Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QWI75_RS16305 Genome accession   NZ_OX365700
Coordinates   3445583..3446662 (-) Length   359 a.a.
NCBI ID   WP_289269726.1    Uniprot ID   -
Organism   Nitrospira tepida strain DNF     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3440583..3451662
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QWI75_RS16280 (DNFV4_03368) - 3441072..3442772 (+) 1701 WP_289269717.1 PAS domain-containing protein -
  QWI75_RS16285 (DNFV4_03369) - 3442769..3443206 (+) 438 WP_289269719.1 response regulator -
  QWI75_RS16290 (DNFV4_03370) - 3443216..3443635 (+) 420 WP_289269721.1 response regulator -
  QWI75_RS16295 (DNFV4_03371) - 3443639..3444802 (+) 1164 WP_289269723.1 ATP-binding protein -
  QWI75_RS16300 (DNFV4_03372) - 3444885..3445301 (+) 417 WP_289269724.1 response regulator -
  QWI75_RS16305 (DNFV4_03373) pilT 3445583..3446662 (-) 1080 WP_289269726.1 type IV pilus twitching motility protein PilT Machinery gene
  QWI75_RS16310 (DNFV4_03374) - 3446742..3447128 (-) 387 WP_289269728.1 PilZ domain-containing protein -
  QWI75_RS16315 (DNFV4_03375) - 3447125..3450019 (-) 2895 WP_289269730.1 PAS domain S-box protein -
  QWI75_RS16320 (DNFV4_03376) - 3450118..3450489 (-) 372 WP_289269731.1 hypothetical protein -
  QWI75_RS16325 (DNFV4_03377) - 3450486..3450896 (-) 411 WP_289269733.1 response regulator -

Sequence

Protein


Download         Length: 359 a.a.        Molecular weight: 39623.55 Da        Isoelectric Point: 7.1362

>NTDB_id=1155800 QWI75_RS16305 WP_289269726.1 3445583..3446662(-) (pilT) [Nitrospira tepida strain DNF]
MAKIDDLFRMLADRGGSDLHLIAGQRPSMRLHGELERIDGQGALEDGALRELLYEITPAPKRAQFEETGDVDFGYEIPGL
ARFRANLFNQKYGCAAVFRKIPSQVLTADELGLPPILTRAAMLKKGLVLVTGPTGSGKSTTLAAMIDHANKHRRDHILTI
EDPIEFVHQSHGCIVNHREVGVHTQSFAAALRGALREDPDIILVGEMRDLETIRLAVEAASTGHLVFGTLHTESAAKTVD
RVIEVFPHQEQAQIRNTLSTALRVVVAQNLFKRIDQKGRCAALEILVCTPAVANLIRDAKTFQIASVMQTGRNVGMQTLD
DAIQDLLNRKWISPEEAHEKAIDKTRFAKFLKTPPDALQ

Nucleotide


Download         Length: 1080 bp        

>NTDB_id=1155800 QWI75_RS16305 WP_289269726.1 3445583..3446662(-) (pilT) [Nitrospira tepida strain DNF]
ATGGCCAAAATTGATGACCTGTTCCGGATGCTCGCCGATCGCGGAGGATCGGATCTCCACCTGATCGCCGGCCAACGCCC
CTCGATGCGACTCCATGGAGAACTCGAACGGATCGACGGGCAGGGCGCGCTGGAGGATGGTGCTCTGCGTGAACTTCTAT
ACGAGATCACGCCCGCTCCCAAGCGAGCACAATTCGAGGAAACGGGAGACGTCGATTTCGGTTATGAAATCCCGGGACTG
GCGCGTTTCCGGGCCAATCTATTCAATCAGAAGTACGGGTGCGCAGCGGTCTTTCGTAAGATCCCCAGTCAGGTACTCAC
AGCCGACGAACTGGGCCTGCCGCCAATCCTGACCCGCGCGGCGATGCTCAAGAAAGGGTTGGTGCTGGTGACGGGGCCGA
CGGGCAGCGGCAAATCCACGACGCTCGCCGCGATGATCGACCATGCCAACAAGCATCGGCGGGATCACATTCTCACTATC
GAGGACCCGATCGAGTTCGTCCACCAGAGCCACGGCTGCATCGTCAATCATCGGGAGGTAGGCGTCCATACCCAATCGTT
TGCGGCCGCGCTGCGGGGAGCGCTGCGGGAGGACCCGGACATCATCCTGGTGGGAGAAATGCGGGATTTGGAAACGATCC
GGTTAGCGGTCGAGGCAGCCTCGACCGGGCATCTGGTGTTCGGCACGCTCCACACGGAAAGCGCGGCGAAAACCGTCGAC
CGGGTGATCGAAGTGTTTCCCCATCAAGAGCAGGCCCAGATCCGCAACACGCTCTCGACGGCGCTGCGGGTGGTGGTCGC
TCAAAACCTGTTCAAGCGGATTGATCAAAAGGGACGCTGCGCGGCGCTGGAAATCCTGGTGTGCACGCCCGCGGTCGCGA
ACCTGATCCGGGACGCCAAAACCTTTCAGATCGCCTCGGTCATGCAGACCGGGAGGAACGTCGGCATGCAAACGTTGGAT
GACGCGATTCAGGACCTCCTGAACAGGAAATGGATCAGCCCGGAAGAGGCCCATGAGAAGGCGATCGACAAGACCCGGTT
CGCGAAGTTCCTCAAGACTCCCCCGGACGCATTGCAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

56.509

94.15

0.532
  pilT Legionella pneumophila strain ERS1305867

55.523

95.822

0.532
  pilT Legionella pneumophila strain Lp02

55.523

95.822

0.532
  pilT Acinetobacter baumannii strain A118

55.325

94.15

0.521
  pilT Acinetobacter nosocomialis M2

55.325

94.15

0.521
  pilT Acinetobacter baumannii D1279779

55.325

94.15

0.521
  pilT Pseudomonas stutzeri DSM 10701

54.07

95.822

0.518
  pilT Neisseria meningitidis 8013

53.372

94.986

0.507
  pilT Neisseria gonorrhoeae MS11

53.079

94.986

0.504
  pilT Acinetobacter baylyi ADP1

54.848

91.922

0.504
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.143

97.214

0.487
  pilT Vibrio cholerae strain A1552

51.039

93.872

0.479
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.039

93.872

0.479
  pilU Pseudomonas stutzeri DSM 10701

40.597

93.315

0.379
  pilU Vibrio cholerae strain A1552

39.941

94.15

0.376
  pilU Acinetobacter baylyi ADP1

39.823

94.429

0.376

Multiple sequence alignment