGGTTCGGCATGCGGACCCTC
AACGATGCGCTCCACCGGCTCTATGTCCGGCGGCAGGTGGACTTCGACGAATGTCTCCGGATCACCTCCGATGCCGCCGA
ATTCCGTCGCATGGCGAGCACGTCCGCGCCGGACGGATCCGCACGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-245)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.42

97.238

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.702

92.818

0.461
  pilT Vibrio cholerae strain A1552

49.702

92.818

0.461
  pilT Pseudomonas aeruginosa PAK

50.613

90.055

0.456
  pilT Acinetobacter baumannii D1279779

50

90.055

0.45
  pilT Acinetobacter nosocomialis M2

50

90.055

0.45
  pilT Acinetobacter baumannii strain A118

50

90.055

0.45
  pilT Acinetobacter baylyi ADP1

48.64

91.436

0.445
  pilT Legionella pneumophila strain Lp02

49.536

89.227

0.442
  pilT Legionella pneumophila strain ERS1305867

49.536

89.227

0.442
  pilT Pseudomonas stutzeri DSM 10701

49.08

90.055

0.442
  pilT Neisseria meningitidis 8013

50.161

85.912

0.431
  pilT Neisseria gonorrhoeae MS11

49.839

85.912

0.428
  pilU Vibrio cholerae strain A1552

41.408

98.066

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.58

95.304

0.387
  pilU Acinetobacter baylyi ADP1

39.053

93.37

0.365

Multiple sequence alignment