Detailed information NTG1170497

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: ACH3IY_RS02215
• Genome accession: NZ_OZ220194
• Coordinates: 149532..150560 (+)
• Length: 342 a.a.
• NCBI ID: WP_146420883.1
• Uniprot ID: -
• Organism: MAG: Francisella salimarina isolate cc853e76-0fc4-44c8-9d05-1aac9db74c27
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 144532..155560

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACH3IY_RS02200	-	145206..145583 (-)	378	WP_395166814.1	GtrA family protein	-
ACH3IY_RS02205	-	145654..146532 (+)	879	WP_395166815.1	Dyp-type peroxidase	-
ACH3IY_RS02210	acnA	146637..149441 (+)	2805	WP_395166816.1	aconitate hydratase AcnA	-
ACH3IY_RS02215	pilT	149532..150560 (+)	1029	WP_146420883.1	type IV pilus twitching motility protein PilT	Machinery gene
ACH3IY_RS02220	-	150597..151577 (-)	981	WP_395166817.1	NAD(P)/FAD-dependent oxidoreductase	-
ACH3IY_RS02225	-	151574..152020 (-)	447	WP_395166818.1	DUF3429 domain-containing protein	-
ACH3IY_RS02230	-	152031..152990 (-)	960	WP_395166819.1	cytochrome d ubiquinol oxidase subunit II	-
ACH3IY_RS02235	-	152993..154369 (-)	1377	WP_146420879.1	cytochrome ubiquinol oxidase subunit I	-
ACH3IY_RS02240	-	154590..155471 (+)	882	WP_395166820.1	TIGR01777 family oxidoreductase	-

Sequence

Protein

• Length: 342 a.a.
• Molecular weight: 38000.82 Da
• Isoelectric Point: 7.0357

>NTDB_id=1170497 ACH3IY_RS02215 WP_146420883.1 149532..150560(+) (pilT) [MAG: Francisella salimarina isolate cc853e76-0fc4-44c8-9d05-1aac9db74c27]
MIRKLLTLCVQKKASDLHLSSGCKAKYRIDGDLIDIETSPVLNDKMISQMLLEIMTDDQKDELVETYECDFSIDDKDNDA
RFRVNAFFHNRGYGAVFRRLENTIPTLDQFGAPKILKTVQAKKGGLILVTGPTGSGKSSTLAALVNEINQKEDSHILTIE
DPVEFVHTSQRALVNQREVKRDTRSFNAALKSALREDPDCILVGEMRDLETIRLALEAAETGHLVLGTLHTMSAIKTVDR
VISVFPPAEQELVRNMLAESLQIVISQRLLKRKGGGRVAGYEVLISNTGIRNMIKENKLSQIYTALQTGTAKGMATMEQS
IENLLKAGTITPEEAAKYIIIR

Nucleotide

• Length: 1029 bp

>NTDB_id=1170497 ACH3IY_RS02215 WP_146420883.1 149532..150560(+) (pilT) [MAG: Francisella salimarina isolate cc853e76-0fc4-44c8-9d05-1aac9db74c27]
ATGATAAGAAAATTATTAACTCTGTGTGTGCAAAAAAAAGCGTCAGACTTACATTTATCATCTGGTTGTAAAGCTAAGTA
TAGAATTGATGGGGACTTGATTGATATCGAAACATCTCCTGTATTAAATGATAAGATGATCTCTCAAATGCTCTTGGAGA
TTATGACAGATGATCAGAAGGATGAGCTTGTAGAGACCTATGAATGTGATTTTTCAATTGATGATAAAGATAATGATGCA
AGATTTAGGGTTAATGCATTTTTTCATAATCGTGGGTATGGTGCTGTATTTCGTCGTTTAGAGAATACTATCCCCACTCT
AGATCAGTTCGGGGCTCCTAAGATTCTAAAAACAGTTCAAGCTAAAAAGGGTGGTTTGATACTTGTTACTGGACCTACAG
GTTCTGGTAAAAGTAGTACATTAGCAGCTTTAGTAAATGAGATAAACCAAAAAGAAGATTCTCATATCCTAACGATTGAA
GACCCTGTTGAATTTGTACATACAAGCCAAAGAGCTTTAGTTAACCAACGTGAGGTCAAAAGAGATACAAGAAGCTTTAA
TGCGGCTTTGAAATCAGCACTAAGGGAAGATCCTGATTGTATCCTTGTTGGGGAGATGCGTGATTTAGAAACTATACGTT
TGGCTCTTGAAGCTGCTGAAACAGGCCACTTGGTACTAGGAACATTACATACAATGTCAGCAATCAAAACAGTGGATAGG
GTAATATCGGTATTTCCACCAGCTGAGCAGGAGCTAGTCCGTAATATGTTAGCAGAATCTTTACAGATTGTAATATCTCA
GCGTCTTCTTAAGCGTAAAGGTGGTGGTCGTGTTGCTGGATATGAGGTTCTTATTTCTAATACTGGTATCAGAAATATGA
TAAAAGAGAATAAGCTTTCTCAAATATACACGGCTCTTCAGACTGGTACGGCAAAAGGTATGGCAACTATGGAGCAAAGT
ATAGAAAATCTTCTTAAAGCGGGAACAATAACTCCTGAAGAGGCAGCTAAGTATATAATAATAAGATAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (9-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Acinetobacter nosocomialis M2	55.988	97.661	0.547
pilT	Acinetobacter baumannii strain A118	55.988	97.661	0.547
pilT	Acinetobacter baumannii D1279779	55.988	97.661	0.547
pilT	Legionella pneumophila strain Lp02	54.142	98.83	0.535
pilT	Legionella pneumophila strain ERS1305867	54.142	98.83	0.535
pilT	Neisseria meningitidis 8013	54.006	98.538	0.532
pilT	Neisseria gonorrhoeae MS11	54.006	98.538	0.532
pilT	Pseudomonas aeruginosa PAK	54.491	97.661	0.532
pilT	Acinetobacter baylyi ADP1	55.319	96.199	0.532
pilT	Vibrio cholerae strain A1552	53.846	98.83	0.532
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	53.846	98.83	0.532
pilT	Pseudomonas stutzeri DSM 10701	53.892	97.661	0.526
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.713	96.784	0.433
pilU	Pseudomonas stutzeri DSM 10701	38.739	97.368	0.377
pilU	Acinetobacter baylyi ADP1	38.209	97.953	0.374
pilU	Vibrio cholerae strain A1552	38.889	94.737	0.368