Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   CKW06_RS02825 Genome accession   NZ_LT906480
Coordinates   591564..592691 (-) Length   375 a.a.
NCBI ID   WP_004141526.1    Uniprot ID   A0A854E1G1
Organism   Stenotrophomonas maltophilia strain NCTC10257     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 586564..597691
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKW06_RS02805 (SAMEA4076705_00569) - 586991..587980 (+) 990 WP_024957361.1 fumarylacetoacetate hydrolase family protein -
  CKW06_RS02810 (SAMEA4076705_00570) maiA 587995..588663 (+) 669 WP_024957360.1 maleylacetoacetate isomerase -
  CKW06_RS02815 (SAMEA4076705_00571) - 588709..590169 (-) 1461 WP_024957359.1 UdgX family uracil-DNA binding protein -
  CKW06_RS02820 (SAMEA4076705_00572) - 590150..591433 (-) 1284 WP_024957358.1 putative DNA modification/repair radical SAM protein -
  CKW06_RS02825 (SAMEA4076705_00573) pilU 591564..592691 (-) 1128 WP_004141526.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  CKW06_RS02830 (SAMEA4076705_00574) - 592813..593178 (+) 366 WP_024957357.1 DUF4398 domain-containing protein -
  CKW06_RS02835 (SAMEA4076705_00575) - 593175..594059 (+) 885 WP_005407962.1 hypothetical protein -
  CKW06_RS02840 (SAMEA4076705_00577) - 594384..594620 (+) 237 WP_005407964.1 YdcH family protein -
  CKW06_RS02845 (SAMEA4076705_00578) - 594921..596291 (+) 1371 WP_024957356.1 pyridoxal-phosphate dependent enzyme -
  CKW06_RS02850 (SAMEA4076705_00579) - 596415..597599 (+) 1185 WP_024957355.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41398.70 Da        Isoelectric Point: 6.3741

>NTDB_id=1148067 CKW06_RS02825 WP_004141526.1 591564..592691(-) (pilU) [Stenotrophomonas maltophilia strain NCTC10257]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLLNTPMIRDLLRRGQVHEIKAAMEASLEEGMESFDQCL
FRLAKAGTIEQEEALRAADSRDGLALKFRLSEGSSGEHDPYADFSAGAPSITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=1148067 CKW06_RS02825 WP_004141526.1 591564..592691(-) (pilU) [Stenotrophomonas maltophilia strain NCTC10257]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTTCTGACCACCGGGGCACCGGTCTA
CATCAAGATCGAGGGGAAGCTCTATCCGCTGGGCAACACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAGCTCAACATGGCCATCGCCCTGCCCGATGCCGGTCGC
TTCCGCGTCAATGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTCATCCGTGCCATCCGCAGCCGGATTCCGAGCATCGA
GGAGCTGAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACCCCGCGCGGGCTGGTACTGGTGGTGGGGTCCACCGGTT
CCGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAAGAC
CCGATCGAATACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGGCTGGATACCCATGCCTTCCACAA
CGCGCTGAAGAACGCCATGCGTGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCAGAGACGATGGAAGCGG
CGATCGCCTTCGCCGAAACCGGCCACCTGTGCTTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGTGCGGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGAAGGCCGTCGCCTGCCGGCCACCGAAGTGCTGCTCAACACGCCGATGATCCGCGACCTGCTGC
GTCGCGGCCAGGTGCACGAGATCAAGGCGGCGATGGAAGCATCGTTGGAAGAGGGCATGGAGAGTTTCGACCAGTGCCTG
TTCCGGCTGGCCAAGGCCGGCACCATCGAGCAGGAGGAGGCGCTGCGCGCGGCCGACTCGCGCGATGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCAGCAGCGGCGAGCATGACCCGTACGCGGATTTCTCGGCCGGCGCACCCAGCATCACCCACG
GCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A854E1G1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.975

95.733

0.488
  pilU Vibrio cholerae strain A1552

49.435

94.4

0.467
  pilU Acinetobacter baylyi ADP1

49.153

94.4

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.04

99.467

0.448
  pilT Pseudomonas aeruginosa PAK

42.604

90.133

0.384
  pilT Acinetobacter baumannii strain A118

41.42

90.133

0.373
  pilT Acinetobacter nosocomialis M2

41.42

90.133

0.373
  pilT Acinetobacter baumannii D1279779

41.42

90.133

0.373
  pilT Acinetobacter baylyi ADP1

41.176

90.667

0.373
  pilT Pseudomonas stutzeri DSM 10701

41.124

90.133

0.371
  pilT Legionella pneumophila strain Lp02

40.533

90.133

0.365
  pilT Legionella pneumophila strain ERS1305867

40.533

90.133

0.365
  pilT Vibrio cholerae strain A1552

41.141

88.8

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.141

88.8

0.365
  pilT Neisseria gonorrhoeae MS11

38.905

92.533

0.36
  pilT Neisseria meningitidis 8013

38.905

92.533

0.36

Multiple sequence alignment