Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CKV72_RS04265 Genome accession   NZ_LT906477
Coordinates   875393..876448 (+) Length   351 a.a.
NCBI ID   WP_089862900.1    Uniprot ID   -
Organism   Clostridium cochlearium strain NCTC13027     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 870393..881448
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CKV72_RS04245 (SAMEA4530647_00889) udk 871411..872043 (+) 633 WP_089862904.1 uridine kinase -
  CKV72_RS04250 (SAMEA4530647_00890) - 872209..873741 (+) 1533 WP_143012616.1 penicillin-binding transpeptidase domain-containing protein -
  CKV72_RS04255 (SAMEA4530647_00891) sigK 873843..874532 (+) 690 WP_171303677.1 RNA polymerase sporulation sigma factor SigK -
  CKV72_RS04260 (SAMEA4530647_00892) aroE 874606..875409 (+) 804 WP_089862901.1 shikimate dehydrogenase -
  CKV72_RS04265 (SAMEA4530647_00893) pilT 875393..876448 (+) 1056 WP_089862900.1 type IV pilus twitching motility protein PilT Machinery gene
  CKV72_RS04270 (SAMEA4530647_00894) - 876670..877950 (+) 1281 WP_095177573.1 glycosyltransferase family 2 protein -
  CKV72_RS04275 (SAMEA4530647_00895) ftsA 878070..879332 (+) 1263 WP_095177574.1 cell division protein FtsA -
  CKV72_RS04280 (SAMEA4530647_00896) ftsZ 879348..880457 (+) 1110 WP_089862897.1 cell division protein FtsZ -
  CKV72_RS04285 (SAMEA4530647_00897) spoIIGA 880608..881411 (+) 804 WP_089862896.1 sigma-E processing peptidase SpoIIGA -

Sequence

Protein


Download         Length: 351 a.a.        Molecular weight: 39105.37 Da        Isoelectric Point: 8.0828

>NTDB_id=1147992 CKV72_RS04265 WP_089862900.1 875393..876448(+) (pilT) [Clostridium cochlearium strain NCTC13027]
MESLSDLLDKTLQKKGSDLHLTVGVAPTIRVNGELTQISDEKLKPKELEKYAQEILKEDFQEYCEIGEADTSFSIPGLGR
FRVNVFKQRGSHAIAIRIVGVRVPSLNTLDFPKTIKELSLMKRGLILVTGPAGSGKSTTLAAMINEINLKRSAHIITLED
PIEYLHKHNNSIVNQREIGKDTKNYTQALRAVLREDPDVILVGEMRDLETISIALTAAETGHLVLSSLHTIGAAKTIDRI
IDVFPSSQQQQIRIQLATVLKGIVSQQLVPRKDGDGRIAALEIMIANNAIQNMIREGKVHQIESSIQTGIKYGMKTMDMA
LAELYKKELISYDNAIAYSMDREMIKKMIYI

Nucleotide


Download         Length: 1056 bp        

>NTDB_id=1147992 CKV72_RS04265 WP_089862900.1 875393..876448(+) (pilT) [Clostridium cochlearium strain NCTC13027]
TTGGAAAGTTTAAGTGATTTATTAGATAAAACATTGCAAAAAAAGGGTTCAGATTTACACCTTACAGTAGGGGTAGCTCC
AACAATTAGGGTAAATGGAGAATTAACTCAAATATCTGATGAAAAATTGAAACCTAAAGAATTGGAAAAATATGCACAAG
AAATTCTCAAGGAAGATTTTCAGGAGTATTGTGAAATAGGGGAGGCAGACACATCTTTTTCCATACCTGGATTAGGAAGA
TTTAGAGTCAATGTATTCAAACAAAGAGGAAGCCATGCTATTGCCATAAGAATTGTAGGTGTAAGAGTGCCAAGTTTAAA
TACCTTAGATTTTCCTAAGACTATAAAAGAATTGTCTCTTATGAAAAGAGGGCTTATATTAGTGACAGGGCCTGCAGGAT
CTGGTAAGAGTACTACTTTAGCAGCAATGATAAATGAAATCAATTTAAAGAGGTCTGCTCATATAATAACATTAGAAGAT
CCAATAGAATATCTTCATAAACATAATAATTCTATTGTGAATCAAAGAGAAATAGGCAAAGATACAAAAAATTATACTCA
AGCTCTTAGGGCGGTTTTAAGAGAAGATCCAGATGTTATATTAGTTGGGGAGATGAGAGATCTAGAAACTATTTCAATAG
CTTTAACAGCTGCAGAAACAGGACATTTAGTATTATCTAGTTTACATACTATTGGAGCAGCTAAAACTATAGATAGAATA
ATAGATGTTTTCCCTTCATCACAACAACAGCAAATAAGAATACAGTTAGCTACTGTTTTAAAGGGAATAGTATCTCAACA
ATTGGTTCCTAGAAAAGACGGAGATGGCAGAATTGCAGCTTTAGAAATCATGATTGCAAATAATGCAATACAAAATATGA
TAAGAGAAGGTAAAGTTCATCAAATAGAATCTTCCATTCAAACTGGAATTAAATATGGTATGAAAACTATGGACATGGCT
TTAGCAGAATTATATAAAAAAGAATTAATTTCTTATGACAATGCAATAGCATATTCTATGGATAGAGAAATGATTAAAAA
AATGATATATATATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(111-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baylyi ADP1

49.708

97.436

0.484
  pilT Pseudomonas aeruginosa PAK

49.258

96.011

0.473
  pilT Legionella pneumophila strain ERS1305867

48.105

97.721

0.47
  pilT Legionella pneumophila strain Lp02

48.105

97.721

0.47
  pilT Pseudomonas stutzeri DSM 10701

48.368

96.011

0.464
  pilT Acinetobacter nosocomialis M2

48.204

95.157

0.459
  pilT Acinetobacter baumannii D1279779

48.204

95.157

0.459
  pilT Acinetobacter baumannii strain A118

48.204

95.157

0.459
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.532

98.575

0.459
  pilT Neisseria meningitidis 8013

47.748

94.872

0.453
  pilT Vibrio cholerae strain A1552

48.024

93.732

0.45
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.024

93.732

0.45
  pilT Neisseria gonorrhoeae MS11

47.447

94.872

0.45
  pilU Pseudomonas stutzeri DSM 10701

40.988

98.006

0.402
  pilU Acinetobacter baylyi ADP1

40.588

96.866

0.393
  pilU Vibrio cholerae strain A1552

39.222

95.157

0.373

Multiple sequence alignment