Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   U9974_RS10260 Genome accession   NZ_OY986433
Coordinates   1932875..1933954 (+) Length   359 a.a.
NCBI ID   WP_322774008.1    Uniprot ID   -
Organism   Synechococcus sp. CBW1107 isolate KAC_106     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1927875..1938954
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U9974_RS10240 (ICNINCKA_02033) - 1928761..1929054 (-) 294 WP_322774004.1 sulfurtransferase TusA family protein -
  U9974_RS10245 (ICNINCKA_02034) dnaJ 1929070..1930191 (-) 1122 WP_322774005.1 molecular chaperone DnaJ -
  U9974_RS10250 (ICNINCKA_02035) grpE 1930191..1930907 (-) 717 WP_322774006.1 nucleotide exchange factor GrpE -
  U9974_RS10255 (ICNINCKA_02036) pilB/pilB1 1931010..1932818 (+) 1809 WP_322774007.1 GspE/PulE family protein Machinery gene
  U9974_RS10260 (ICNINCKA_02037) pilT 1932875..1933954 (+) 1080 WP_322774008.1 type IV pilus twitching motility protein PilT Machinery gene
  U9974_RS10265 (ICNINCKA_02038) - 1933967..1935367 (+) 1401 WP_322774009.1 type II secretion system F family protein -
  U9974_RS10270 (ICNINCKA_02039) - 1935332..1936657 (-) 1326 WP_322774010.1 NAD(P)/FAD-dependent oxidoreductase -
  U9974_RS10275 (ICNINCKA_02040) dusA 1936664..1937674 (+) 1011 WP_322774011.1 tRNA dihydrouridine(20/20a) synthase DusA -

Sequence

Protein


Download         Length: 359 a.a.        Molecular weight: 39656.05 Da        Isoelectric Point: 6.3445

>NTDB_id=1162508 U9974_RS10260 WP_322774008.1 1932875..1933954(+) (pilT) [Synechococcus sp. CBW1107 isolate KAC_106]
MELQIEELMEELVEQKGSDLHLSAGQQPYGRFNGELRPMREQALSEESCNRMIFSILTNSQRKQLEQTWELDCSYGLRGL
ARFRINVFSQRGTYAASMRALNSKVPTPQELGLPPVVVDITKRPSGLVLVTGPTGSGKSTTLASLLDHINHSRAKHIITI
EDPIEFAYINDKSLIHQRQLNEDTKSFSNALRAALREDPDVILVGEMRDLETIQLAITAAETGHLVFATLHTSSAAQTVD
RMVDVFPAGQQAQIRTQLSSSLAAIFAQTLCRRSSPSSHNYGRVMAQEIIVNTPALANLIREAKTAQIYSQIQTGGQLGM
QTLEKALADMVKQGDIALEEAHTHSTKPDELNRLLGNSL

Nucleotide


Download         Length: 1080 bp        

>NTDB_id=1162508 U9974_RS10260 WP_322774008.1 1932875..1933954(+) (pilT) [Synechococcus sp. CBW1107 isolate KAC_106]
ATGGAGCTCCAGATCGAGGAGTTGATGGAAGAGCTGGTCGAGCAGAAGGGCAGCGACCTTCATCTCTCGGCCGGCCAGCA
ACCCTACGGACGCTTCAACGGGGAACTCAGGCCGATGCGGGAGCAGGCCCTCAGCGAGGAATCCTGCAACCGCATGATCT
TCTCGATCCTCACCAACTCCCAGCGGAAACAACTGGAGCAGACCTGGGAACTCGATTGCTCCTACGGCCTGCGCGGCCTG
GCCCGCTTCCGCATCAATGTCTTCAGCCAGCGCGGCACCTATGCCGCCTCCATGCGGGCCCTGAACAGCAAGGTGCCCAC
GCCCCAGGAGCTGGGCCTGCCTCCGGTGGTGGTGGACATCACCAAGCGGCCTTCAGGCCTGGTGCTGGTGACGGGTCCGA
CCGGATCGGGCAAAAGCACCACCCTGGCCTCACTGCTCGATCACATCAACCACAGCCGGGCCAAGCACATCATCACGATC
GAAGATCCGATCGAGTTTGCTTACATCAATGACAAGAGCCTGATCCATCAGCGTCAGCTCAATGAAGACACCAAAAGCTT
CAGCAATGCCCTGAGAGCTGCCCTGCGGGAGGATCCCGATGTGATTCTGGTGGGGGAAATGCGTGATCTGGAAACGATTC
AGCTGGCCATCACCGCTGCAGAAACCGGCCACCTGGTGTTCGCCACCCTGCACACCAGTTCCGCCGCCCAGACCGTGGAT
CGCATGGTGGATGTGTTCCCGGCGGGCCAGCAGGCCCAGATCCGCACCCAGCTCAGCAGCAGCCTGGCGGCGATCTTCGC
CCAGACGCTCTGCCGCAGAAGTTCACCCTCCAGCCACAACTATGGTCGGGTGATGGCCCAGGAGATCATTGTCAACACGC
CGGCCCTGGCCAACCTGATCCGCGAAGCGAAAACAGCTCAGATCTATTCGCAGATTCAGACCGGCGGTCAGCTGGGCATG
CAGACCCTGGAGAAAGCCCTGGCCGACATGGTCAAGCAGGGGGACATCGCCCTGGAGGAAGCCCACACCCACTCCACCAA
GCCGGATGAGTTGAACCGGCTGCTCGGCAACTCCCTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

51.576

97.214

0.501
  pilT Pseudomonas stutzeri DSM 10701

51.003

97.214

0.496
  pilT Acinetobacter baylyi ADP1

51.003

97.214

0.496
  pilT Acinetobacter nosocomialis M2

50.43

97.214

0.49
  pilT Acinetobacter baumannii D1279779

50.43

97.214

0.49
  pilT Acinetobacter baumannii strain A118

50.43

97.214

0.49
  pilT Neisseria meningitidis 8013

51.032

94.429

0.482
  pilT Neisseria gonorrhoeae MS11

50.737

94.429

0.479
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.147

94.986

0.476
  pilT Vibrio cholerae strain A1552

50.147

94.986

0.476
  pilT Legionella pneumophila strain ERS1305867

50.294

94.708

0.476
  pilT Legionella pneumophila strain Lp02

50.294

94.708

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.443

98.05

0.465
  pilU Acinetobacter baylyi ADP1

37.5

98.05

0.368
  pilU Pseudomonas stutzeri DSM 10701

37.11

98.329

0.365

Multiple sequence alignment