Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LQ102_RS20815 Genome accession   NZ_OW967830
Coordinates   4388952..4389974 (-) Length   340 a.a.
NCBI ID   WP_174158604.1    Uniprot ID   -
Organism   Serratia marcescens isolate 0     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4383952..4394974
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LQ102_RS20785 (AI2872V1_3985) - 4384756..4385268 (+) 513 WP_174158605.1 SprT family zinc-dependent metalloprotease -
  LQ102_RS20790 (AI2872V1_3986) endA 4385370..4386065 (+) 696 WP_041036666.1 deoxyribonuclease I -
  LQ102_RS20795 (AI2872V1_3987) rsmE 4386135..4386866 (+) 732 WP_033654665.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LQ102_RS20800 (AI2872V1_3988) gshB 4386877..4387827 (+) 951 WP_033649589.1 glutathione synthase -
  LQ102_RS20805 (AI2872V1_3989) - 4387970..4388533 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  LQ102_RS20810 (AI2872V1_3990) ruvX 4388533..4388955 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  LQ102_RS20815 (AI2872V1_3991) pilT 4388952..4389974 (-) 1023 WP_174158604.1 type IV pilus twitching motility protein PilT Machinery gene
  LQ102_RS20820 (AI2872V1_3992) - 4389995..4390702 (+) 708 WP_033649587.1 YggS family pyridoxal phosphate-dependent enzyme -
  LQ102_RS20825 (AI2872V1_3993) proC 4390722..4391543 (+) 822 WP_049213489.1 pyrroline-5-carboxylate reductase -
  LQ102_RS20830 (AI2872V1_3994) - 4391575..4392129 (+) 555 WP_033635944.1 YggT family protein -
  LQ102_RS20835 (AI2872V1_3995) yggU 4392126..4392416 (+) 291 WP_033649585.1 DUF167 family protein YggU -
  LQ102_RS20840 (AI2872V1_3996) - 4392434..4393027 (+) 594 WP_033635946.1 XTP/dITP diphosphatase -
  LQ102_RS20845 (AI2872V1_3997) hemW 4393020..4394162 (+) 1143 WP_033635947.1 radical SAM family heme chaperone HemW -
  LQ102_RS20850 - 4394199..4394285 (-) 87 Protein_4071 DUF2442 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36406.67 Da        Isoelectric Point: 7.0289

>NTDB_id=1153176 LQ102_RS20815 WP_174158604.1 4388952..4389974(-) (pilT) [Serratia marcescens isolate 0]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANVFQQSAGMSLALRRIAGQAPSLAELAAPAIVPALLRRDNGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDSQSFDAALRAALREDPDVILLGELRDIATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGESAGEGTEG

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=1153176 LQ102_RS20815 WP_174158604.1 4388952..4389974(-) (pilT) [Serratia marcescens isolate 0]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCTTGCGGCGGCTGGGGCAGGTCGATCTGGCGCTGCACCGGCCGGGCGGGGAA
CGGCTGCGGGCCAACGTTTTTCAGCAAAGCGCGGGGATGTCTCTCGCGTTGCGGCGTATCGCCGGGCAGGCGCCTTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGACAACGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGACACCAGCCGCGGCACATTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGTTCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGTCAAAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGCGAACTGCGCGATATTGCCACCATTCGGC
TGGCGCTCACCGCGGCGGAGACCGGCCACCTGGTGCTGGCGACGCTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGCGGCGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGAGTGCGGGAGAGGGAACGGAAGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

51.36

97.353

0.5
  pilT Neisseria gonorrhoeae MS11

50.149

98.529

0.494
  pilT Neisseria meningitidis 8013

50.755

97.353

0.494
  pilT Acinetobacter nosocomialis M2

50.606

97.059

0.491
  pilT Acinetobacter baumannii D1279779

50.606

97.059

0.491
  pilT Acinetobacter baumannii strain A118

50.606

97.059

0.491
  pilT Pseudomonas stutzeri DSM 10701

48.961

99.118

0.485
  pilT Legionella pneumophila strain ERS1305867

49.398

97.647

0.482
  pilT Legionella pneumophila strain Lp02

49.398

97.647

0.482
  pilT Pseudomonas aeruginosa PAK

49.697

97.059

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.82

100

0.459
  pilU Vibrio cholerae strain A1552

39.877

95.882

0.382
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.889

95.294

0.371

Multiple sequence alignment