Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACOOHF_RS09060 Genome accession   NZ_OZ244149
Coordinates   2069990..2071111 (+) Length   373 a.a.
NCBI ID   WP_420464269.1    Uniprot ID   -
Organism   MAG: Candidatus Palauibacter sp. isolate 0d4ab03e-7888-48a4-aad6-e62ffb5e22cc     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2064990..2076111
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACOOHF_RS09040 tdh 2065652..2066683 (-) 1032 WP_419934681.1 L-threonine 3-dehydrogenase -
  ACOOHF_RS09045 - 2066867..2067550 (+) 684 WP_419934682.1 energy transducer TonB family protein -
  ACOOHF_RS09050 - 2067691..2068416 (+) 726 WP_419934683.1 ComEA family DNA-binding protein -
  ACOOHF_RS09055 - 2068413..2069993 (+) 1581 WP_419934684.1 GspE/PulE family protein -
  ACOOHF_RS09060 pilT 2069990..2071111 (+) 1122 WP_420464269.1 type IV pilus twitching motility protein PilT Machinery gene
  ACOOHF_RS09065 - 2071108..2072226 (+) 1119 WP_420464270.1 type II secretion system F family protein -
  ACOOHF_RS09070 - 2072286..2072726 (+) 441 WP_420464271.1 hypothetical protein -
  ACOOHF_RS09075 - 2072844..2073254 (+) 411 WP_419934688.1 type IV pilin protein -
  ACOOHF_RS09080 - 2073482..2073919 (+) 438 WP_419934689.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACOOHF_RS09085 - 2073916..2074308 (+) 393 WP_420464272.1 hypothetical protein -
  ACOOHF_RS09090 - 2074305..2075156 (+) 852 WP_420464273.1 PilW family protein -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 40837.98 Da        Isoelectric Point: 10.4207

>NTDB_id=1171006 ACOOHF_RS09060 WP_420464269.1 2069990..2071111(+) (pilT) [MAG: Candidatus Palauibacter sp. isolate 0d4ab03e-7888-48a4-aad6-e62ffb5e22cc]
MMAGTPVTDARLRALLDEMVERDASDLHLTVGGTPKLRIDGRMTDCRTEGVPGSVLRSEDTAALGRSLLTEPQRARFARD
HDIDFSFSVGKLSRFRVNLFRQRGNVACAIRRVPVEIRSLRELGVPSVVARLTEKPRGLVLVTGPTGSGKSTTLAAMVDR
INTRRAGHIITIEDPIEFVHPHKGCIVNQREVGHDTRSFDTALKYALRQDPDVILIGEMRDAETIQAALTIAETGHLALA
TLHTNSAPETINRIIDAFPAHRQEQVRAQFALVFEGVVTQALVPRAGGRGRVAACEILIATPATRALIREGKAHQLCSAM
QAARKFGMQTLNDALFRLYVRRQVAFEDCIRVTSDPAGFRRMTGTSLPAGAAR

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=1171006 ACOOHF_RS09060 WP_420464269.1 2069990..2071111(+) (pilT) [MAG: Candidatus Palauibacter sp. isolate 0d4ab03e-7888-48a4-aad6-e62ffb5e22cc]
ATGATGGCCGGGACTCCCGTCACGGATGCGCGCCTGCGGGCGCTCCTCGACGAGATGGTCGAGCGTGACGCGTCCGACCT
GCACCTCACGGTCGGCGGTACGCCGAAGCTCCGCATCGACGGACGGATGACGGACTGTCGAACCGAGGGTGTGCCGGGGT
CCGTGCTGAGGTCGGAGGACACCGCCGCGCTTGGCCGTTCCCTGCTCACGGAGCCGCAGAGGGCGCGCTTCGCACGGGAC
CACGACATCGACTTCAGCTTCTCGGTGGGGAAGCTGTCCCGCTTCCGCGTGAATCTGTTCCGGCAGCGGGGCAACGTGGC
CTGCGCGATTCGCCGCGTCCCGGTCGAGATCCGGAGCCTGCGGGAACTCGGCGTCCCGTCCGTCGTCGCGCGCCTCACCG
AGAAGCCGCGCGGGCTCGTGCTCGTGACGGGGCCCACCGGCTCCGGCAAGTCGACGACCCTGGCGGCGATGGTCGACCGC
ATCAATACGCGGCGGGCGGGCCACATCATCACCATCGAAGACCCGATCGAGTTCGTGCACCCCCACAAGGGCTGCATCGT
GAACCAGCGCGAGGTCGGACACGACACGCGCAGCTTCGACACGGCGCTCAAGTACGCGCTCCGACAGGATCCCGACGTCA
TCCTGATCGGCGAAATGCGGGACGCGGAAACGATCCAGGCGGCGCTCACGATCGCGGAGACCGGCCACCTCGCGCTCGCC
ACGCTCCACACCAACTCGGCGCCCGAGACGATCAACCGCATCATCGACGCCTTCCCCGCCCACCGGCAGGAGCAGGTCCG
GGCTCAGTTCGCCCTCGTGTTCGAGGGGGTGGTAACGCAGGCGCTGGTCCCGCGGGCGGGCGGCAGGGGGCGCGTCGCCG
CCTGCGAGATCCTCATCGCGACCCCTGCGACCCGGGCTCTGATCCGGGAGGGGAAGGCTCACCAGCTCTGCTCGGCGATG
CAGGCGGCCCGGAAGTTCGGGATGCAGACGCTCAACGACGCCCTCTTCCGGCTCTACGTCCGGCGCCAGGTGGCGTTCGA
GGACTGCATCCGCGTCACGTCGGATCCGGCCGGCTTCCGGCGCATGACCGGGACCTCGCTGCCCGCGGGGGCCGCCCGGT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(58-256)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.829

97.051

0.493
  pilT Pseudomonas aeruginosa PAK

48.116

92.493

0.445
  pilT Acinetobacter baylyi ADP1

47.246

92.493

0.437
  pilT Acinetobacter nosocomialis M2

46.667

92.493

0.432
  pilT Acinetobacter baumannii D1279779

46.667

92.493

0.432
  pilT Acinetobacter baumannii strain A118

46.667

92.493

0.432
  pilT Legionella pneumophila strain ERS1305867

49.691

86.863

0.432
  pilT Legionella pneumophila strain Lp02

49.691

86.863

0.432
  pilT Pseudomonas stutzeri DSM 10701

47.619

90.08

0.429
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.318

87.668

0.424
  pilT Vibrio cholerae strain A1552

48.318

87.668

0.424
  pilT Neisseria gonorrhoeae MS11

48.113

85.255

0.41
  pilT Neisseria meningitidis 8013

48.113

85.255

0.41
  pilU Pseudomonas stutzeri DSM 10701

44.838

90.885

0.408
  pilU Vibrio cholerae strain A1552

41.972

95.174

0.399
  pilU Acinetobacter baylyi ADP1

42.363

93.029

0.394

Multiple sequence alignment