Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LQV24_RS03680 Genome accession   NZ_OW969711
Coordinates   773713..774693 (+) Length   326 a.a.
NCBI ID   WP_110493835.1    Uniprot ID   -
Organism   Citrobacter koseri isolate 0     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 768713..779693
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LQV24_RS03650 (AN2353V1_0719) ansB 769224..770270 (+) 1047 WP_049010789.1 L-asparaginase 2 -
  LQV24_RS03655 (AN2353V1_0720) hemW 770389..771525 (-) 1137 WP_230009967.1 radical SAM family heme chaperone HemW -
  LQV24_RS03660 (AN2353V1_0721) - 771518..772111 (-) 594 WP_012135070.1 XTP/dITP diphosphatase -
  LQV24_RS03665 (AN2353V1_0722) yggU 772119..772409 (-) 291 WP_012135069.1 DUF167 family protein YggU -
  LQV24_RS03670 (AN2353V1_0723) - 772406..772972 (-) 567 WP_012135068.1 YggT family protein -
  LQV24_RS03675 (AN2353V1_0724) - 772991..773695 (-) 705 WP_049010785.1 YggS family pyridoxal phosphate-dependent enzyme -
  LQV24_RS03680 (AN2353V1_0725) pilT 773713..774693 (+) 981 WP_110493835.1 type IV pilus twitching motility protein PilT Machinery gene
  LQV24_RS03685 (AN2353V1_0726) ruvX 774787..775203 (-) 417 WP_071257864.1 Holliday junction resolvase RuvX -
  LQV24_RS03690 (AN2353V1_0727) - 775203..775766 (-) 564 WP_012135063.1 YqgE/AlgH family protein -
  LQV24_RS03695 (AN2353V1_0728) gshB 775943..776890 (-) 948 WP_230009970.1 glutathione synthase -
  LQV24_RS03700 (AN2353V1_0729) rsmE 776903..777634 (-) 732 WP_049010798.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LQV24_RS03705 (AN2353V1_0730) endA 777712..778419 (-) 708 WP_012135060.1 deoxyribonuclease I -
  LQV24_RS03710 (AN2353V1_0731) - 778514..779011 (-) 498 WP_058668076.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35667.65 Da        Isoelectric Point: 6.8793

>NTDB_id=1153631 LQV24_RS03680 WP_110493835.1 773713..774693(+) (pilT) [Citrobacter koseri isolate 0]
MDMEEIVALSVKHNVSDLHLCNAWPARWRKRGKVEIAPFSSPNVEVLLSQWLNDAQRRAWQADGQLDFAVSLPGELRLRA
SAFHHEQGASLALRLLPTTCPQLDALQTPAALPALLGGENGLILVTGATGSGKSTTLAAMVEYLNQHLDGHILTLEDPIE
YRHTSQRCLIQQREVGVHCRSFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRSQLAGSLRAVLSQKLETDRQDGRVALFELLINTPAVGNLIREGKIHQLPGVIQTSQQAGMQTFAQSQQQR
QAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1153631 LQV24_RS03680 WP_110493835.1 773713..774693(+) (pilT) [Citrobacter koseri isolate 0]
ATGGATATGGAAGAAATAGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAACGCATGGCCTGCACG
CTGGCGTAAACGGGGAAAAGTGGAAATAGCCCCGTTTAGCAGCCCGAATGTCGAGGTTTTGCTGTCACAATGGCTTAACG
ACGCGCAGCGCCGGGCGTGGCAGGCGGATGGTCAGCTCGACTTTGCGGTGTCGCTGCCGGGCGAACTGCGGTTACGCGCC
AGCGCTTTTCATCATGAGCAGGGCGCATCGCTGGCGCTGCGGTTGCTGCCCACTACGTGCCCGCAACTGGACGCGCTCCA
GACGCCTGCCGCGCTACCCGCGCTCCTTGGCGGCGAGAACGGACTGATTCTGGTGACCGGGGCGACGGGAAGCGGGAAAT
CCACCACGCTGGCGGCAATGGTGGAATACCTCAACCAGCATCTCGATGGTCATATTCTGACGCTGGAAGATCCTATTGAG
TACCGCCATACCAGCCAGCGCTGCCTGATTCAGCAGCGGGAGGTTGGCGTACACTGCCGCTCCTTTGCCGCCGGGTTACG
CGCTGCGCTGCGTGAAGACCCGGATGTGATTTTATTAGGCGAACTGCGCGACAGCGAGACTATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCGACGCTACATACGCGCGGCGCGGCACAGGCGGTAGAGCGGCTGGTTGACTCC
TTTCCGGCGCAGGAGAAAGATCCGGTACGCAGTCAACTTGCGGGCAGCCTGCGCGCCGTGTTGTCGCAGAAGCTGGAAAC
CGACAGGCAGGACGGACGCGTGGCGTTATTCGAACTGCTCATCAACACGCCAGCGGTTGGCAATCTTATTCGGGAGGGGA
AAATTCACCAGTTGCCCGGCGTTATTCAGACCAGCCAGCAGGCGGGGATGCAGACGTTTGCCCAGAGTCAGCAACAGCGG
CAGGCGCAGGGCAGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(52-240)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

51.07

100

0.512
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

51.07

100

0.512
  pilT Neisseria meningitidis 8013

49.085

100

0.494
  pilT Neisseria gonorrhoeae MS11

48.78

100

0.491
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Pseudomonas stutzeri DSM 10701

46.789

100

0.469
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Acinetobacter baumannii strain A118

46.483

100

0.466
  pilT Acinetobacter baumannii D1279779

46.483

100

0.466
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Legionella pneumophila strain ERS1305867

46.855

97.546

0.457
  pilT Legionella pneumophila strain Lp02

46.855

97.546

0.457
  pilU Pseudomonas stutzeri DSM 10701

40.606

100

0.411
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

38.39

99.08

0.38

Multiple sequence alignment