Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   U9970_RS12480 Genome accession   NZ_OY986431
Coordinates   2302637..2303716 (+) Length   359 a.a.
NCBI ID   WP_254941691.1    Uniprot ID   -
Organism   Cyanobium usitatum str. Tous isolate KAC_108     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2297637..2308716
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U9970_RS12460 (OGCDGJMD_02543) - 2298665..2298925 (-) 261 WP_322764449.1 sulfurtransferase TusA family protein -
  U9970_RS12465 (OGCDGJMD_02544) dnaJ 2298922..2300037 (-) 1116 WP_322764450.1 molecular chaperone DnaJ -
  U9970_RS12470 (OGCDGJMD_02545) grpE 2300037..2300711 (-) 675 WP_322764451.1 nucleotide exchange factor GrpE -
  U9970_RS12475 (OGCDGJMD_02546) pilB/pilB1 2300800..2302629 (+) 1830 WP_322764452.1 GspE/PulE family protein Machinery gene
  U9970_RS12480 (OGCDGJMD_02547) pilT 2302637..2303716 (+) 1080 WP_254941691.1 type IV pilus twitching motility protein PilT Machinery gene
  U9970_RS12485 (OGCDGJMD_02548) - 2303741..2305000 (+) 1260 WP_254941692.1 type II secretion system F family protein -
  U9970_RS12490 (OGCDGJMD_02549) - 2304988..2306256 (-) 1269 WP_322764453.1 NAD(P)/FAD-dependent oxidoreductase -
  U9970_RS12495 (OGCDGJMD_02550) dusA 2306242..2307288 (+) 1047 WP_322764454.1 tRNA dihydrouridine(20/20a) synthase DusA -
  U9970_RS12500 (OGCDGJMD_02551) - 2307285..2307863 (-) 579 WP_322764455.1 RNA-binding protein -

Sequence

Protein


Download         Length: 359 a.a.        Molecular weight: 39462.34 Da        Isoelectric Point: 6.4126

>NTDB_id=1162490 U9970_RS12480 WP_254941691.1 2302637..2303716(+) (pilT) [Cyanobium usitatum str. Tous isolate KAC_108]
MAVMIEDLMTQLVKDGGSDLHISAGIPPYGRFSGQLRPIHDDSLDEESCNKLIFSMLNNAQRKQLEQTWELDCAYGLKGI
ARFRVNVYRQRGTYAACLRALSNTVPTLESLGLPPIVEEMSHMPKGLILVTGPTGSGKTTTLAAMIEHINQTRAEHILTI
EDPIEFTYRPVKSVIHQRQLNEDTRSFANALKAALREDPDVILVGELRDLDTIQLAITAAETGHLVLGTLHTSSAAQTVD
RMVDVFPPSQQNQIRVQLSSSLVAVFAQTLCKRHNPSPGQSGRVMAQEIMINTPATANLIREGKTAQLYSAIQTGGQLSM
QTLEKALGDLVLSNQVMMDEALLKTSKPDELIRLVKQLS

Nucleotide


Download         Length: 1080 bp        

>NTDB_id=1162490 U9970_RS12480 WP_254941691.1 2302637..2303716(+) (pilT) [Cyanobium usitatum str. Tous isolate KAC_108]
ATGGCCGTGATGATTGAAGACTTGATGACCCAACTTGTGAAGGATGGCGGCAGTGATCTCCATATCAGTGCAGGCATACC
TCCCTATGGCAGGTTCAGCGGTCAACTAAGGCCCATACACGATGATTCCCTAGACGAAGAAAGCTGCAATAAACTAATCT
TCTCCATGCTAAATAATGCCCAGCGCAAGCAGCTAGAGCAAACCTGGGAATTAGATTGTGCCTATGGCCTTAAAGGGATC
GCCCGCTTCAGGGTCAATGTTTATCGCCAGAGGGGTACCTACGCCGCCTGCCTGCGAGCGCTTTCCAACACCGTGCCCAC
TCTGGAAAGCCTAGGGCTACCGCCGATTGTGGAGGAAATGAGCCACATGCCCAAAGGGCTGATTCTCGTAACAGGCCCCA
CCGGCTCTGGCAAAACCACAACCCTGGCGGCAATGATCGAGCATATAAATCAAACCCGGGCAGAACATATATTGACCATT
GAAGACCCGATTGAATTCACATACAGACCAGTAAAAAGTGTCATCCACCAGCGCCAGCTCAACGAAGACACCCGCAGCTT
TGCCAATGCCCTAAAGGCAGCCCTTCGAGAAGATCCCGACGTAATCCTGGTGGGCGAGCTTCGAGACCTCGACACCATCC
AGTTAGCAATTACGGCAGCAGAAACCGGTCACCTGGTATTGGGCACCCTGCACACGAGTTCGGCCGCCCAAACCGTGGAC
CGCATGGTGGATGTTTTCCCCCCCAGCCAACAGAATCAGATTCGGGTGCAGCTAAGCAGCAGCCTTGTGGCCGTTTTTGC
CCAAACGCTGTGCAAGCGCCACAATCCAAGCCCCGGCCAATCTGGTCGAGTCATGGCCCAGGAGATAATGATCAATACAC
CAGCCACCGCAAACCTGATCCGGGAAGGTAAAACCGCCCAGCTTTACTCTGCAATTCAGACAGGTGGTCAACTATCAATG
CAAACGCTGGAAAAAGCCCTTGGGGATCTAGTGCTCAGCAACCAAGTCATGATGGATGAAGCCCTTTTGAAAACCTCAAA
GCCTGACGAGCTCATACGTTTAGTCAAGCAACTGAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

50.432

96.657

0.487
  pilT Pseudomonas stutzeri DSM 10701

49.856

96.657

0.482
  pilT Legionella pneumophila strain Lp02

52.424

91.922

0.482
  pilT Legionella pneumophila strain ERS1305867

52.424

91.922

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.451

98.886

0.479
  pilT Acinetobacter baylyi ADP1

51.988

91.086

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.988

91.086

0.474
  pilT Vibrio cholerae strain A1552

51.988

91.086

0.474
  pilT Acinetobacter baumannii strain A118

48.563

96.936

0.471
  pilT Acinetobacter baumannii D1279779

48.563

96.936

0.471
  pilT Neisseria meningitidis 8013

51.376

91.086

0.468
  pilT Neisseria gonorrhoeae MS11

51.376

91.086

0.468
  pilT Acinetobacter nosocomialis M2

51.07

91.086

0.465
  pilU Acinetobacter baylyi ADP1

38.268

99.721

0.382
  pilU Pseudomonas stutzeri DSM 10701

38.352

98.05

0.376

Multiple sequence alignment