Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   U9970_RS12475 Genome accession   NZ_OY986431
Coordinates   2300800..2302629 (+) Length   609 a.a.
NCBI ID   WP_322764452.1    Uniprot ID   -
Organism   Cyanobium usitatum str. Tous isolate KAC_108     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2295800..2307629
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U9970_RS12445 (OGCDGJMD_02540) murB 2296315..2297226 (+) 912 WP_322764447.1 UDP-N-acetylmuramate dehydrogenase -
  U9970_RS12450 (OGCDGJMD_02541) - 2297262..2297603 (+) 342 WP_254960571.1 YbaB/EbfC family nucleoid-associated protein -
  U9970_RS12455 (OGCDGJMD_02542) rsgA 2297578..2298675 (-) 1098 WP_322764448.1 ribosome small subunit-dependent GTPase A -
  U9970_RS12460 (OGCDGJMD_02543) - 2298665..2298925 (-) 261 WP_322764449.1 sulfurtransferase TusA family protein -
  U9970_RS12465 (OGCDGJMD_02544) dnaJ 2298922..2300037 (-) 1116 WP_322764450.1 molecular chaperone DnaJ -
  U9970_RS12470 (OGCDGJMD_02545) grpE 2300037..2300711 (-) 675 WP_322764451.1 nucleotide exchange factor GrpE -
  U9970_RS12475 (OGCDGJMD_02546) pilB/pilB1 2300800..2302629 (+) 1830 WP_322764452.1 GspE/PulE family protein Machinery gene
  U9970_RS12480 (OGCDGJMD_02547) pilT 2302637..2303716 (+) 1080 WP_254941691.1 type IV pilus twitching motility protein PilT Machinery gene
  U9970_RS12485 (OGCDGJMD_02548) - 2303741..2305000 (+) 1260 WP_254941692.1 type II secretion system F family protein -
  U9970_RS12490 (OGCDGJMD_02549) - 2304988..2306256 (-) 1269 WP_322764453.1 NAD(P)/FAD-dependent oxidoreductase -
  U9970_RS12495 (OGCDGJMD_02550) dusA 2306242..2307288 (+) 1047 WP_322764454.1 tRNA dihydrouridine(20/20a) synthase DusA -

Sequence

Protein


Download         Length: 609 a.a.        Molecular weight: 66353.87 Da        Isoelectric Point: 5.9296

>NTDB_id=1162489 U9970_RS12475 WP_322764452.1 2300800..2302629(+) (pilB/pilB1) [Cyanobium usitatum str. Tous isolate KAC_108]
MTLIPSRPVPEASSEEQQRLEVELLVGEPVLQATELQALGQDLLFTGKGLPEARWREFGALPLQESKGRLVVAVPSHWGP
QQRQNLIEELQRAGFNAELKLALATNLTSALSAKAPTPATAASKPGQPRPPASFTPPRSLTKDLGIPGTSEGQLQEVADV
EDLEGTAAAANSSANASPIISLVDRILIEALTLGASDIHVEPQENTLEIRFRLDGVLQKHFEDLPKSLTPAVTSRIKIMA
DLDIAERRLPQDGRIRRMFRGRKMDFRVSTLPSRNGEKICLRLLDSGATQLGLETLITDADARKTLRELGSKPYGMILVT
GPTGSGKSTTLYALLAERNDPGINISTVEDPIEYTLHGITQTQVNREKGLDFSMALRAFMRQDPDVLLVGETRDLETAKT
AIEAALTGHLVMTTLHCNDAASAIARLDEMGVEPFMVSASLLGIVSQRLLRRVCSACRISYTPNPEELARFGLLSSHETT
VTFFKANTQNRNQEGCCPACNGAGYKGRIGVYEILRMTDTLAAAVAKREPTDVLRRMALENGMKTLLGYGLQLVREGHTT
LAEVERMLLTDSGLEAERRSKSLSTLTCSNCGAGLHDDWLECPYCLTSR

Nucleotide


Download         Length: 1830 bp        

>NTDB_id=1162489 U9970_RS12475 WP_322764452.1 2300800..2302629(+) (pilB/pilB1) [Cyanobium usitatum str. Tous isolate KAC_108]
ATGACCCTCATCCCTTCCCGCCCCGTCCCTGAAGCCAGCAGCGAAGAGCAGCAGCGTCTGGAGGTGGAGCTACTGGTTGG
TGAACCGGTGCTGCAAGCCACCGAGTTGCAGGCCCTTGGTCAAGACTTACTTTTTACGGGCAAAGGGCTGCCAGAGGCCC
GCTGGCGCGAATTTGGCGCCTTGCCCCTTCAAGAAAGCAAAGGGAGATTGGTAGTTGCCGTACCTAGCCACTGGGGGCCC
CAGCAGCGCCAGAACTTAATTGAGGAATTGCAACGTGCCGGCTTCAACGCGGAGCTGAAGTTGGCGTTGGCGACAAATCT
CACATCTGCGCTCTCGGCAAAAGCACCAACTCCCGCTACCGCAGCCTCCAAGCCAGGCCAACCGCGACCACCTGCAAGCT
TTACCCCCCCCCGCTCCCTAACCAAAGACCTAGGAATCCCTGGAACCTCCGAAGGGCAGCTGCAGGAGGTTGCCGATGTC
GAAGATCTGGAGGGCACTGCTGCTGCTGCCAACAGCAGTGCCAATGCGTCACCAATCATCAGCCTGGTGGACAGAATCCT
GATCGAGGCCCTCACCCTGGGGGCCAGTGATATTCACGTGGAACCCCAGGAAAACACCCTGGAAATTCGCTTTCGCCTCG
ACGGTGTGCTGCAGAAGCACTTCGAGGACCTGCCAAAGAGCCTGACGCCTGCCGTCACATCACGCATCAAAATCATGGCG
GATCTGGACATCGCCGAGCGACGATTGCCTCAAGACGGCCGCATCAGGCGCATGTTCCGCGGCCGCAAAATGGATTTTCG
GGTAAGCACTTTACCCAGCCGTAATGGTGAAAAGATATGCCTGCGCCTACTTGACAGCGGCGCTACTCAGCTCGGCCTAG
AAACCCTAATCACCGATGCGGATGCCAGAAAAACCCTGCGAGAGCTGGGATCTAAACCCTACGGAATGATTCTGGTGACG
GGCCCAACCGGCTCGGGCAAGTCAACCACCCTCTACGCCCTACTAGCTGAGCGCAACGATCCAGGTATCAACATTTCCAC
GGTTGAAGACCCGATCGAATACACCCTGCATGGCATCACCCAAACCCAAGTAAATCGAGAGAAGGGGCTCGATTTCAGTA
TGGCGCTACGTGCCTTTATGCGACAGGACCCTGACGTTTTGCTGGTGGGAGAAACCCGCGACTTGGAGACCGCCAAAACG
GCGATCGAGGCCGCACTTACTGGCCACCTAGTGATGACCACCCTGCACTGCAACGACGCGGCCAGCGCCATCGCCCGCCT
CGATGAAATGGGTGTGGAGCCCTTCATGGTGAGCGCATCACTGCTGGGCATCGTGTCCCAACGGCTGCTGCGAAGGGTGT
GTTCGGCTTGCCGAATTAGTTACACCCCCAATCCGGAAGAACTGGCCCGTTTTGGTCTGTTGAGTAGCCACGAAACCACG
GTGACCTTCTTCAAGGCCAACACCCAGAACCGCAACCAGGAAGGCTGCTGCCCTGCATGCAACGGTGCCGGCTACAAAGG
AAGAATCGGTGTCTACGAAATCCTACGGATGACGGATACCTTGGCAGCAGCTGTCGCCAAAAGGGAACCCACAGATGTGC
TGCGGCGAATGGCGCTTGAAAACGGCATGAAAACCCTTTTGGGCTACGGGCTGCAGCTAGTGCGCGAAGGTCACACAACC
CTGGCCGAGGTCGAACGGATGCTGCTCACTGATTCAGGCCTGGAAGCTGAACGCCGCTCTAAATCACTAAGCACCCTCAC
CTGCAGCAACTGCGGAGCTGGGCTCCATGACGACTGGCTCGAATGTCCCTACTGTCTAACCAGCCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(186-452)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

52.597

100

0.532

Multiple sequence alignment