Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LQ109_RS20890 Genome accession   NZ_OW849139
Coordinates   4365500..4366522 (-) Length   340 a.a.
NCBI ID   WP_060430992.1    Uniprot ID   -
Organism   Serratia marcescens isolate 0     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4360500..4371522
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LQ109_RS20860 (AI2795V1_3998) - 4361301..4361813 (+) 513 WP_060429432.1 SprT family zinc-dependent metalloprotease -
  LQ109_RS20865 (AI2795V1_3999) endA 4361915..4362610 (+) 696 WP_033649591.1 deoxyribonuclease I -
  LQ109_RS20870 (AI2795V1_4000) rsmE 4362680..4363411 (+) 732 WP_060430995.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LQ109_RS20875 (AI2795V1_4001) gshB 4363422..4364372 (+) 951 WP_047729854.1 glutathione synthase -
  LQ109_RS20880 (AI2795V1_4002) - 4364518..4365081 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  LQ109_RS20885 (AI2795V1_4003) ruvX 4365081..4365503 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  LQ109_RS20890 (AI2795V1_4004) pilT 4365500..4366522 (-) 1023 WP_060430992.1 type IV pilus twitching motility protein PilT Machinery gene
  LQ109_RS20895 (AI2795V1_4005) - 4366543..4367250 (+) 708 WP_060426992.1 YggS family pyridoxal phosphate-dependent enzyme -
  LQ109_RS20900 (AI2795V1_4006) proC 4367270..4368091 (+) 822 WP_033649586.1 pyrroline-5-carboxylate reductase -
  LQ109_RS20905 (AI2795V1_4007) - 4368123..4368677 (+) 555 WP_033635944.1 YggT family protein -
  LQ109_RS20910 (AI2795V1_4008) yggU 4368674..4368964 (+) 291 WP_004948014.1 DUF167 family protein YggU -
  LQ109_RS20915 (AI2795V1_4009) - 4368982..4369575 (+) 594 WP_060388227.1 XTP/dITP diphosphatase -
  LQ109_RS20920 (AI2795V1_4010) hemW 4369568..4370710 (+) 1143 WP_060388228.1 radical SAM family heme chaperone HemW -
  LQ109_RS20925 - 4370747..4370833 (-) 87 Protein_4090 DUF2442 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36444.72 Da        Isoelectric Point: 6.8881

>NTDB_id=1152830 LQ109_RS20890 WP_060430992.1 4365500..4366522(-) (pilT) [Serratia marcescens isolate 0]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANVFQQSAGMSLALRRIAGQAPSLAELAVPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDIATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGESAGEGTEG

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=1152830 LQ109_RS20890 WP_060430992.1 4365500..4366522(-) (pilT) [Serratia marcescens isolate 0]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGCCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGTTGCACCGGCCGGGCGGGGAA
CGGCTGCGGGCCAACGTTTTTCAGCAAAGCGCAGGGATGTCTCTCGCGTTGCGGCGCATCGCCGGGCAGGCGCCCTCGCT
TGCCGAGCTGGCGGTGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGACGACGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACATTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGTTCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGTGAGCTGCGCGATATCGCCACCATCCGGC
TGGCGCTCACCGCGGCGGAGACCGGGCATCTGGTGCTGGCGACGTTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGGCGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGCGGTGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCATCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGAGTGCGGGGGAGGGAACGGAGGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.545

97.059

0.529
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.545

97.059

0.529
  pilT Acinetobacter baylyi ADP1

51.36

97.353

0.5
  pilT Acinetobacter baumannii strain A118

50.909

97.059

0.494
  pilT Acinetobacter nosocomialis M2

50.909

97.059

0.494
  pilT Acinetobacter baumannii D1279779

50.909

97.059

0.494
  pilT Pseudomonas aeruginosa PAK

49.555

99.118

0.491
  pilT Neisseria meningitidis 8013

50.301

97.647

0.491
  pilT Neisseria gonorrhoeae MS11

49.702

98.824

0.491
  pilT Pseudomonas stutzeri DSM 10701

48.961

99.118

0.485
  pilT Legionella pneumophila strain ERS1305867

49.398

97.647

0.482
  pilT Legionella pneumophila strain Lp02

49.398

97.647

0.482
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.82

100

0.459
  pilU Vibrio cholerae strain A1552

39.877

95.882

0.382
  pilU Acinetobacter baylyi ADP1

39.938

95

0.379
  pilU Pseudomonas stutzeri DSM 10701

38.889

95.294

0.371

Multiple sequence alignment