Detailed information NTG1147448

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: CKV84_RS12125
• Genome accession: NZ_LT906452
• Coordinates: 2664398..2665519 (-)
• Length: 373 a.a.
• NCBI ID: WP_014842283.1
• Uniprot ID: A0AAV2V075
• Organism: Legionella pneumophila strain NCTC11985
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2659398..2670519

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CKV84_RS12110 (SAMEA4040028_02405)	-	2660284..2660721 (-)	438	WP_015444141.1	hypothetical protein	-
CKV84_RS12115 (SAMEA4040028_02406)	-	2660998..2662293 (+)	1296	WP_014842281.1	serine/threonine transporter	-
CKV84_RS12120 (SAMEA4040028_02407)	ankK	2662386..2664311 (-)	1926	WP_014842282.1	Dot/Icm T4SS effector AnkK/LegA5	-
CKV84_RS12125 (SAMEA4040028_02408)	pilU	2664398..2665519 (-)	1122	WP_014842283.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
CKV84_RS12130 (SAMEA4040028_02409)	-	2665609..2667537 (-)	1929	WP_014842284.1	FAD/FMN-containing dehydrogenase	-
CKV84_RS12135 (SAMEA4040028_02410)	-	2667562..2668392 (-)	831	WP_027220503.1	phytanoyl-CoA dioxygenase family protein	-
CKV84_RS12140 (SAMEA4040028_02411)	radA	2668389..2669744 (-)	1356	WP_027220504.1	DNA repair protein RadA	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 41732.03 Da
• Isoelectric Point: 5.4724

>NTDB_id=1147448 CKV84_RS12125 WP_014842283.1 2664398..2665519(-) (pilU) [Legionella pneumophila strain NCTC11985]
MDITPFFKLMVDKGASDLFFSVGAPPNIKIEGITSPIGQVPIKSNQMAEIVLSIMNDAQQKEFESKMELNMAFSLAGVGR
FRINLFRQRGEVAMVVRYIKGEIPSIEDLNLPQILKSLVLELRGLVLVVGSTGSGKSTTLATMINHRNENHRGHILTIED
PIEYIHQHKKSIVDQREIGIDTLSYDNALKNAMREAPDVILIGEIRDLNSMKHAIAYAETGHLCISTLHANNANQALDRV
INFFPDEARQQILLDLSLNLKAIISLRLIPGVDNQRVAAVEILINSPYISDLIEKGKIDDIKEVMARSTEQGMQTFDQAL
FNLYKEGKITKENAIKYADSKNNVGLQIRLGEEKSFNIQDENLQIQPDDIDKV

Nucleotide

• Length: 1122 bp

>NTDB_id=1147448 CKV84_RS12125 WP_014842283.1 2664398..2665519(-) (pilU) [Legionella pneumophila strain NCTC11985]
ATGGATATTACTCCTTTTTTTAAGCTCATGGTCGATAAAGGTGCCTCAGATTTGTTTTTCAGTGTCGGGGCACCACCAAA
TATTAAAATCGAGGGCATCACATCACCTATTGGCCAGGTTCCTATCAAATCGAATCAAATGGCAGAAATTGTTTTATCTA
TTATGAATGACGCACAGCAAAAAGAATTTGAGTCTAAAATGGAACTCAATATGGCTTTTTCATTAGCTGGAGTAGGGCGT
TTCCGAATCAATTTATTCAGGCAACGTGGCGAAGTGGCTATGGTGGTGCGTTACATCAAGGGCGAAATACCTTCTATAGA
AGATTTAAATCTTCCTCAAATTTTAAAGTCTCTGGTCTTGGAGTTGCGAGGTTTAGTATTGGTTGTTGGGTCCACGGGAT
CTGGTAAATCAACAACTCTGGCCACGATGATCAACCACCGTAATGAAAATCACAGAGGGCATATTTTAACAATAGAGGAT
CCTATTGAATATATACATCAACATAAAAAATCAATAGTTGATCAACGAGAGATAGGTATTGATACTCTAAGCTATGACAA
TGCATTGAAAAACGCAATGAGAGAAGCGCCTGATGTGATATTAATTGGTGAAATTCGTGATCTTAACTCAATGAAACATG
CGATAGCTTATGCTGAAACAGGACATTTGTGCATTTCTACTTTGCATGCCAATAACGCCAATCAGGCTCTGGATAGAGTG
ATTAATTTTTTCCCCGATGAGGCAAGACAACAGATATTATTAGATTTATCTCTTAATTTAAAAGCAATCATTTCATTACG
TTTGATACCAGGAGTTGATAATCAACGTGTCGCAGCGGTGGAGATCTTGATTAACTCTCCTTACATTTCCGATTTGATTG
AAAAAGGGAAGATAGACGATATTAAAGAAGTCATGGCAAGAAGCACGGAACAAGGCATGCAGACTTTCGATCAGGCTTTA
TTTAATTTATATAAAGAAGGAAAAATTACCAAAGAAAATGCGATTAAGTATGCTGATTCAAAAAACAATGTGGGTTTACA
AATTCGATTGGGCGAAGAAAAAAGCTTTAATATTCAAGATGAGAATTTACAAATACAACCCGATGATATTGACAAGGTAT
GA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (43-268)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.085	94.906	0.523
pilU	Acinetobacter baylyi ADP1	52.958	95.174	0.504
pilU	Vibrio cholerae strain A1552	48	93.834	0.45
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	45.428	90.885	0.413
pilT	Legionella pneumophila strain ERS1305867	42.982	91.689	0.394
pilT	Legionella pneumophila strain Lp02	42.982	91.689	0.394
pilT	Neisseria meningitidis 8013	42.075	93.029	0.391
pilT	Neisseria gonorrhoeae MS11	42.075	93.029	0.391
pilT	Pseudomonas aeruginosa PAK	42.274	91.957	0.389
pilT	Pseudomonas stutzeri DSM 10701	41.691	91.957	0.383
pilT	Acinetobacter baumannii strain A118	42.814	89.544	0.383
pilT	Acinetobacter nosocomialis M2	42.814	89.544	0.383
pilT	Acinetobacter baumannii D1279779	42.814	89.544	0.383
pilT	Acinetobacter baylyi ADP1	40.588	91.153	0.37
pilT	Vibrio cholerae strain A1552	41.641	88.204	0.367
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.641	88.204	0.367