Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ONG87_RS01400 Genome accession   NZ_OX352319
Coordinates   309771..310805 (+) Length   344 a.a.
NCBI ID   WP_122480509.1    Uniprot ID   -
Organism   Pseudomonas sp. MM227     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 304771..315805
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ONG87_RS01370 (AHFPHNDE_00271) rdgB 304905..305501 (-) 597 WP_192102823.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -
  ONG87_RS01375 (AHFPHNDE_00272) metW 305614..306234 (-) 621 WP_043191520.1 methionine biosynthesis protein MetW -
  ONG87_RS01380 (AHFPHNDE_00273) - 306242..307381 (-) 1140 WP_122480513.1 homoserine O-acetyltransferase -
  ONG87_RS01385 (AHFPHNDE_00274) - 307534..308124 (-) 591 WP_122480512.1 YggT family protein -
  ONG87_RS01390 (AHFPHNDE_00275) proC 308146..308967 (-) 822 WP_122480511.1 pyrroline-5-carboxylate reductase -
  ONG87_RS01395 (AHFPHNDE_00276) - 308964..309704 (-) 741 WP_264382078.1 YggS family pyridoxal phosphate-dependent enzyme -
  ONG87_RS01400 (AHFPHNDE_00277) pilT 309771..310805 (+) 1035 WP_122480509.1 type IV pilus twitching motility protein PilT Machinery gene
  ONG87_RS01405 (AHFPHNDE_00278) - 310927..311562 (-) 636 WP_122480508.1 C40 family peptidase -
  ONG87_RS01410 (AHFPHNDE_00279) - 311812..312219 (+) 408 WP_122480507.1 TM2 domain-containing protein -
  ONG87_RS01415 (AHFPHNDE_00280) - 312532..313803 (-) 1272 WP_192137550.1 dihydroorotase -
  ONG87_RS01420 (AHFPHNDE_00281) - 313800..314804 (-) 1005 WP_122480505.1 aspartate carbamoyltransferase catalytic subunit -
  ONG87_RS01425 (AHFPHNDE_00282) pyrR 314816..315334 (-) 519 WP_122480504.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37881.52 Da        Isoelectric Point: 6.6651

>NTDB_id=1155272 ONG87_RS01400 WP_122480509.1 309771..310805(+) (pilT) [Pseudomonas sp. MM227]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPALDDKQVQALIYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLSMDDLGMGDVFRKITEVPRGLVLVTGPTGSGKSTTLAAMIDYLNSNKHHHILTVED
PIEFVHESKKCLVNQREVHRDTLGFSHALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
VDVFPAEEKSMVRSMLSESLQAVISQTLLKKIGGGRVAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGGSLGMQTLDMCL
KNLLAQGIISRDSAREKAKSPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1155272 ONG87_RS01400 WP_122480509.1 309771..310805(+) (pilT) [Pseudomonas sp. MM227]
ATGGACATTACCGAGCTGTTGGCCTTCAGTGCGAAACAAGGCGCATCCGACCTGCACCTGTCCGCAGGCCTGCCGCCGAT
GATTCGGGTGGATGGCGACGTGCGCCGTATCAACCTGCCGGCGCTGGACGACAAACAGGTCCAGGCGCTGATCTACGACA
TCATGAACGACAAGCAGCGCAAGGACTTCGAGGAGTTTCTGGAAACCGACTTTTCCTTCGAAGTGCCCGGTGTGGCGCGC
TTTCGGGTCAACGCGTTCAACCAAAACCGCGGTGCCGGCGCAGTGTTTCGAACCATTCCGTCCAAGGTGCTGAGCATGGA
CGACCTGGGCATGGGAGATGTGTTTCGCAAGATTACCGAAGTGCCGCGCGGGCTGGTGCTGGTCACCGGGCCGACCGGGT
CGGGCAAGTCGACTACCCTGGCGGCAATGATCGATTACCTCAACAGCAACAAGCACCATCACATCCTGACCGTGGAAGAT
CCGATCGAATTCGTCCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGTGAAGTGCACCGCGACACCCTGGGGTTCTCCCA
CGCCTTGCGCTCGGCGTTGCGTGAGGACCCGGACGTGATCCTGGTGGGCGAAATGCGCGACCTGGAAACCATTCGGCTGG
CGTTGACGGCGGCCGAGACCGGGCACCTGGTGTTCGGCACGCTGCACACCACGTCGGCGGCGAAGACCATCGACCGGGTG
GTCGATGTGTTTCCGGCAGAGGAAAAGTCGATGGTGCGTTCAATGCTGTCCGAATCGCTGCAGGCAGTGATTTCCCAGAC
CTTGCTGAAGAAGATTGGCGGCGGGCGGGTGGCTGCGCACGAGATCATGATCGGCACCCCGGCGATTCGTAACCTCATCC
GTGAAGACAAGGTCGCGCAGATGTACTCGTCGATCCAGACAGGTGGGTCGCTGGGCATGCAGACCTTGGACATGTGCCTG
AAGAACCTGCTCGCCCAGGGCATCATCAGCCGCGACTCGGCCCGTGAAAAGGCCAAGTCCCCGGACAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

93.314

100

0.933
  pilT Pseudomonas aeruginosa PAK

90.698

100

0.907
  pilT Acinetobacter baumannii D1279779

81.977

100

0.82
  pilT Acinetobacter baumannii strain A118

81.977

100

0.82
  pilT Acinetobacter nosocomialis M2

81.686

100

0.817
  pilT Acinetobacter baylyi ADP1

81.105

100

0.811
  pilT Legionella pneumophila strain Lp02

74.852

98.256

0.735
  pilT Legionella pneumophila strain ERS1305867

74.852

98.256

0.735
  pilT Neisseria meningitidis 8013

66.957

100

0.672
  pilT Neisseria gonorrhoeae MS11

66.667

100

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

67.359

97.965

0.66
  pilT Vibrio cholerae strain A1552

67.359

97.965

0.66
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.523
  pilU Pseudomonas stutzeri DSM 10701

41.159

100

0.413
  pilU Acinetobacter baylyi ADP1

40.751

100

0.41
  pilU Vibrio cholerae strain A1552

40.29

100

0.404

Multiple sequence alignment