Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   C6H34_RS24855 Genome accession   NZ_LT963395
Coordinates   5345251..5346285 (-) Length   344 a.a.
NCBI ID   WP_065350798.1    Uniprot ID   A0A193SVW9
Organism   Pseudomonas cerasi isolate PL963     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5340251..5351285
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C6H34_RS24830 (PL963_04856) pyrR 5340934..5341446 (+) 513 WP_003366144.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  C6H34_RS24835 (PL963_04857) - 5341459..5342463 (+) 1005 WP_003366142.1 aspartate carbamoyltransferase catalytic subunit -
  C6H34_RS24840 (PL963_04858) - 5342460..5343731 (+) 1272 WP_003366140.1 dihydroorotase -
  C6H34_RS24845 (PL963_04859) - 5343823..5344227 (-) 405 WP_065350797.1 NINE protein -
  C6H34_RS24850 (PL963_04860) - 5344478..5345155 (+) 678 WP_003432065.1 C40 family peptidase -
  C6H34_RS24855 (PL963_04861) pilT 5345251..5346285 (-) 1035 WP_065350798.1 type IV pilus twitching motility protein PilT Machinery gene
  C6H34_RS24860 (PL963_04862) - 5346341..5347027 (+) 687 WP_003366136.1 YggS family pyridoxal phosphate-dependent enzyme -
  C6H34_RS24865 (PL963_04863) proC 5347049..5347867 (+) 819 WP_065350799.1 pyrroline-5-carboxylate reductase -
  C6H34_RS24870 (PL963_04864) - 5347893..5348483 (+) 591 WP_065350800.1 YggT family protein -
  C6H34_RS24880 (PL963_04865) metX 5348781..5349920 (+) 1140 WP_003432070.1 homoserine O-succinyltransferase MetX -
  C6H34_RS24885 (PL963_04866) metW 5349928..5350548 (+) 621 WP_003316724.1 methionine biosynthesis protein MetW -
  C6H34_RS24890 (PL963_04867) rdgB 5350582..5351175 (+) 594 WP_003366128.1 RdgB/HAM1 family non-canonical purine NTP pyrophosphatase -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37939.72 Da        Isoelectric Point: 7.0295

>NTDB_id=1148798 C6H34_RS24855 WP_065350798.1 5345251..5346285(-) (pilT) [Pseudomonas cerasi isolate PL963]
MDITELLAFSAKQGASDLHLSAGLPPMIRVDGDVRRINLPPLDAKEVKALIYDIMNDKQRQDFEERLETDFSFEVSGVAR
FRVNAFNQNRGAGAVFRTIPSKILSMEDLGMGSVFRKITDVARGLILVTGPTGSGKSTTLAAMIDYLNNNKHHHILTIED
PIEFVHESKKCLVNQREVHRDTLGFSEALRSALREDPDVILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRI
VDVFPAQEKSMIRSMLSESLHAVVSQALLKKVGGGRVAAHEIMMGTPAIRNLIREDKVAQMYSSIQTGGSMGMQTLDMCL
ADLVKKGLITRESARERAKVPDNF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1148798 C6H34_RS24855 WP_065350798.1 5345251..5346285(-) (pilT) [Pseudomonas cerasi isolate PL963]
ATGGATATTACCGAGCTGCTGGCCTTCAGTGCCAAACAGGGCGCGTCGGATTTGCACCTCTCTGCAGGTCTGCCGCCGAT
GATCCGCGTCGACGGCGATGTACGGCGTATCAACCTGCCTCCGCTGGACGCGAAGGAGGTCAAGGCGCTGATCTACGACA
TCATGAACGACAAGCAACGGCAGGACTTCGAAGAACGGCTGGAAACCGACTTTTCCTTCGAAGTGTCGGGCGTGGCGCGG
TTTCGGGTCAACGCTTTCAACCAGAACCGCGGTGCTGGCGCGGTCTTCCGGACCATCCCTTCGAAAATCCTGAGCATGGA
AGACTTGGGCATGGGCAGTGTGTTTCGGAAGATTACCGACGTGGCGCGTGGCCTGATTCTGGTCACCGGTCCGACCGGCT
CAGGCAAGTCGACCACCCTGGCGGCGATGATCGACTACCTGAATAACAACAAGCATCACCACATTCTGACCATCGAGGAC
CCGATCGAATTCGTCCACGAGTCGAAGAAGTGCCTGGTCAACCAGCGCGAGGTGCACCGCGACACACTGGGCTTCTCGGA
GGCGCTGCGCTCGGCGTTGCGTGAAGACCCGGACGTGATTCTGGTCGGGGAGATGCGTGACCTGGAGACCATACGCCTGG
CGCTGACCGCAGCTGAAACCGGCCACCTGGTATTCGGCACGCTGCACACCACATCAGCAGCCAAGACCATCGACCGGATC
GTCGATGTGTTTCCGGCTCAGGAAAAATCGATGATTCGCTCGATGCTCTCCGAATCGCTGCACGCCGTGGTTTCGCAGGC
GTTGCTCAAGAAGGTCGGCGGCGGACGCGTGGCGGCGCACGAAATCATGATGGGCACCCCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGTCGATCCAGACGGGCGGCTCGATGGGCATGCAGACGCTGGACATGTGCCTG
GCCGATCTGGTGAAGAAGGGGCTGATCACCCGCGAAAGCGCGCGCGAACGTGCCAAGGTGCCGGATAACTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A193SVW9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas stutzeri DSM 10701

89.244

100

0.892
  pilT Pseudomonas aeruginosa PAK

87.791

100

0.878
  pilT Acinetobacter nosocomialis M2

78.488

100

0.785
  pilT Acinetobacter baumannii D1279779

78.488

100

0.785
  pilT Acinetobacter baumannii strain A118

78.488

100

0.785
  pilT Acinetobacter baylyi ADP1

78.198

100

0.782
  pilT Legionella pneumophila strain Lp02

74.26

98.256

0.73
  pilT Legionella pneumophila strain ERS1305867

74.26

98.256

0.73
  pilT Neisseria meningitidis 8013

67.941

98.837

0.672
  pilT Neisseria gonorrhoeae MS11

67.647

98.837

0.669
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.469

97.965

0.651
  pilT Vibrio cholerae strain A1552

66.469

97.965

0.651
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.333

100

0.535
  pilU Acinetobacter baylyi ADP1

41.04

100

0.413
  pilU Pseudomonas stutzeri DSM 10701

42.09

97.384

0.41
  pilU Vibrio cholerae strain A1552

40.299

97.384

0.392

Multiple sequence alignment