Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   Z042_RS08585 Genome accession   NZ_CP007044
Coordinates   1936324..1937328 (+) Length   334 a.a.
NCBI ID   WP_024914281.1    Uniprot ID   W0L6V9
Organism   Chania multitudinisentens RB-25     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1931324..1942328
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Z042_RS08555 (Z042_07340) hemW 1932070..1933221 (-) 1152 WP_037407530.1 radical SAM family heme chaperone HemW -
  Z042_RS08560 (Z042_07335) - 1933211..1933807 (-) 597 WP_024914276.1 XTP/dITP diphosphatase -
  Z042_RS08565 (Z042_07330) yggU 1933825..1934115 (-) 291 WP_024914277.1 DUF167 family protein YggU -
  Z042_RS08570 (Z042_07325) - 1934112..1934666 (-) 555 WP_024914278.1 YggT family protein -
  Z042_RS08575 (Z042_07320) proC 1934746..1935567 (-) 822 WP_024914279.1 pyrroline-5-carboxylate reductase -
  Z042_RS08580 (Z042_07315) - 1935602..1936303 (-) 702 WP_024914280.1 YggS family pyridoxal phosphate-dependent enzyme -
  Z042_RS08585 (Z042_07310) pilT 1936324..1937328 (+) 1005 WP_024914281.1 type IV pilus twitching motility protein PilT Machinery gene
  Z042_RS08590 (Z042_07305) ruvX 1937338..1937760 (-) 423 WP_024914282.1 Holliday junction resolvase RuvX -
  Z042_RS08595 (Z042_07300) - 1937760..1938323 (-) 564 WP_024914283.1 YqgE/AlgH family protein -
  Z042_RS08600 (Z042_07295) gshB 1938481..1939431 (-) 951 WP_024914284.1 glutathione synthase -
  Z042_RS08605 (Z042_07290) rsmE 1939465..1940196 (-) 732 WP_024914285.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  Z042_RS08610 (Z042_07285) endA 1940390..1941079 (-) 690 WP_024909908.1 deoxyribonuclease I -
  Z042_RS08615 (Z042_07280) - 1941184..1941696 (-) 513 WP_024909909.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 36293.55 Da        Isoelectric Point: 6.4281

>NTDB_id=116301 Z042_RS08585 WP_024914281.1 1936324..1937328(+) (pilT) [Chania multitudinisentens RB-25]
MDIDKLVVLSVKHNASDLHLCTGHQPMLRIDGELRPVEEIEAVSHSQMAGWCETLLTQEQRQQLQGSGQLDLALTLAGGS
RLRANFFLQNGGVSIALRRIASACPTLAELETPEIIPALIQREDGLILVTGATGSGKSTTLAAMIDFINQHQRRHILTLE
DPIEFIHHSQRSLIQQRELGRDTRSFDEALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPVEEKPYVRAQLANSLQAVIAQKLLSKPGGGRVAIYEILTATAAVSSMIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIQAGLLSGAA

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=116301 Z042_RS08585 WP_024914281.1 1936324..1937328(+) (pilT) [Chania multitudinisentens RB-25]
ATGGATATCGATAAGTTGGTGGTTCTTAGTGTAAAACATAATGCCTCCGATCTGCACCTTTGTACCGGTCATCAGCCGAT
GCTGCGTATTGATGGTGAATTGCGGCCAGTAGAAGAAATAGAAGCGGTTTCGCACTCACAGATGGCTGGCTGGTGCGAAA
CATTGTTGACGCAGGAGCAACGGCAGCAGCTACAGGGGAGTGGCCAGCTCGATCTGGCTTTGACATTGGCGGGTGGCAGC
CGCTTGCGCGCCAATTTCTTTTTACAGAATGGCGGGGTGTCCATCGCCTTGCGCCGGATTGCCAGTGCATGCCCGACCTT
GGCCGAACTGGAAACCCCCGAGATCATTCCGGCGCTGATCCAGCGGGAAGATGGCCTGATTTTGGTTACTGGAGCGACTG
GAAGCGGTAAATCCACCACCCTGGCAGCGATGATTGATTTTATCAACCAGCATCAGCGCCGCCATATTCTAACGCTGGAA
GATCCGATTGAGTTTATCCACCACAGCCAGCGTTCCCTGATCCAGCAGCGTGAACTGGGGCGCGATACCCGCAGCTTTGA
TGAGGCACTGCGGGCAGCGCTGCGTGAAGATCCCGATGTGATCCTGCTGGGGGAACTGCGCGATACCGCCACGATCCGGC
TAGCGTTGACTGCGGCAGAAACCGGCCACCTGGTATTGGCGACGTTGCATACCCGCAGTGCGCCGCAGGCGGTAGAACGG
TTGGTGGATGTATTCCCGGTAGAGGAAAAGCCTTACGTTCGGGCGCAACTGGCAAACAGCTTGCAGGCGGTGATTGCGCA
AAAGCTGTTGAGTAAACCTGGCGGTGGGCGGGTGGCAATTTATGAGATCCTCACTGCGACTGCCGCAGTGAGTAGCATGA
TCCGCGAAGGTAAAACGCACCAACTTGCCAGCGTATTGCAAACCGGTGCACAGTCAGGCATGCAGACCTTTGAGCAGGGT
TTACAGCAGCGTATTCAGGCAGGTTTGCTCAGCGGAGCGGCTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB W0L6V9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

53.012

99.401

0.527
  pilT Vibrio cholerae strain A1552

53.012

99.401

0.527
  pilT Acinetobacter baylyi ADP1

51.057

99.102

0.506
  pilT Neisseria meningitidis 8013

50

99.401

0.497
  pilT Acinetobacter baumannii D1279779

50.151

99.102

0.497
  pilT Acinetobacter baumannii strain A118

50.151

99.102

0.497
  pilT Pseudomonas stutzeri DSM 10701

50.151

99.102

0.497
  pilT Acinetobacter nosocomialis M2

50.151

99.102

0.497
  pilT Neisseria gonorrhoeae MS11

49.552

100

0.497
  pilT Pseudomonas aeruginosa PAK

49.547

99.102

0.491
  pilT Legionella pneumophila strain Lp02

48.338

99.102

0.479
  pilT Legionella pneumophila strain ERS1305867

48.338

99.102

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.962

95.509

0.458
  pilU Vibrio cholerae strain A1552

39.815

97.006

0.386
  pilU Acinetobacter baylyi ADP1

39.938

96.707

0.386
  pilU Pseudomonas stutzeri DSM 10701

38.82

96.407

0.374

Multiple sequence alignment