Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   U9974_RS10255 Genome accession   NZ_OY986433
Coordinates   1931010..1932818 (+) Length   602 a.a.
NCBI ID   WP_322774007.1    Uniprot ID   -
Organism   Synechococcus sp. CBW1107 isolate KAC_106     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1926010..1937818
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U9974_RS10225 (ICNINCKA_02030) murB 1926436..1927389 (+) 954 WP_322774002.1 UDP-N-acetylmuramate dehydrogenase -
  U9974_RS10230 (ICNINCKA_02031) - 1927411..1927746 (+) 336 WP_255103313.1 YbaB/EbfC family nucleoid-associated protein -
  U9974_RS10235 (ICNINCKA_02032) rsgA 1927770..1928771 (-) 1002 WP_322774003.1 ribosome small subunit-dependent GTPase A -
  U9974_RS10240 (ICNINCKA_02033) - 1928761..1929054 (-) 294 WP_322774004.1 sulfurtransferase TusA family protein -
  U9974_RS10245 (ICNINCKA_02034) dnaJ 1929070..1930191 (-) 1122 WP_322774005.1 molecular chaperone DnaJ -
  U9974_RS10250 (ICNINCKA_02035) grpE 1930191..1930907 (-) 717 WP_322774006.1 nucleotide exchange factor GrpE -
  U9974_RS10255 (ICNINCKA_02036) pilB/pilB1 1931010..1932818 (+) 1809 WP_322774007.1 GspE/PulE family protein Machinery gene
  U9974_RS10260 (ICNINCKA_02037) pilT 1932875..1933954 (+) 1080 WP_322774008.1 type IV pilus twitching motility protein PilT Machinery gene
  U9974_RS10265 (ICNINCKA_02038) - 1933967..1935367 (+) 1401 WP_322774009.1 type II secretion system F family protein -
  U9974_RS10270 (ICNINCKA_02039) - 1935332..1936657 (-) 1326 WP_322774010.1 NAD(P)/FAD-dependent oxidoreductase -
  U9974_RS10275 (ICNINCKA_02040) dusA 1936664..1937674 (+) 1011 WP_322774011.1 tRNA dihydrouridine(20/20a) synthase DusA -

Sequence

Protein


Download         Length: 602 a.a.        Molecular weight: 66803.17 Da        Isoelectric Point: 5.5843

>NTDB_id=1162507 U9974_RS10255 WP_322774007.1 1931010..1932818(+) (pilB/pilB1) [Synechococcus sp. CBW1107 isolate KAC_106]
MTLSATRPVPAAETLQQRRLEAELLSGEKLLLPVDLQQADPRAAEACPRIDAQHWREWVALPLVRHGEQLLIAIPSHWQE
QQRQQLEEAVLANGITPQLRLGLQDEISEALQQKMADRRHRPSKDTDLVSQPNALSLLEGLSLEDRLEEAPATEEQELDL
EASLKASNASPVVSLVNRILIQAMESESSDIHVEPEEDGLRIRFRQDGVLHPVEHLPRSLAPAVTSRFKIMAELDIAERR
MPQDGRIRRMFRGRTIELRVSTLPGRWGEKVVLRLLDSGSTRLGLDRLITEPTTLATVRSMGSRPFGMVLVTGPTGSGKS
TTLYSLLAERNEPGINISTVEDPIEYMLKGITQTQVNRDKGYDFSTALRAFMRQDPDVLLVGETRDGETAKTAIEAALTG
HLVLTTLHCNDAPSAIARLNEMGVEPFMVSASLIGIVSQRLLRKVCESCRVPYHPGEEELGHFGLFASQEQSVTFYKANR
GPRADGSSCPTCQGRGYKGRIGVYEVLRINDNLASAIAKGASTDVIRNLSIETGMKTLLVYGLELVRHGQTTLEEVERML
LTDTELESQRRAQALTTLTCQGCGAGLRDEWLECPYCLTPRR

Nucleotide


Download         Length: 1809 bp        

>NTDB_id=1162507 U9974_RS10255 WP_322774007.1 1931010..1932818(+) (pilB/pilB1) [Synechococcus sp. CBW1107 isolate KAC_106]
ATGACCCTCTCGGCGACACGCCCGGTCCCTGCGGCCGAAACGCTCCAGCAGCGGCGCCTGGAGGCCGAGCTGCTCAGCGG
TGAGAAGCTGCTGCTCCCCGTGGACCTGCAGCAGGCCGATCCGCGGGCCGCTGAGGCCTGCCCCAGGATCGATGCCCAGC
ACTGGCGGGAGTGGGTGGCCCTGCCCCTGGTGCGCCATGGAGAGCAGCTCTTGATCGCCATCCCCAGCCACTGGCAGGAG
CAGCAGCGCCAGCAACTGGAGGAGGCGGTGCTGGCCAACGGCATCACCCCGCAGTTGCGCCTGGGGCTGCAGGACGAGAT
CAGCGAGGCCCTGCAGCAGAAGATGGCTGACCGACGCCATCGCCCCAGCAAGGACACCGACCTGGTCTCTCAGCCGAACG
CCCTCTCGCTGCTGGAGGGCCTCAGCCTGGAAGACCGCCTGGAGGAGGCGCCCGCTACCGAGGAGCAGGAACTGGACCTG
GAGGCGAGCCTGAAGGCCTCCAACGCCTCACCGGTGGTGAGCCTGGTGAACCGCATCCTGATCCAGGCGATGGAGTCGGA
GTCGAGCGACATCCACGTGGAGCCGGAGGAGGACGGGCTGCGGATCCGCTTTCGCCAGGACGGAGTGCTGCATCCGGTCG
AACACCTGCCCAGGAGCCTGGCACCGGCGGTCACCTCGCGCTTCAAGATCATGGCCGAACTGGACATCGCCGAGCGGCGG
ATGCCCCAGGACGGGCGCATCCGCCGGATGTTCCGCGGCCGCACGATCGAACTTCGGGTGAGTACTCTGCCGGGCCGCTG
GGGGGAGAAGGTCGTGCTGCGTCTGCTCGACAGCGGCAGCACCCGTCTGGGCCTCGACCGACTGATCACCGAGCCAACCA
CCCTGGCCACCGTGCGATCGATGGGCTCGAGGCCCTTCGGGATGGTGCTGGTGACGGGGCCCACCGGGTCAGGCAAGTCG
ACCACGCTCTATTCCCTGCTGGCGGAACGCAACGAGCCCGGCATCAACATCTCCACCGTCGAAGATCCGATCGAGTACAT
GCTCAAGGGCATCACCCAGACCCAGGTGAACCGCGACAAGGGCTACGACTTCAGCACTGCCCTGCGGGCCTTCATGCGCC
AGGACCCGGACGTGCTGCTGGTGGGGGAAACCCGCGATGGCGAGACGGCCAAGACCGCCATCGAGGCGGCCCTGACCGGC
CATCTGGTGCTCACGACCCTGCACTGCAACGACGCCCCCAGTGCCATCGCCCGGCTCAACGAGATGGGCGTGGAGCCCTT
CATGGTGAGTGCCTCCCTGATCGGGATCGTCTCCCAGCGGCTGCTGCGCAAGGTCTGCGAGTCGTGCCGCGTGCCCTACC
ACCCAGGGGAGGAAGAGCTGGGCCATTTCGGCCTCTTCGCCAGCCAGGAGCAGTCGGTCACCTTCTACAAAGCCAATCGG
GGACCCAGGGCCGACGGCAGCAGCTGTCCCACCTGCCAGGGACGGGGCTACAAGGGCCGCATCGGCGTCTACGAAGTGCT
GAGGATCAACGACAACCTCGCCAGTGCCATCGCCAAGGGCGCCAGCACCGACGTGATCCGCAATCTTTCGATCGAGACCG
GCATGAAAACCCTGCTGGTCTATGGCCTGGAACTGGTGCGCCACGGCCAGACCACCCTCGAGGAAGTGGAGCGGATGCTG
CTCACCGACACCGAACTGGAATCCCAGCGGCGCGCCCAGGCCCTCACCACCTTGACCTGCCAGGGCTGCGGGGCGGGCCT
CAGGGATGAATGGCTGGAGTGCCCCTACTGCCTGACCCCGCGCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(178-444)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(32-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

57.988

84.219

0.488
  pilB Vibrio cholerae strain A1552

42.941

84.718

0.364

Multiple sequence alignment