Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CKW09_RS20710 Genome accession   NZ_LT906479
Coordinates   4400934..4401989 (-) Length   351 a.a.
NCBI ID   WP_095099182.1    Uniprot ID   A0A240CBR4
Organism   Serratia ficaria strain NCTC12148     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4395934..4406989
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKW09_RS20680 (SAMEA4384070_04140) - 4396755..4397267 (+) 513 WP_061798585.1 SprT family zinc-dependent metalloprotease -
  CKW09_RS20685 (SAMEA4384070_04141) endA 4397369..4398064 (+) 696 WP_061798583.1 deoxyribonuclease I -
  CKW09_RS20690 (SAMEA4384070_04142) rsmE 4398138..4398869 (+) 732 WP_061798581.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  CKW09_RS20695 (SAMEA4384070_04143) gshB 4398880..4399830 (+) 951 WP_061798580.1 glutathione synthase -
  CKW09_RS20700 (SAMEA4384070_04144) - 4399986..4400549 (+) 564 WP_061798700.1 YqgE/AlgH family protein -
  CKW09_RS20705 (SAMEA4384070_04145) ruvX 4400549..4400971 (+) 423 WP_061798578.1 Holliday junction resolvase RuvX -
  CKW09_RS20710 (SAMEA4384070_04146) pilT 4400934..4401989 (-) 1056 WP_095099182.1 type IV pilus twitching motility protein PilT Machinery gene
  CKW09_RS20715 (SAMEA4384070_04147) - 4402010..4402717 (+) 708 WP_095099185.1 YggS family pyridoxal phosphate-dependent enzyme -
  CKW09_RS20720 (SAMEA4384070_04148) proC 4402736..4403557 (+) 822 WP_061798574.1 pyrroline-5-carboxylate reductase -
  CKW09_RS20725 (SAMEA4384070_04149) - 4403589..4404143 (+) 555 WP_033635944.1 YggT family protein -
  CKW09_RS20730 (SAMEA4384070_04150) yggU 4404140..4404430 (+) 291 WP_061798572.1 DUF167 family protein YggU -
  CKW09_RS20735 (SAMEA4384070_04151) - 4404448..4405041 (+) 594 WP_061798571.1 XTP/dITP diphosphatase -
  CKW09_RS20740 (SAMEA4384070_04152) hemW 4405034..4406173 (+) 1140 WP_061798569.1 radical SAM family heme chaperone HemW -
  CKW09_RS20745 (SAMEA4384070_04153) - 4406214..4406462 (-) 249 WP_061798567.1 DUF2442 domain-containing protein -
  CKW09_RS20750 (SAMEA4384070_04154) - 4406446..4406688 (-) 243 WP_061798564.1 DUF4160 domain-containing protein -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 38404.06 Da        Isoelectric Point: 7.0686

>NTDB_id=1148059 CKW09_RS20710 WP_095099182.1 4400934..4401989(-) (pilT) [Serratia ficaria strain NCTC12148]
MDIDEFVALSVKHNASDLHLCAGHPPMLRIDGELRPLEGAESLRPERMLALCDRLLAPSQRRELQQRGQLDLALQLPGGL
RLRANAFLQREGISLALRRIASECPTLAALAAPEIIPALLRREDGLILVTGATGSGKSTTLAAMIDEINRHQRRHILTLE
DPIEFVHHSRRSLIQQRELGRDSHSFDDALRAALREDPDVILLGELRDTATIRLALTAAETGHLVLATLHTRNAPQAIER
LVDVFPAEEKPYVRAQLAGSLQAVIAQKLIGRPGGGRLAIFEVLTATAAVSSMIREGKNHQLASVLQTGAQSGMQTFEQG
LQQRIDAGLLGDRGERERAVSPVAFRTSFPE

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=1148059 CKW09_RS20710 WP_095099182.1 4400934..4401989(-) (pilT) [Serratia ficaria strain NCTC12148]
ATGGATATCGATGAATTCGTGGCCCTTAGTGTAAAGCATAATGCTTCCGATCTGCACCTTTGTGCCGGTCATCCGCCGAT
GTTGCGCATCGACGGTGAATTGCGGCCGCTCGAAGGGGCGGAAAGCCTGAGGCCGGAGCGCATGCTGGCCCTGTGCGACC
GGCTGCTGGCGCCTTCGCAGCGCCGCGAACTGCAGCAGCGCGGGCAGCTCGATCTGGCGCTGCAATTGCCGGGCGGTTTG
CGGCTGCGCGCCAACGCGTTTTTACAACGCGAGGGTATTTCCCTGGCGCTGCGAAGGATAGCCAGCGAGTGCCCGACGCT
GGCGGCCTTGGCGGCGCCGGAGATTATCCCGGCGCTGCTGCGGCGCGAAGACGGCCTGATATTGGTCACCGGCGCCACCG
GCAGCGGCAAATCCACCACGCTGGCGGCGATGATCGACGAGATTAACCGCCACCAGCGGCGGCATATTCTCACGTTGGAG
GACCCCATCGAGTTTGTGCATCACAGCCGACGTTCGCTGATCCAGCAGCGTGAGCTCGGCCGCGACAGCCACAGCTTTGA
TGATGCGTTGCGCGCCGCGCTGCGGGAGGATCCGGACGTGATCCTGCTGGGGGAACTGCGCGACACCGCCACCATTCGCC
TGGCGCTCACCGCGGCGGAAACCGGGCATCTGGTGCTGGCGACGCTGCATACCCGCAACGCGCCGCAGGCGATTGAGCGG
CTGGTGGACGTCTTTCCGGCGGAGGAGAAGCCCTACGTACGCGCCCAACTGGCCGGCAGTCTGCAGGCGGTGATCGCACA
GAAGCTGATCGGGCGCCCCGGCGGCGGCCGGCTGGCGATTTTTGAAGTGCTGACGGCGACGGCGGCGGTCAGCAGCATGA
TCCGCGAAGGCAAAAACCACCAGCTTGCCAGCGTGCTGCAAACCGGCGCGCAGTCCGGCATGCAGACCTTTGAACAGGGC
CTGCAGCAGCGGATCGACGCCGGTTTGCTGGGGGACAGGGGAGAACGAGAACGGGCGGTTAGCCCAGTTGCTTTTCGAAC
CAGCTTTCCAGAATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A240CBR4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

54.848

94.017

0.516
  pilT Vibrio cholerae strain A1552

54.848

94.017

0.516
  pilT Acinetobacter nosocomialis M2

49.565

98.291

0.487
  pilT Acinetobacter baumannii D1279779

49.565

98.291

0.487
  pilT Acinetobacter baumannii strain A118

49.565

98.291

0.487
  pilT Acinetobacter baylyi ADP1

49.565

98.291

0.487
  pilT Neisseria meningitidis 8013

50

96.866

0.484
  pilT Pseudomonas aeruginosa PAK

49.275

98.291

0.484
  pilT Neisseria gonorrhoeae MS11

49.706

96.866

0.481
  pilT Pseudomonas stutzeri DSM 10701

48.406

98.291

0.476
  pilT Legionella pneumophila strain ERS1305867

47.536

98.291

0.467
  pilT Legionella pneumophila strain Lp02

47.536

98.291

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.665

98.575

0.45
  pilU Acinetobacter baylyi ADP1

39.08

99.145

0.387
  pilU Vibrio cholerae strain A1552

41.411

92.877

0.385
  pilU Pseudomonas stutzeri DSM 10701

39.815

92.308

0.368

Multiple sequence alignment