Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ACMTA2_RS30745 Genome accession   NZ_OZ221631
Coordinates   6959344..6960378 (-) Length   344 a.a.
NCBI ID   WP_413793075.1    Uniprot ID   -
Organism   Pseudomonas sp. N4 isolate ena-SAMPLE-TAB-27-01-2025-11:39:45:924-2052     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6954344..6965378
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMTA2_RS30715 ruvX 6954456..6954893 (+) 438 WP_039590497.1 Holliday junction resolvase RuvX -
  ACMTA2_RS30720 pyrR 6954978..6955484 (+) 507 WP_413793072.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  ACMTA2_RS30725 - 6955509..6956513 (+) 1005 WP_003177495.1 aspartate carbamoyltransferase catalytic subunit -
  ACMTA2_RS30730 - 6956510..6957781 (+) 1272 WP_413793073.1 dihydroorotase -
  ACMTA2_RS30735 - 6957905..6958333 (-) 429 WP_053188929.1 NINE protein -
  ACMTA2_RS30740 - 6958583..6959194 (+) 612 WP_413793074.1 C40 family peptidase -
  ACMTA2_RS30745 pilT 6959344..6960378 (-) 1035 WP_413793075.1 type IV pilus twitching motility protein PilT Machinery gene
  ACMTA2_RS30750 - 6960435..6961121 (+) 687 WP_413793076.1 YggS family pyridoxal phosphate-dependent enzyme -
  ACMTA2_RS30755 proC 6961157..6961975 (+) 819 WP_413793077.1 pyrroline-5-carboxylate reductase -
  ACMTA2_RS30760 - 6961986..6962576 (+) 591 WP_413793078.1 YggT family protein -
  ACMTA2_RS30765 metX 6962849..6963988 (+) 1140 WP_003206490.1 homoserine O-succinyltransferase MetX -
  ACMTA2_RS30770 metW 6963996..6964616 (+) 621 WP_413793079.1 methionine biosynthesis protein MetW -
  ACMTA2_RS30775 - 6964642..6965076 (+) 435 WP_413793080.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38095.72 Da        Isoelectric Point: 7.0158

>NTDB_id=1170564 ACMTA2_RS30745 WP_413793075.1 6959344..6960378(-) (pilT) [Pseudomonas sp. N4 isolate ena-SAMPLE-TAB-27-01-2025-11:39:45:924-2052]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDHKQVHELIYDIMNDRQRVDYEKFLETDFSFDVPGIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGMGEVFRKVTEAPRGLVLVTGPTGSGKSTTLAAMIDHLNSHKHHHILTIED
PIEFVHEPRKCLINQREVHRDTQGFSTALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPGDEKSMVRSMLSESLQAVISQTLVKKIGGGRIAAHEIMLGTSAIRNLIREDKVAQMYSSIQTGGSLGMQTLDMCL
KDLVGKGLISREHARERARTPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1170564 ACMTA2_RS30745 WP_413793075.1 6959344..6960378(-) (pilT) [Pseudomonas sp. N4 isolate ena-SAMPLE-TAB-27-01-2025-11:39:45:924-2052]
ATGGATATCACTGAACTGCTGGCGTTCAGCGCCAAACAGGGGGCGTCGGACCTGCACCTGTCCGCCGGGCTGCCGCCGAT
GATCCGCGTGGATGGCGATGTACGGCGGATCAACCTGCCGGCGCTGGACCACAAGCAGGTCCACGAGTTGATCTACGACA
TCATGAACGATCGGCAACGAGTGGATTACGAAAAGTTTCTGGAGACCGACTTTTCCTTCGACGTGCCGGGCATCGCGCGG
TTTCGGGTCAACGCCTTCAACCAGAATCGTGGCGCGGGGGCGGTGTTCCGGACCATTCCGTCGAAAGTCCTGACCATGGA
AGACCTGGGAATGGGCGAGGTGTTTCGCAAGGTGACCGAGGCGCCTCGCGGGCTGGTGCTGGTGACCGGGCCGACCGGCT
CAGGCAAGTCCACCACTCTGGCGGCCATGATCGACCACCTCAACAGCCACAAGCACCACCATATCCTCACCATCGAAGAC
CCTATCGAATTCGTTCACGAACCGCGCAAATGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCCAGGGTTTCTCCAC
AGCCCTGCGCTCGGCCCTGCGGGAGGACCCGGACGTGATCCTGGTGGGTGAGATGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCCGAAACCGGGCACCTGGTGTTCGGCACCCTGCACACCACGTCAGCGGCCAAGACCATCGACCGGGTG
GTGGACGTGTTCCCCGGTGACGAGAAGTCCATGGTTCGCTCGATGTTGTCGGAATCGTTGCAGGCGGTGATTTCCCAGAC
GCTGGTCAAGAAAATCGGCGGGGGTCGGATCGCGGCGCATGAAATCATGCTGGGGACCTCGGCCATCCGGAACCTGATCC
GTGAAGACAAGGTGGCGCAAATGTACTCGTCCATCCAGACCGGCGGCTCGCTGGGGATGCAGACACTGGACATGTGCCTG
AAGGACCTGGTGGGCAAGGGCTTGATCAGCCGTGAACATGCCCGGGAGCGGGCGCGTACGCCGGATAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

88.081

100

0.881
  pilT Pseudomonas aeruginosa PAK

87.791

100

0.878
  pilT Acinetobacter baumannii D1279779

78.779

100

0.788
  pilT Acinetobacter baumannii strain A118

78.779

100

0.788
  pilT Acinetobacter nosocomialis M2

78.779

100

0.788
  pilT Acinetobacter baylyi ADP1

78.198

100

0.782
  pilT Legionella pneumophila strain Lp02

72.189

98.256

0.709
  pilT Legionella pneumophila strain ERS1305867

72.189

98.256

0.709
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.656

97.965

0.663
  pilT Vibrio cholerae strain A1552

67.656

97.965

0.663
  pilT Neisseria meningitidis 8013

64.928

100

0.651
  pilT Neisseria gonorrhoeae MS11

64.638

100

0.648
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.884

100

0.52
  pilU Acinetobacter baylyi ADP1

39.884

100

0.401
  pilU Pseudomonas stutzeri DSM 10701

40.896

97.384

0.398
  pilU Vibrio cholerae strain A1552

39.42

100

0.395

Multiple sequence alignment