Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BT141_RS12200 Genome accession   NZ_LT632614
Coordinates   2672645..2673766 (-) Length   373 a.a.
NCBI ID   WP_042755108.1    Uniprot ID   -
Organism   Legionella pneumophila strain ST62 isolate ST62     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2667645..2678766
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BT141_RS12185 (H044120014_02420) - 2668527..2668964 (-) 438 WP_027225907.1 hypothetical protein -
  BT141_RS12190 (H044120014_02421) - 2669245..2670540 (+) 1296 WP_021436879.1 serine/threonine transporter -
  BT141_RS12195 (H044120014_02422) ankK 2670633..2672558 (-) 1926 WP_042755109.1 Dot/Icm T4SS effector AnkK/LegA5 -
  BT141_RS12200 (H044120014_02423) pilU 2672645..2673766 (-) 1122 WP_042755108.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BT141_RS12205 (H044120014_02424) - 2673856..2675784 (-) 1929 WP_042755107.1 L-gulono-gamma-lactone oxidase -
  BT141_RS12210 (H044120014_02425) - 2676071..2676907 (-) 837 WP_042755106.1 phytanoyl-CoA dioxygenase family protein -
  BT141_RS12215 (H044120014_02426) radA 2676904..2678259 (-) 1356 WP_042755105.1 DNA repair protein RadA -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41673.95 Da        Isoelectric Point: 5.4724

>NTDB_id=1145506 BT141_RS12200 WP_042755108.1 2672645..2673766(-) (pilU) [Legionella pneumophila strain ST62 isolate ST62]
MDITPFFKLMVDKGASDLFFSVGAPPNIKIEGITSPIGQVPIKSNQMAEIVLSIMNDAQQKEFESKMELNMAFSLAGVGR
FRINLFRQRGEVAMVVRYIKGEIPSIEDLNLPQILKSLVLELRGLVLVVGSTGSGKSTTLAAMINHRNENHRGHILTIED
PIEYIHQHKKSIVDQREIGIDTLSYDNALKNAMREAPDVILIGEIRDLNSMKHAIAYAETGHLCISTLHANNANQALDRV
INFFPDEARQQILLDLSLNLKAIISLRLIPGVDNQRVAAVEILINSPYISDLIEKGKIDDIKEVMARSTEQGMQTFDQAL
FNLYKEGKITKENAIKYADSKNNVGLQIRLGEEKSFNIQDENLQIQPDDIDKA

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=1145506 BT141_RS12200 WP_042755108.1 2672645..2673766(-) (pilU) [Legionella pneumophila strain ST62 isolate ST62]
ATGGATATTACTCCTTTTTTTAAGCTCATGGTCGATAAAGGTGCCTCAGATTTGTTTTTCAGTGTCGGGGCACCACCAAA
TATTAAAATCGAGGGCATCACATCACCTATTGGCCAGGTTCCTATCAAATCGAATCAAATGGCAGAAATTGTTTTATCTA
TTATGAATGACGCACAGCAAAAAGAATTTGAGTCTAAAATGGAACTCAATATGGCTTTTTCATTAGCTGGAGTAGGGCGT
TTCCGAATCAATTTATTCAGGCAACGTGGCGAAGTGGCTATGGTGGTGCGTTACATCAAGGGCGAAATACCTTCTATAGA
AGATTTAAATCTTCCTCAAATTTTAAAGTCTCTGGTCTTGGAGTTGCGAGGTTTAGTATTGGTTGTTGGGTCCACGGGAT
CTGGTAAATCAACAACTCTGGCCGCGATGATCAACCACCGTAATGAAAATCACAGAGGGCATATTTTAACAATAGAGGAT
CCTATTGAATATATACATCAACATAAAAAATCAATAGTTGATCAACGAGAGATAGGTATTGATACTCTAAGCTATGACAA
TGCATTGAAAAACGCAATGAGAGAAGCGCCTGATGTGATATTAATTGGTGAAATTCGTGATCTTAACTCAATGAAACATG
CGATAGCTTATGCTGAAACAGGACATTTGTGCATTTCTACTTTGCATGCCAATAACGCCAATCAGGCTCTGGATAGAGTG
ATTAATTTTTTCCCCGATGAGGCAAGACAACAGATATTATTAGATTTATCTCTTAATTTAAAAGCAATCATTTCATTACG
TTTGATACCAGGAGTTGATAATCAACGTGTCGCAGCGGTGGAGATCTTGATTAACTCTCCTTACATTTCCGATTTGATTG
AAAAAGGGAAGATAGACGATATTAAAGAAGTCATGGCAAGAAGCACGGAACAAGGCATGCAGACTTTCGATCAGGCTTTA
TTTAATTTATATAAAGAAGGAAAAATTACCAAAGAAAATGCGATTAAGTATGCTGATTCAAAAAACAATGTGGGTTTACA
AATTCGATTGGGCGAAGAAAAAAGCTTTAATATTCAAGATGAGAATTTACAAATACAACCCGATGATATTGACAAGGCAT
GA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(43-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.367

94.906

0.525
  pilU Acinetobacter baylyi ADP1

52.958

95.174

0.504
  pilU Vibrio cholerae strain A1552

48.286

93.834

0.453
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.428

90.885

0.413
  pilT Legionella pneumophila strain ERS1305867

43.275

91.689

0.397
  pilT Legionella pneumophila strain Lp02

43.275

91.689

0.397
  pilT Neisseria meningitidis 8013

42.363

93.029

0.394
  pilT Neisseria gonorrhoeae MS11

42.363

93.029

0.394
  pilT Pseudomonas aeruginosa PAK

42.566

91.957

0.391
  pilT Pseudomonas stutzeri DSM 10701

41.983

91.957

0.386
  pilT Acinetobacter baumannii strain A118

43.114

89.544

0.386
  pilT Acinetobacter nosocomialis M2

43.114

89.544

0.386
  pilT Acinetobacter baumannii D1279779

43.114

89.544

0.386
  pilT Acinetobacter baylyi ADP1

40.882

91.153

0.373
  pilT Vibrio cholerae strain A1552

41.945

88.204

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.945

88.204

0.37

Multiple sequence alignment