Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   KOD61_RS11630 Genome accession   NZ_OU015430
Coordinates   2481306..2482415 (+) Length   369 a.a.
NCBI ID   WP_215218822.1    Uniprot ID   -
Organism   Novilysobacter luteus strain CECT 30171     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2476306..2487415
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KOD61_RS11605 (LYB30171_02317) - 2476792..2477973 (-) 1182 WP_215218817.1 cystathionine gamma-synthase -
  KOD61_RS11610 (LYB30171_02318) - 2478100..2479476 (-) 1377 WP_215218818.1 pyridoxal-phosphate dependent enzyme -
  KOD61_RS11615 (LYB30171_02319) - 2479651..2479869 (-) 219 WP_215218819.1 YdcH family protein -
  KOD61_RS11620 (LYB30171_02320) - 2480244..2480843 (-) 600 WP_215218820.1 hypothetical protein -
  KOD61_RS11625 (LYB30171_02321) - 2480840..2481214 (-) 375 WP_215218821.1 DUF4398 domain-containing protein -
  KOD61_RS11630 (LYB30171_02322) pilU 2481306..2482415 (+) 1110 WP_215218822.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  KOD61_RS11635 (LYB30171_02323) maiA 2482557..2483222 (-) 666 WP_215218823.1 maleylacetoacetate isomerase -
  KOD61_RS11640 (LYB30171_02324) - 2483319..2484305 (-) 987 WP_215218824.1 fumarylacetoacetate hydrolase family protein -
  KOD61_RS11650 (LYB30171_02326) rpsI 2484567..2484959 (-) 393 WP_215218825.1 30S ribosomal protein S9 -
  KOD61_RS11655 (LYB30171_02327) rplM 2484969..2485397 (-) 429 WP_215218826.1 50S ribosomal protein L13 -
  KOD61_RS11660 (LYB30171_02328) - 2485578..2486810 (+) 1233 WP_215218827.1 (Fe-S)-binding protein -

Sequence

Protein


Download         Length: 369 a.a.        Molecular weight: 41152.38 Da        Isoelectric Point: 6.1928

>NTDB_id=1151002 KOD61_RS11630 WP_215218822.1 2481306..2482415(+) (pilU) [Novilysobacter luteus strain CECT 30171]
MDIGYFLKLMTEKNASDMFLSTGAPVYIKIEGRLHPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAYALPEAGR
FRVNVFKQRGEVGMVIRAIRSVIPSIEELQLPQVLKNIIMEPRGLVLIVGSTGSGKSTTLASMIDYRNSNMSGHILTIED
PIEYLHRHKRSLVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDQTTMEAAIAFAETGHLCLATLHSNNADQTLERI
LNFFPEAAHKNVLMNLALNLKAVISQRLVIGTDGRRLPASEVLINTPHIRDLMRRGQVHEIKHAMEESLEEGVETFDQCL
FRLAKEGRIEIEEALRAADSREGLALKFRLSEGAGAEHDPYADVFSPNQ

Nucleotide


Download         Length: 1110 bp        

>NTDB_id=1151002 KOD61_RS11630 WP_215218822.1 2481306..2482415(+) (pilU) [Novilysobacter luteus strain CECT 30171]
ATGGACATCGGCTATTTCCTGAAGCTGATGACGGAGAAGAACGCGTCGGACATGTTCCTGTCCACCGGCGCGCCGGTCTA
CATCAAGATCGAGGGGCGGTTGCATCCGCTCGGCAATACCGGCCTGCCGCCCGGCATGGTCAAGAAGATCGCGTATTCGT
TGATGGACGAGGGCCAAGTTCCGCAGTTCGAGCGCGAACTGGAGCTGAACATGGCCTATGCGCTGCCGGAGGCCGGGCGC
TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAGGTCGGCATGGTGATCCGCGCGATCCGCAGCGTGATCCCGTCGATCGA
GGAGCTGCAGCTGCCGCAGGTGCTCAAGAACATCATCATGGAGCCGCGCGGGCTGGTGCTCATCGTCGGTTCCACCGGCT
CGGGCAAGTCGACCACGCTGGCGTCGATGATCGACTACCGCAACTCGAACATGTCGGGCCACATCCTCACCATCGAGGAC
CCGATCGAATACCTGCACCGGCACAAGCGCTCGCTGGTCAACCAGCGCGAGGTCGGTCTCGACACCCACGCCTTCCACAA
CGCCCTCAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTCGACCAGACCACGATGGAGGCCG
CGATCGCGTTCGCCGAGACGGGCCACCTGTGCCTGGCGACCCTGCACTCCAACAACGCCGATCAGACGCTCGAGCGCATC
CTCAACTTCTTCCCCGAGGCCGCGCACAAGAACGTGCTGATGAACCTCGCGCTTAACCTGAAGGCGGTGATCAGCCAGCG
CTTGGTGATCGGCACCGACGGTCGCCGGCTGCCGGCGTCGGAAGTGCTGATCAACACCCCGCACATCCGCGACCTGATGC
GGCGCGGGCAGGTGCACGAGATCAAGCACGCGATGGAGGAGTCGCTGGAAGAAGGCGTCGAGACCTTCGACCAGTGCCTG
TTCCGGTTGGCGAAGGAAGGCCGGATCGAGATCGAGGAAGCGCTGCGCGCGGCGGACTCGCGCGAGGGCCTGGCGCTGAA
GTTTCGGCTGTCGGAAGGCGCCGGCGCCGAGCACGACCCCTACGCCGACGTGTTTTCGCCAAACCAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.696

97.29

0.493
  pilU Acinetobacter baylyi ADP1

48.739

96.748

0.472
  pilU Vibrio cholerae strain A1552

48.305

95.935

0.463
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.658

98.916

0.442
  pilT Pseudomonas aeruginosa PAK

42.308

91.599

0.388
  pilT Acinetobacter baumannii strain A118

41.57

93.225

0.388
  pilT Acinetobacter nosocomialis M2

41.57

93.225

0.388
  pilT Acinetobacter baumannii D1279779

41.57

93.225

0.388
  pilT Acinetobacter baylyi ADP1

41.279

93.225

0.385
  pilT Pseudomonas stutzeri DSM 10701

40.988

93.225

0.382
  pilT Neisseria gonorrhoeae MS11

40.058

94.038

0.377
  pilT Neisseria meningitidis 8013

40.058

94.038

0.377
  pilT Legionella pneumophila strain ERS1305867

40.533

91.599

0.371
  pilT Legionella pneumophila strain Lp02

40.533

91.599

0.371
  pilT Vibrio cholerae strain A1552

41.141

90.244

0.371
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.141

90.244

0.371

Multiple sequence alignment