Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   N4J75_RS06340 Genome accession   NZ_OX291796
Coordinates   1340776..1341798 (-) Length   340 a.a.
NCBI ID   WP_038879280.1    Uniprot ID   -
Organism   Serratia marcescens strain SJC1050     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1335776..1346798
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4J75_RS06310 (SAMEA13201017_01233) - 1336577..1337089 (+) 513 WP_038879273.1 SprT family zinc-dependent metalloprotease -
  N4J75_RS06315 (SAMEA13201017_01234) endA 1337191..1337886 (+) 696 WP_038879275.1 deoxyribonuclease I -
  N4J75_RS06320 (SAMEA13201017_01235) rsmE 1337956..1338687 (+) 732 WP_033635938.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  N4J75_RS06325 (SAMEA13201017_01236) gshB 1338698..1339648 (+) 951 WP_038879277.1 glutathione synthase -
  N4J75_RS06330 (SAMEA13201017_01237) - 1339794..1340357 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  N4J75_RS06335 (SAMEA13201017_01238) ruvX 1340357..1340779 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  N4J75_RS06340 (SAMEA13201017_01239) pilT 1340776..1341798 (-) 1023 WP_038879280.1 type IV pilus twitching motility protein PilT Machinery gene
  N4J75_RS06345 (SAMEA13201017_01240) - 1341819..1342526 (+) 708 WP_038879282.1 YggS family pyridoxal phosphate-dependent enzyme -
  N4J75_RS06350 (SAMEA13201017_01241) proC 1342546..1343367 (+) 822 WP_038879285.1 pyrroline-5-carboxylate reductase -
  N4J75_RS06355 (SAMEA13201017_01242) - 1343399..1343953 (+) 555 WP_033635944.1 YggT family protein -
  N4J75_RS06360 (SAMEA13201017_01243) yggU 1343950..1344240 (+) 291 WP_033649585.1 DUF167 family protein YggU -
  N4J75_RS06365 (SAMEA13201017_01244) - 1344258..1344851 (+) 594 WP_038879287.1 XTP/dITP diphosphatase -
  N4J75_RS06370 (SAMEA13201017_01245) hemW 1344844..1345986 (+) 1143 WP_038879290.1 radical SAM family heme chaperone HemW -
  N4J75_RS06375 (SAMEA13201017_01246) - 1346023..1346271 (-) 249 WP_038879292.1 DUF2442 domain-containing protein -
  N4J75_RS06380 (SAMEA13201017_01247) - 1346255..1346497 (-) 243 WP_004937418.1 DUF4160 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36492.82 Da        Isoelectric Point: 6.8839

>NTDB_id=1154633 N4J75_RS06340 WP_038879280.1 1340776..1341798(-) (pilT) [Serratia marcescens strain SJC1050]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQTLCDGLLNAQQRESLRRLGQIDLALHRPGGE
RLRANVFQQSAGMSLALRRIAGQAPSLAELALPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDIATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKTYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGDCVGEGAGD

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=1154633 N4J75_RS06340 WP_038879280.1 1340776..1341798(-) (pilT) [Serratia marcescens strain SJC1050]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGACCCTCTGTGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGATCGATCTGGCGCTGCACAGGCCGGGTGGGGAG
CGGCTGCGAGCCAACGTTTTTCAGCAAAGCGCGGGGATGTCTCTCGCATTGCGGCGCATCGCCGGGCAGGCGCCTTCGCT
TGCCGAGCTGGCGCTGCCGGCCATCGTCCCGGCACTGCTGCGGCGCGACGACGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACATTTTAACGCTGGAA
GATCCGATCGAATTCCTGCACCGCAGCCGGCGATCGCTGATTCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGTGAGCTGCGCGATATCGCCACCATTCGGT
TGGCGCTCACTGCGGCGGAGACCGGCCATCTGGTGCTGGCGACGTTGCACACCCGCAGCGCGCCGCAGGCGGTGGAAAGG
TTGGTGGACGTGTTTCCGGCGGAAGAGAAAACCTATGTGCGTGCCCAGTTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGCGGCGGCCCGGCGGCGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGAAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGC
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGATTGTGTTGGCGAAGGGGCGGGGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(68-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.242

97.059

0.526
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.242

97.059

0.526
  pilT Acinetobacter baylyi ADP1

51.662

97.353

0.503
  pilT Acinetobacter baumannii D1279779

51.212

97.059

0.497
  pilT Acinetobacter baumannii strain A118

51.212

97.059

0.497
  pilT Acinetobacter nosocomialis M2

51.212

97.059

0.497
  pilT Neisseria meningitidis 8013

49.702

98.824

0.491
  pilT Neisseria gonorrhoeae MS11

49.699

97.647

0.485
  pilT Legionella pneumophila strain ERS1305867

49.849

97.353

0.485
  pilT Legionella pneumophila strain Lp02

49.849

97.353

0.485
  pilT Pseudomonas aeruginosa PAK

49.394

97.059

0.479
  pilT Pseudomonas stutzeri DSM 10701

48.788

97.059

0.474
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.322

100

0.456
  pilU Acinetobacter baylyi ADP1

40.248

95

0.382
  pilU Vibrio cholerae strain A1552

40.123

95.294

0.382
  pilU Pseudomonas stutzeri DSM 10701

39.198

95.294

0.374

Multiple sequence alignment