Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DG357_RS18945 Genome accession   NZ_LT992502
Coordinates   3924574..3925554 (-) Length   326 a.a.
NCBI ID   WP_088204256.1    Uniprot ID   -
Organism   Enterobacter bugandensis isolate EB-247     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3919574..3930554
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DG357_RS18915 - 3920464..3920961 (+) 498 WP_088204254.1 SprT family zinc-dependent metalloprotease -
  DG357_RS18920 endA 3921056..3921763 (+) 708 WP_028014365.1 deoxyribonuclease I -
  DG357_RS18925 rsmE 3921815..3922546 (+) 732 WP_041908327.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  DG357_RS18930 gshB 3922566..3923513 (+) 948 WP_041908326.1 glutathione synthase -
  DG357_RS18935 - 3923588..3924148 (+) 561 WP_028014368.1 YqgE/AlgH family protein -
  DG357_RS18940 ruvX 3924148..3924564 (+) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  DG357_RS18945 pilT 3924574..3925554 (-) 981 WP_088204256.1 type IV pilus twitching motility protein PilT Machinery gene
  DG357_RS18950 - 3925572..3926273 (+) 702 WP_047367421.1 YggS family pyridoxal phosphate-dependent enzyme -
  DG357_RS18955 - 3926295..3926861 (+) 567 WP_045260768.1 YggT family protein -
  DG357_RS18960 yggU 3926858..3927154 (+) 297 WP_047364545.1 DUF167 family protein YggU -
  DG357_RS18965 - 3927158..3927751 (+) 594 WP_010435473.1 XTP/dITP diphosphatase -
  DG357_RS18970 hemW 3927744..3928886 (+) 1143 WP_088204257.1 radical SAM family heme chaperone HemW -
  DG357_RS18975 - 3929091..3929807 (-) 717 WP_041908321.1 DUF2884 domain-containing protein -
  DG357_RS18980 - 3929865..3930191 (-) 327 WP_028014375.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35627.71 Da        Isoelectric Point: 6.3904

>NTDB_id=1150364 DG357_RS18945 WP_088204256.1 3924574..3925554(-) (pilT) [Enterobacter bugandensis isolate EB-247]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGAWWANGQVDFAVTVTGDQRLRG
SAFKQMKGISVTLRLLPRSCPQLPSLGVPRAIPELLSSDNGLILVTGATGSGKSTTLAAMIDFLNRHTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1150364 DG357_RS18945 WP_088204256.1 3924574..3925554(-) (pilT) [Enterobacter bugandensis isolate EB-247]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGATTGGGCCGCCTTGAGCCGGCCCCGTTTCCGCCGCCGGATGTCGGGGCGTTGCTAAAGGCGTGGCTCAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTCGATTTTGCCGTGACGGTCACAGGAGACCAGCGTCTGCGCGGC
AGTGCGTTTAAGCAGATGAAGGGCATCTCCGTTACCCTGCGGCTGTTGCCGCGTTCCTGTCCGCAGCTCCCTTCGCTTGG
GGTGCCGCGCGCTATTCCCGAACTGTTGTCCAGCGACAACGGGTTGATTCTGGTAACGGGAGCGACCGGCAGCGGTAAAT
CCACCACGCTGGCCGCAATGATCGACTTCCTCAACCGCCATACTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTGATTCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCAGAGGCGCTGCG
CAGTGCGTTACGCGAGGACCCGGACGTTATTTTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAGACCGGGCATCTGGTGCTGGCGACGCTGCACACGCGCGGGGCAGCACAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCTGGCAGCCTGCGGGCAGTGCTGGCGCAAAAGCTGCTTCC
CGATCTTCAGGGCGGGCGCGTCGCGCTGTACGAGCTGCTGGTAAACACCGCGGCGGCGGCGAATCTGATTCGTGAGGGGA
AGACGTGGCAGCTGCCCGGCATCATTCAAACCGGCCAGCAGGCGGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCACAGGGGCGGCTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Pseudomonas stutzeri DSM 10701

48.93

100

0.491
  pilT Legionella pneumophila strain Lp02

49.689

98.773

0.491
  pilT Legionella pneumophila strain ERS1305867

49.689

98.773

0.491
  pilT Neisseria meningitidis 8013

48.476

100

0.488
  pilT Neisseria gonorrhoeae MS11

48.171

100

0.485
  pilT Acinetobacter baumannii strain A118

48.318

100

0.485
  pilT Acinetobacter baumannii D1279779

48.318

100

0.485
  pilT Acinetobacter baylyi ADP1

48.318

100

0.485
  pilT Pseudomonas aeruginosa PAK

48.012

100

0.482
  pilT Acinetobacter nosocomialis M2

48.75

98.16

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.168

98.773

0.426
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment