Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACNA18_RS05300 Genome accession   NZ_OZ223661
Coordinates   1009974..1011023 (+) Length   349 a.a.
NCBI ID   WP_049686223.1    Uniprot ID   A0A097ARB7
Organism   Thermoanaerobacter kivui strain Ech2KI isolate Ech2KI     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1004974..1016023
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACNA18_RS05280 sigK 1006085..1006780 (+) 696 WP_049685116.1 RNA polymerase sporulation sigma factor SigK -
  ACNA18_RS05285 - 1006844..1007350 (+) 507 WP_049685117.1 YqeG family HAD IIIA-type phosphatase -
  ACNA18_RS05290 aroE 1007334..1008188 (+) 855 WP_049685118.1 shikimate dehydrogenase -
  ACNA18_RS05295 gspE 1008292..1009968 (+) 1677 WP_049686222.1 type II secretion system ATPase GspE -
  ACNA18_RS05300 pilT 1009974..1011023 (+) 1050 WP_049686223.1 type IV pilus twitching motility protein PilT Machinery gene
  ACNA18_RS05305 - 1011034..1012245 (+) 1212 WP_049685119.1 type II secretion system F family protein -
  ACNA18_RS05310 - 1012269..1012646 (+) 378 WP_049685120.1 competence type IV pilus major pilin ComGC -
  ACNA18_RS05315 - 1012725..1013483 (+) 759 WP_049685121.1 prepilin peptidase -
  ACNA18_RS05320 - 1013488..1013946 (+) 459 WP_049685122.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACNA18_RS05325 - 1013957..1014628 (+) 672 WP_049685123.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ACNA18_RS05330 - 1014619..1015131 (+) 513 WP_049685124.1 PilW family protein -
  ACNA18_RS05335 - 1015121..1015780 (+) 660 WP_049685125.1 pilus assembly PilX N-terminal domain-containing protein -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38685.88 Da        Isoelectric Point: 7.2637

>NTDB_id=1170687 ACNA18_RS05300 WP_049686223.1 1009974..1011023(+) (pilT) [Thermoanaerobacter kivui strain Ech2KI isolate Ech2KI]
MNTKELLKLVVERKASDLHITVGVPPVLRINGYLEKLEGEPFTSQQTEEIVKDLLTSEQLHRLEQNGDIDLSYSVTGLGR
FRINVYKQRGSYSLAIRSVALRIPSIEELGLPPIVKDLAMKARGLILVTGPTGSGKSTTLASMVDLINSKRTCHILTLED
PIEYLHKHNKSIVNQREIGHDAASYASALRAALREDPDVILVGEMRDLETIQIAITAAETGHLVLSTLHTIGAVKTIDRI
IDVFPPHQQQQIKIQLSNVLEGIISQQLIPKKDNSGRVVATEIMIATPAIRNLIREGKTFQIQSAVQTGSKFGMMTMDMS
ILHLLKRGVISLEDALTYCVDQENFSRMI

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=1170687 ACNA18_RS05300 WP_049686223.1 1009974..1011023(+) (pilT) [Thermoanaerobacter kivui strain Ech2KI isolate Ech2KI]
ATGAACACAAAAGAACTTTTAAAGCTTGTAGTAGAGAGAAAAGCTTCAGATTTGCACATAACTGTTGGAGTACCTCCAGT
GCTTAGAATTAATGGTTATTTAGAAAAATTAGAGGGAGAACCTTTTACTTCTCAACAGACAGAAGAGATAGTAAAAGACT
TGCTTACAAGTGAACAATTACATAGGTTAGAGCAAAATGGCGATATAGACTTATCCTACTCTGTGACAGGGCTTGGAAGA
TTTAGAATAAATGTTTACAAGCAAAGAGGAAGTTATAGCCTTGCGATAAGGTCGGTAGCGTTGAGAATTCCTTCTATAGA
AGAGCTGGGACTTCCTCCAATAGTTAAAGACCTAGCCATGAAGGCGAGAGGGCTTATATTGGTAACAGGACCTACTGGCA
GTGGTAAATCTACTACATTGGCTTCTATGGTAGACCTTATAAATTCTAAAAGGACTTGTCACATTTTGACTCTTGAAGAC
CCTATAGAATATTTACATAAACACAACAAAAGCATAGTAAATCAAAGAGAGATTGGGCATGATGCGGCATCTTACGCTTC
AGCTTTGAGGGCTGCTTTGAGGGAAGACCCCGATGTGATATTAGTTGGAGAGATGAGAGACTTAGAGACGATTCAGATTG
CTATAACTGCTGCTGAGACGGGTCATCTTGTTTTGTCGACTCTTCACACAATAGGAGCTGTAAAGACGATCGATAGGATT
ATTGATGTTTTTCCTCCCCATCAACAGCAGCAGATAAAAATACAACTTTCCAACGTGCTTGAGGGGATTATTTCTCAACA
GCTCATACCAAAAAAAGACAATAGCGGTAGAGTAGTTGCAACAGAAATTATGATTGCCACTCCTGCGATAAGAAATCTCA
TAAGAGAAGGAAAAACTTTCCAGATACAATCTGCTGTACAAACAGGTAGCAAGTTTGGAATGATGACAATGGATATGTCT
ATTTTACATCTGTTAAAAAGAGGAGTTATTTCTTTGGAGGATGCATTGACCTATTGTGTGGACCAAGAGAATTTTTCTCG
CATGATATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A097ARB7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

48.986

98.854

0.484
  pilT Acinetobacter baumannii D1279779

48.696

98.854

0.481
  pilT Legionella pneumophila strain Lp02

48.696

98.854

0.481
  pilT Legionella pneumophila strain ERS1305867

48.696

98.854

0.481
  pilT Acinetobacter baumannii strain A118

48.696

98.854

0.481
  pilT Acinetobacter nosocomialis M2

48.696

98.854

0.481
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.547

98.567

0.479
  pilT Pseudomonas aeruginosa PAK

48.406

98.854

0.479
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.802

95.702

0.467
  pilT Vibrio cholerae strain A1552

48.802

95.702

0.467
  pilT Pseudomonas stutzeri DSM 10701

46.667

98.854

0.461
  pilT Neisseria meningitidis 8013

45.954

99.14

0.456
  pilT Neisseria gonorrhoeae MS11

45.665

99.14

0.453
  pilU Pseudomonas stutzeri DSM 10701

40.115

100

0.401
  pilU Vibrio cholerae strain A1552

39.255

100

0.393
  pilU Acinetobacter baylyi ADP1

39.306

99.14

0.39

Multiple sequence alignment