Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   AB8516_RS13585 Genome accession   NZ_OZ156465
Coordinates   3098637..3099746 (-) Length   369 a.a.
NCBI ID   WP_369161418.1    Uniprot ID   -
Organism   Candidatus Thiodiazotropha sp. LNASS1 isolate 6c41e2f9-faec-415b-97c3-6ddcd12de2e9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3093637..3104746
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AB8516_RS13565 - 3095462..3096772 (+) 1311 WP_369161410.1 DUF1329 domain-containing protein -
  AB8516_RS13570 - 3096836..3097432 (+) 597 WP_369161412.1 2-hydroxychromene-2-carboxylate isomerase -
  AB8516_RS13575 - 3097429..3098073 (+) 645 WP_369161414.1 glutathione S-transferase family protein -
  AB8516_RS13580 - 3098086..3098646 (+) 561 WP_369161416.1 antibiotic biosynthesis monooxygenase -
  AB8516_RS13585 pilU 3098637..3099746 (-) 1110 WP_369161418.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  AB8516_RS13590 - 3099974..3101131 (-) 1158 WP_369161420.1 FAD-dependent oxidoreductase -
  AB8516_RS13595 - 3101216..3101386 (-) 171 WP_369161422.1 rubredoxin -
  AB8516_RS13600 - 3101512..3101871 (-) 360 WP_369161424.1 nucleotide pyrophosphohydrolase -
  AB8516_RS13605 - 3101905..3102858 (-) 954 WP_369161426.1 zinc metalloprotease HtpX -
  AB8516_RS13610 - 3103044..3103352 (+) 309 WP_369161428.1 hypothetical protein -
  AB8516_RS13615 - 3103441..3103827 (-) 387 WP_369163289.1 VOC family protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41054.07 Da        Isoelectric Point: 6.3724

>NTDB_id=1167921 AB8516_RS13585 WP_369161418.1 3098637..3099746(-) (pilU) [Candidatus Thiodiazotropha sp. LNASS1 isolate 6c41e2f9-faec-415b-97c3-6ddcd12de2e9]
MDIVPYLKLMVQKNGSDLFFSTGAAPHLKTEGETRPIGSSPMQSGQVRKLAYGIMSDDQIKEYEATYECNLAISVNNLGR
FRVNVYKQRGDSAMVIRYIKGVIPPVEKLNLPIILKDLIMEPRGLILVVGATGSGKSTTLASMIEHRNQSIAGHILTIED
PIEYLYTHKKSVVDQREVGLDTLSYENALKNAMREAPDVIQIGEIREMSTMQHAIAYAETGHLALSTLHANNSNQALDRI
INFFPDSAHHQLYMDLSLNLRAVISQRLVKGINGQRIPAVEIMLLSTYISELIQKGDIHTIKEVMEQGTERGMQTFDQSL
YKLYKEGKISMEEALSHADSRNNLSLKIRLSDTEGVEAPGGLGVAEDHF

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1167921 AB8516_RS13585 WP_369161418.1 3098637..3099746(-) (pilU) [Candidatus Thiodiazotropha sp. LNASS1 isolate 6c41e2f9-faec-415b-97c3-6ddcd12de2e9]
ATGGATATCGTGCCCTATCTCAAGCTGATGGTGCAAAAAAACGGCTCAGATCTCTTTTTCAGCACCGGTGCCGCCCCCCA
CCTGAAAACCGAAGGTGAAACTCGTCCGATAGGGTCTTCCCCAATGCAAAGCGGGCAGGTCAGGAAACTCGCTTACGGCA
TCATGAGCGATGATCAGATCAAGGAGTACGAAGCCACTTACGAGTGCAATCTGGCGATTTCCGTCAACAACCTTGGGCGC
TTCCGCGTCAATGTCTACAAACAACGGGGTGATTCAGCGATGGTCATCCGCTATATCAAGGGCGTGATCCCCCCAGTAGA
GAAGCTCAACCTGCCGATTATTCTGAAGGACCTGATCATGGAACCCCGCGGCCTGATCCTGGTCGTCGGCGCGACAGGCT
CAGGCAAGTCGACAACCCTCGCCTCGATGATCGAACACCGCAATCAAAGCATAGCCGGACATATATTGACCATTGAGGAC
CCTATCGAATATCTCTACACCCACAAAAAGTCGGTGGTCGATCAGCGTGAGGTCGGACTCGACACGCTCTCATACGAAAA
CGCCCTGAAAAATGCCATGCGTGAGGCCCCTGATGTGATTCAGATCGGTGAGATCCGGGAAATGAGCACCATGCAGCACG
CCATCGCCTATGCTGAAACAGGCCACCTTGCGCTCTCGACGCTGCACGCCAACAACTCAAACCAGGCACTCGATCGCATC
ATCAACTTCTTTCCAGACAGCGCCCATCACCAGCTGTATATGGACCTCTCTCTCAATCTTCGGGCAGTCATATCCCAACG
CCTGGTAAAGGGTATCAACGGTCAACGCATCCCCGCGGTCGAGATCATGCTGTTATCGACCTACATCTCCGAACTGATTC
AGAAAGGGGATATTCACACCATCAAAGAGGTCATGGAACAAGGCACCGAACGCGGCATGCAGACCTTCGATCAATCCCTC
TACAAGCTCTACAAAGAGGGCAAGATATCTATGGAAGAGGCGCTCTCCCATGCGGATTCACGCAACAATCTGTCGCTGAA
GATACGCCTCTCCGACACCGAGGGCGTGGAAGCCCCGGGCGGCCTGGGTGTGGCAGAGGATCATTTCTGA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(55-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

51.261

96.748

0.496
  pilU Acinetobacter baylyi ADP1

50.847

95.935

0.488
  pilU Vibrio cholerae strain A1552

48.286

94.851

0.458
  pilT Acinetobacter baylyi ADP1

42.353

92.141

0.39
  pilT Legionella pneumophila strain ERS1305867

42.814

90.515

0.388
  pilT Legionella pneumophila strain Lp02

42.814

90.515

0.388
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.369

91.057

0.377
  pilT Vibrio cholerae strain A1552

41.369

91.057

0.377
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.708

91.87

0.374
  pilT Acinetobacter baumannii D1279779

40.533

91.599

0.371
  pilT Acinetobacter nosocomialis M2

40.533

91.599

0.371
  pilT Acinetobacter baumannii strain A118

40.533

91.599

0.371
  pilT Pseudomonas aeruginosa PAK

39.826

93.225

0.371
  pilT Neisseria gonorrhoeae MS11

39.193

94.038

0.369
  pilT Neisseria meningitidis 8013

39.193

94.038

0.369
  pilT Pseudomonas stutzeri DSM 10701

39.244

93.225

0.366

Multiple sequence alignment