Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   LQ078_RS20740 Genome accession   NZ_OW849111
Coordinates   4389945..4390967 (-) Length   340 a.a.
NCBI ID   WP_230116458.1    Uniprot ID   -
Organism   Serratia marcescens isolate 0     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4384945..4395967
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LQ078_RS20710 (AI2616V1_3952) - 4385749..4386261 (+) 513 WP_033654666.1 SprT family zinc-dependent metalloprotease -
  LQ078_RS20715 (AI2616V1_3953) endA 4386363..4387058 (+) 696 WP_033649591.1 deoxyribonuclease I -
  LQ078_RS20720 (AI2616V1_3954) rsmE 4387128..4387859 (+) 732 WP_033654665.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  LQ078_RS20725 (AI2616V1_3955) gshB 4387870..4388820 (+) 951 WP_033654664.1 glutathione synthase -
  LQ078_RS20730 (AI2616V1_3956) - 4388963..4389526 (+) 564 WP_033635940.1 YqgE/AlgH family protein -
  LQ078_RS20735 (AI2616V1_3957) ruvX 4389526..4389948 (+) 423 WP_015379046.1 Holliday junction resolvase RuvX -
  LQ078_RS20740 (AI2616V1_3958) pilT 4389945..4390967 (-) 1023 WP_230116458.1 type IV pilus twitching motility protein PilT Machinery gene
  LQ078_RS20745 (AI2616V1_3959) - 4390988..4391695 (+) 708 WP_033649587.1 YggS family pyridoxal phosphate-dependent enzyme -
  LQ078_RS20750 (AI2616V1_3960) proC 4391715..4392536 (+) 822 WP_033635943.1 pyrroline-5-carboxylate reductase -
  LQ078_RS20755 (AI2616V1_3961) - 4392568..4393122 (+) 555 WP_033635944.1 YggT family protein -
  LQ078_RS20760 (AI2616V1_3962) yggU 4393119..4393409 (+) 291 WP_049235392.1 DUF167 family protein YggU -
  LQ078_RS20765 (AI2616V1_3963) - 4393427..4394020 (+) 594 WP_101455670.1 XTP/dITP diphosphatase -
  LQ078_RS20770 (AI2616V1_3964) hemW 4394013..4395155 (+) 1143 WP_049235393.1 radical SAM family heme chaperone HemW -
  LQ078_RS20775 - 4395192..4395278 (-) 87 Protein_4057 DUF2442 domain-containing protein -

Sequence

Protein


Download         Length: 340 a.a.        Molecular weight: 36476.72 Da        Isoelectric Point: 6.8881

>NTDB_id=1152807 LQ078_RS20740 WP_230116458.1 4389945..4390967(-) (pilT) [Serratia marcescens isolate 0]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELQPLAAAPTLTAQGVQALCDGLLNAQQRESLRRLGQVDLALHRPGGE
RLRANVFQQSAGMSLALRRIAGQAPSLAELAAPAIVPALLRRDDGLLLVTGATGSGKSTTLAAMIDEINRHQPRHILTLE
DPIEFLHRSRRSLIQQREIGRDSHSFDAALRAALREDPDVILLGELRDIATIRLALTAAETGHLVLATLHTRSAPQAVER
LVDVFPTEEKPYVRAQLAGSLQAVIAQKLMRRPGGGRVAIFEVLTATAAVSNLIREGKTHQLASVLQTGAQSGMQTFEQG
LQQRIDAGVLGESTGEGTEG

Nucleotide


Download         Length: 1023 bp        

>NTDB_id=1152807 LQ078_RS20740 WP_230116458.1 4389945..4390967(-) (pilT) [Serratia marcescens isolate 0]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGCCATCCGCCGAT
GTTGCGCATCGACGGTGAACTGCAGCCGCTGGCGGCGGCGCCAACGCTGACGGCGCAAGGCGTGCAGGCCCTCTGCGACG
GATTGCTGAATGCGCAGCAGCGCGAGAGCCTGCGGCGGCTGGGGCAGGTCGATCTGGCGCTGCACCGGCCGGGCGGGGAG
CGGCTGCGGGCCAACGTTTTTCAGCAAAGCGCGGGGATGTCTCTCGCATTGCGGCGCATCGCCGGGCAGGCGCCCTCGCT
TGCCGAGCTGGCGGCGCCGGCCATCGTCCCGGCGCTGCTGCGGCGCGACGACGGGCTTCTCCTGGTCACCGGCGCCACCG
GCAGCGGCAAGTCCACCACGCTGGCGGCGATGATCGACGAGATCAACCGGCACCAGCCGCGGCACATTTTGACGCTGGAG
GATCCGATCGAATTCCTGCACCGCAGCCGGCGTTCGCTGATCCAGCAGCGGGAGATCGGCCGCGACAGCCACAGCTTCGA
TGCGGCGTTGCGCGCCGCGCTGCGCGAAGATCCGGATGTGATCCTGCTGGGTGAGCTGCGCGATATCGCCACCATCCGGC
TGGCGCTCACCGCGGCGGAGACCGGCCATCTGGTGCTGGCGACGTTGCACACCCGCAGCGCGCCGCAGGCGGTGGAACGG
CTGGTGGACGTGTTTCCGACGGAAGAGAAACCCTATGTGCGCGCCCAGCTGGCCGGCAGCCTGCAGGCGGTGATCGCGCA
AAAGCTGATGAGGCGGCCCGGCGGTGGGCGAGTGGCGATCTTTGAGGTGCTGACGGCGACGGCGGCGGTCAGCAACCTGA
TCCGCGAAGGGAAAACCCACCAGTTGGCGAGCGTGTTGCAAACCGGCGCGCAGTCCGGCATGCAAACCTTTGAGCAAGGT
TTGCAGCAGCGGATCGACGCCGGCGTGTTGGGGGAGAGTACGGGGGAGGGAACGGAGGGTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(13-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

54.848

97.059

0.532
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.848

97.059

0.532
  pilT Acinetobacter baylyi ADP1

50.746

98.529

0.5
  pilT Acinetobacter baumannii strain A118

50.299

98.235

0.494
  pilT Acinetobacter nosocomialis M2

50.299

98.235

0.494
  pilT Acinetobacter baumannii D1279779

50.299

98.235

0.494
  pilT Neisseria meningitidis 8013

50.301

97.647

0.491
  pilT Neisseria gonorrhoeae MS11

50

97.647

0.488
  pilT Legionella pneumophila strain ERS1305867

48.955

98.529

0.482
  pilT Legionella pneumophila strain Lp02

48.955

98.529

0.482
  pilT Pseudomonas aeruginosa PAK

49.394

97.059

0.479
  pilT Pseudomonas stutzeri DSM 10701

47.917

98.824

0.474
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.029

100

0.453
  pilU Pseudomonas stutzeri DSM 10701

38.576

99.118

0.382
  pilU Vibrio cholerae strain A1552

39.877

95.882

0.382
  pilU Acinetobacter baylyi ADP1

39.628

95

0.376

Multiple sequence alignment