Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   R2K28_RS19560 Genome accession   NZ_OY734020
Coordinates   4276896..4278005 (+) Length   369 a.a.
NCBI ID   WP_116446337.1    Uniprot ID   -
Organism   Candidatus Thiodiazotropha sp. CDECU1 isolate 46184     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4271896..4283005
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R2K28_RS19530 argH 4272534..4273928 (+) 1395 WP_316367111.1 argininosuccinate lyase -
  R2K28_RS19535 - 4273936..4274301 (+) 366 WP_116446332.1 VOC family protein -
  R2K28_RS19540 - 4274349..4274684 (-) 336 WP_316367114.1 hypothetical protein -
  R2K28_RS19545 - 4274844..4275797 (+) 954 WP_316367116.1 zinc metalloprotease HtpX -
  R2K28_RS19550 - 4275833..4276192 (+) 360 WP_116446335.1 nucleotide pyrophosphohydrolase -
  R2K28_RS19555 - 4276313..4276483 (+) 171 WP_316367118.1 rubredoxin -
  R2K28_RS19560 pilU 4276896..4278005 (+) 1110 WP_116446337.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R2K28_RS19565 - 4277996..4278556 (-) 561 WP_316367121.1 antibiotic biosynthesis monooxygenase -
  R2K28_RS19570 - 4278569..4279213 (-) 645 WP_316367123.1 glutathione S-transferase family protein -
  R2K28_RS19575 - 4279210..4279806 (-) 597 WP_316367125.1 2-hydroxychromene-2-carboxylate isomerase -
  R2K28_RS19580 - 4279871..4281181 (-) 1311 WP_316367128.1 DUF1329 domain-containing protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41193.28 Da        Isoelectric Point: 6.3714

>NTDB_id=1160764 R2K28_RS19560 WP_116446337.1 4276896..4278005(+) (pilU) [Candidatus Thiodiazotropha sp. CDECU1 isolate 46184]
MDIVPYLKLMVQKNGSDLFFSTGAAPHLKTEGETRPIGSSPMQSGQVRKLAYGIMNDDQIKEYEATYECNLAISVNQLGR
FRVNVYKQRGDAAMVIRYIKGVIPPVEKLNLPIILKDLIMEPRGLILVVGATGSGKSTTLAAMIEHRNQNVAGHILTIED
PIEYLYTHKKSVVDQREVGLDTLSYENALKNAMREAPDVIQIGEIREMHTMQHAIAYAETGHLCLSTLHANNSNQALDRI
INFFPDTAHHQLYMDLSLNLRAVISQRLVKGINGQRIPAVEIMLLSTYISELIQKGDIHTIKEVMEQGTERGMQTFDQSL
YKLYKEGKISMEEALSHADSRNNLSLKIRLSDTEGVEAPGSLGVAEDQF

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1160764 R2K28_RS19560 WP_116446337.1 4276896..4278005(+) (pilU) [Candidatus Thiodiazotropha sp. CDECU1 isolate 46184]
ATGGATATCGTGCCCTATCTGAAGCTTATGGTGCAGAAAAATGGCTCAGATCTCTTTTTCAGCACCGGTGCCGCCCCCCA
TTTGAAAACCGAGGGTGAAACCCGCCCGATAGGGTCCTCCCCGATGCAAAGCGGACAGGTCAGAAAGCTCGCTTACGGCA
TCATGAACGACGATCAGATCAAGGAGTACGAAGCCACTTACGAGTGTAATCTGGCTATCTCGGTCAACCAGCTGGGCCGC
TTCCGCGTCAATGTCTATAAACAGCGGGGTGATGCGGCGATGGTGATTCGCTATATCAAGGGCGTGATCCCCCCCGTAGA
GAAGCTCAATCTGCCGATTATCCTCAAGGACCTGATCATGGAACCCCGCGGATTGATCCTGGTGGTTGGGGCAACCGGTT
CAGGTAAATCGACTACCCTGGCAGCGATGATCGAGCACCGCAATCAAAATGTTGCCGGGCATATATTAACCATCGAGGAT
CCCATCGAATATCTCTACACGCATAAAAAATCCGTGGTCGATCAACGCGAGGTGGGACTCGACACACTCTCCTATGAAAA
TGCCCTGAAGAATGCCATGCGCGAGGCGCCGGATGTGATACAGATCGGCGAAATCCGTGAAATGCACACCATGCAGCACG
CGATCGCCTACGCGGAAACCGGCCATCTGTGCCTATCGACCCTGCATGCCAACAACTCAAACCAGGCCCTTGACCGCATC
ATCAACTTCTTTCCCGACACAGCCCATCATCAGCTCTACATGGATCTTTCCCTCAACCTCCGTGCAGTCATATCGCAACG
TCTGGTAAAGGGTATCAACGGCCAGCGCATCCCCGCGGTCGAGATCATGCTGCTGTCGACCTACATCTCAGAACTGATTC
AGAAAGGGGATATTCACACCATAAAAGAGGTCATGGAACAGGGTACCGAACGGGGTATGCAGACCTTCGATCAATCCCTC
TACAAACTCTACAAAGAGGGTAAGATCTCCATGGAAGAGGCGCTCTCCCATGCTGACTCGCGCAATAACCTGTCACTCAA
GATACGCCTCTCCGACACCGAAGGTGTCGAAGCCCCGGGCAGCCTTGGTGTGGCCGAGGATCAATTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.661

96.748

0.509
  pilU Acinetobacter baylyi ADP1

50.565

95.935

0.485
  pilU Vibrio cholerae strain A1552

48.857

94.851

0.463
  pilT Acinetobacter baylyi ADP1

42.941

92.141

0.396
  pilT Legionella pneumophila strain ERS1305867

43.114

90.515

0.39
  pilT Legionella pneumophila strain Lp02

43.114

90.515

0.39
  pilT Pseudomonas aeruginosa PAK

40.698

93.225

0.379
  pilT Neisseria meningitidis 8013

40.23

94.309

0.379
  pilT Acinetobacter baumannii strain A118

41.42

91.599

0.379
  pilT Acinetobacter nosocomialis M2

41.42

91.599

0.379
  pilT Acinetobacter baumannii D1279779

41.42

91.599

0.379
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.369

91.057

0.377
  pilT Vibrio cholerae strain A1552

41.369

91.057

0.377
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.708

91.87

0.374
  pilT Pseudomonas stutzeri DSM 10701

40.116

93.225

0.374
  pilT Neisseria gonorrhoeae MS11

39.481

94.038

0.371

Multiple sequence alignment