Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   M2214_RS13725 Genome accession   NZ_OW235315
Coordinates   2799877..2800926 (-) Length   349 a.a.
NCBI ID   WP_248479745.1    Uniprot ID   -
Organism   Tepidibacter aestuarii strain 8C15b     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2794877..2805926
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M2214_RS13700 (T8CH_2814) - 2795405..2795965 (-) 561 WP_248479735.1 PilN domain-containing protein -
  M2214_RS13705 (T8CH_2815) pilM 2795976..2797034 (-) 1059 WP_248479737.1 type IV pilus assembly protein PilM -
  M2214_RS13710 (T8CH_2816) - 2797054..2797803 (-) 750 WP_248479739.1 A24 family peptidase -
  M2214_RS13715 (T8CH_2817) - 2797889..2798245 (-) 357 WP_248479741.1 competence type IV pilus major pilin ComGC -
  M2214_RS13720 (T8CH_2818) - 2798652..2799863 (-) 1212 WP_248479743.1 type II secretion system F family protein -
  M2214_RS13725 (T8CH_2819) pilT 2799877..2800926 (-) 1050 WP_248479745.1 type IV pilus twitching motility protein PilT Machinery gene
  M2214_RS13730 (T8CH_2820) - 2800936..2802615 (-) 1680 WP_248479747.1 GspE/PulE family protein -
  M2214_RS13735 (T8CH_2821) - 2802635..2803318 (-) 684 WP_248479749.1 DUF881 domain-containing protein -
  M2214_RS13740 (T8CH_2822) - 2803601..2805625 (-) 2025 WP_248479751.1 methyl-accepting chemotaxis protein -

Sequence

Protein


Download         Length: 349 a.a.        Molecular weight: 38964.06 Da        Isoelectric Point: 8.6450

>NTDB_id=1152251 M2214_RS13725 WP_248479745.1 2799877..2800926(-) (pilT) [Tepidibacter aestuarii strain 8C15b]
MNIYDLLKKTIELGASDLHITVGYQPVMRINGKLSKYGEDILLPDDNLSLVKQILDEEKFKKLEEKGEMDTSISYHKLGR
FRVNIYKQRGTYCMAIRSVALEIPSMEILGLPPIIKELSKKQRGLVLVTGPTGSGKSTTLASMIDYINKNRSAHILTLED
PIEYLHKHNKSIVNQREIGTDSKSFSNALRSSLRQDPDVILVGEMRDLETISIALTAAETGHLVFSTLHTIGAAKTIDRI
IDAFPSHQQPQILVQLSAVLEGVVSQQLIQKEDGSGRVAALEVMVVNTAIRNLIREGKTHQIQTSIQTGTKFGMQTMDSD
LRNLYLKGIISRESLLTYAVDKDMVSRYI

Nucleotide


Download         Length: 1050 bp        

>NTDB_id=1152251 M2214_RS13725 WP_248479745.1 2799877..2800926(-) (pilT) [Tepidibacter aestuarii strain 8C15b]
ATGAATATATATGATTTACTTAAAAAAACAATAGAATTAGGTGCATCAGATCTTCATATAACAGTTGGATACCAACCTGT
TATGAGAATAAATGGAAAACTGAGTAAGTATGGGGAAGACATATTATTACCAGATGATAATTTGAGTTTAGTAAAGCAAA
TATTAGATGAAGAGAAATTTAAGAAATTAGAAGAAAAAGGAGAAATGGATACATCCATTTCTTATCATAAACTAGGAAGA
TTTAGAGTAAATATATATAAACAAAGAGGAACGTATTGCATGGCTATAAGATCTGTAGCTTTAGAAATACCAAGTATGGA
AATACTAGGTCTTCCACCTATTATAAAAGAATTATCTAAAAAACAAAGAGGTCTAGTTCTTGTAACAGGACCTACAGGAA
GCGGAAAATCGACAACACTCGCATCAATGATTGATTATATAAATAAAAATAGAAGTGCTCACATATTAACATTAGAAGAT
CCTATAGAATATCTTCATAAGCACAATAAGAGTATCGTTAACCAAAGAGAAATAGGTACTGATTCTAAATCATTTTCAAA
TGCTTTAAGATCCTCTTTAAGACAGGATCCGGATGTAATATTAGTTGGAGAGATGAGAGATTTAGAAACAATATCAATAG
CCTTAACAGCAGCAGAAACAGGACATTTAGTATTTTCAACACTTCATACTATAGGTGCTGCTAAAACTATAGATAGAATA
ATAGATGCTTTTCCATCACATCAGCAACCTCAGATATTAGTACAGCTATCTGCAGTTCTTGAAGGTGTTGTATCTCAACA
ATTAATTCAAAAAGAGGATGGAAGTGGAAGGGTTGCTGCCCTTGAGGTAATGGTAGTAAATACAGCAATAAGAAATCTAA
TAAGAGAAGGAAAAACACATCAAATACAAACATCTATACAAACAGGAACAAAATTTGGAATGCAGACTATGGACAGTGAT
CTAAGGAATTTATATTTAAAAGGTATTATATCAAGAGAATCATTGTTAACTTATGCAGTAGACAAGGATATGGTTAGTAG
ATACATATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain ERS1305867

52.035

98.567

0.513
  pilT Legionella pneumophila strain Lp02

52.035

98.567

0.513
  pilT Acinetobacter baumannii strain A118

50.147

97.135

0.487
  pilT Acinetobacter nosocomialis M2

50.147

97.135

0.487
  pilT Acinetobacter baumannii D1279779

50.147

97.135

0.487
  pilT Pseudomonas aeruginosa PAK

50.599

95.702

0.484
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.852

96.562

0.481
  pilT Vibrio cholerae strain A1552

49.852

96.562

0.481
  pilT Acinetobacter baylyi ADP1

50.755

94.842

0.481
  pilT Pseudomonas stutzeri DSM 10701

50

95.702

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.839

99.427

0.476
  pilT Neisseria gonorrhoeae MS11

46.532

99.14

0.461
  pilT Neisseria meningitidis 8013

48.048

95.415

0.458
  pilU Pseudomonas stutzeri DSM 10701

43.363

97.135

0.421
  pilU Acinetobacter baylyi ADP1

42.059

97.421

0.41
  pilU Vibrio cholerae strain A1552

41.538

93.123

0.387

Multiple sequence alignment