Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   U9971_RS10790 Genome accession   NZ_OY986430
Coordinates   1983429..1984511 (+) Length   360 a.a.
NCBI ID   WP_322771663.1    Uniprot ID   -
Organism   Synechococcus sp. CBW1107 isolate KAC_114     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1978429..1989511
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  U9971_RS10770 (MNNICLKF_02180) - 1979353..1979598 (-) 246 WP_322771659.1 sulfurtransferase TusA family protein -
  U9971_RS10775 (MNNICLKF_02181) dnaJ 1979606..1980733 (-) 1128 WP_322771660.1 molecular chaperone DnaJ -
  U9971_RS10780 (MNNICLKF_02182) grpE 1980737..1981417 (-) 681 WP_322771661.1 nucleotide exchange factor GrpE -
  U9971_RS10785 (MNNICLKF_02183) - 1981498..1983345 (+) 1848 WP_322771662.1 GspE/PulE family protein -
  U9971_RS10790 (MNNICLKF_02184) pilT 1983429..1984511 (+) 1083 WP_322771663.1 type IV pilus twitching motility protein PilT Machinery gene
  U9971_RS10795 (MNNICLKF_02185) - 1984526..1985773 (+) 1248 WP_322771664.1 type II secretion system F family protein -
  U9971_RS10800 (MNNICLKF_02186) - 1985785..1987095 (-) 1311 WP_322771665.1 NAD(P)/FAD-dependent oxidoreductase -
  U9971_RS10805 (MNNICLKF_02187) dusA 1987121..1988119 (+) 999 WP_322771666.1 tRNA dihydrouridine(20/20a) synthase DusA -
  U9971_RS10810 (MNNICLKF_02188) - 1988160..1988654 (-) 495 WP_322771667.1 RNA-binding protein -

Sequence

Protein


Download         Length: 360 a.a.        Molecular weight: 39634.11 Da        Isoelectric Point: 5.9962

>NTDB_id=1162476 U9971_RS10790 WP_322771663.1 1983429..1984511(+) (pilT) [Synechococcus sp. CBW1107 isolate KAC_114]
MELQIEQLMEELVQGGGSDLHLAAGQPPYGRFSGALRPMREEKLSEDQCNRLIFSMINNAQRKQLEQTWELDCAYGLRGV
SRFRVNVYRQRGSYAACLRALGSSIPSLESLELPPVVEETSRRPRGLILVTGPTGSGKTTTLAALLDHINRSRAEHILTI
EDPIEFTYRNEQSVIHQRQLGEDTRSFAAALRAALREDPDVILVGEMRDLETIQLAITAAETGHLVFGTLHTSSAAQTVD
RMVDVFPAEQQTQIRVQLSSSLVGVFSQTLCKRLNPMPGQFGRVMAQEILINTPAIANLIREGKTAQLYSQIQTGGQQGM
QTLERALANLVVQGAVSREEAMAKASKPEELGRLLDGQSG

Nucleotide


Download         Length: 1083 bp        

>NTDB_id=1162476 U9971_RS10790 WP_322771663.1 1983429..1984511(+) (pilT) [Synechococcus sp. CBW1107 isolate KAC_114]
ATGGAGCTCCAGATCGAGCAGCTGATGGAGGAGCTGGTGCAAGGCGGCGGCAGCGATCTGCACCTGGCCGCCGGCCAGCC
CCCCTACGGCCGTTTCAGCGGGGCGCTGCGGCCGATGCGCGAGGAGAAGCTCAGCGAAGACCAGTGCAACCGCCTGATCT
TCTCGATGATCAACAACGCCCAGCGCAAGCAGCTGGAGCAAACCTGGGAGCTCGACTGCGCCTATGGCCTGCGGGGCGTG
TCGCGCTTCCGGGTGAATGTGTACCGCCAACGGGGCAGCTACGCCGCCTGCCTGCGGGCCCTGGGCAGCTCAATTCCCAG
CCTGGAGAGCCTGGAGCTGCCGCCGGTGGTGGAGGAAACCAGCCGCCGACCGCGGGGGCTGATCCTGGTGACCGGGCCCA
CGGGATCGGGCAAAACCACCACCCTGGCGGCACTACTGGATCACATCAACCGCAGCCGCGCCGAGCACATCCTCACGATC
GAAGACCCGATCGAATTCACTTACCGCAACGAGCAGAGCGTGATCCACCAGCGCCAGCTCGGGGAAGACACCCGCAGCTT
CGCTGCTGCCCTGCGGGCGGCCCTGCGCGAAGACCCCGACGTGATCCTGGTGGGGGAGATGCGCGACCTGGAGACGATCC
AGCTGGCGATCACCGCAGCGGAAACAGGCCACCTGGTGTTTGGCACATTGCACACCAGCTCCGCCGCCCAGACGGTGGAT
CGCATGGTGGATGTGTTCCCGGCTGAGCAGCAGACCCAGATCCGCGTGCAGCTGAGCAGTTCGCTGGTGGGGGTGTTCTC
GCAGACCCTGTGCAAGCGGCTGAACCCCATGCCGGGCCAGTTCGGGCGCGTGATGGCCCAGGAGATCCTGATCAACACGC
CGGCCATCGCCAACCTGATCCGCGAGGGCAAAACGGCGCAGCTCTATTCCCAGATCCAAACCGGCGGCCAGCAGGGCATG
CAGACCCTGGAACGGGCCCTGGCCAACCTGGTGGTGCAGGGGGCTGTGAGCCGCGAGGAGGCGATGGCCAAGGCCAGCAA
ACCCGAGGAGCTGGGCCGCCTGCTCGACGGCCAGAGCGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-253)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

53.009

96.944

0.514
  pilT Pseudomonas stutzeri DSM 10701

51.576

96.944

0.5
  pilT Acinetobacter baylyi ADP1

50.716

96.944

0.492
  pilT Acinetobacter baumannii strain A118

50.43

96.944

0.489
  pilT Acinetobacter baumannii D1279779

50.43

96.944

0.489
  pilT Acinetobacter nosocomialis M2

50.43

96.944

0.489
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.02

99.167

0.486
  pilT Neisseria meningitidis 8013

49.57

96.944

0.481
  pilT Legionella pneumophila strain ERS1305867

51.032

94.167

0.481
  pilT Legionella pneumophila strain Lp02

51.032

94.167

0.481
  pilT Neisseria gonorrhoeae MS11

49.284

96.944

0.478
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.292

95

0.478
  pilT Vibrio cholerae strain A1552

50.292

95

0.478
  pilU Vibrio cholerae strain A1552

37.989

99.444

0.378
  pilU Pseudomonas stutzeri DSM 10701

38.968

96.944

0.378
  pilU Acinetobacter baylyi ADP1

37.714

97.222

0.367

Multiple sequence alignment