Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   J151_RS18745 Genome accession   NZ_CP006857
Coordinates   4278506..4279639 (+) Length   377 a.a.
NCBI ID   WP_003484093.1    Uniprot ID   A0AAI7ZI10
Organism   Xanthomonas citri subsp. citri A306     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4273506..4284639
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  J151_RS18720 (J151_03788) - 4273662..4274858 (-) 1197 WP_011052390.1 cystathionine gamma-synthase -
  J151_RS18725 (J151_03789) - 4274958..4276328 (-) 1371 WP_011052391.1 pyridoxal-phosphate dependent enzyme -
  J151_RS18730 (J151_03790) - 4276567..4276785 (-) 219 WP_003484103.1 YdcH family protein -
  J151_RS18735 (J151_03791) - 4277137..4278027 (-) 891 WP_011052392.1 membrane protein -
  J151_RS18740 (J151_03792) - 4278024..4278407 (-) 384 WP_005914753.1 DUF4398 domain-containing protein -
  J151_RS18745 (J151_03793) pilU 4278506..4279639 (+) 1134 WP_003484093.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  J151_RS18755 (J151_03794) maiA 4280005..4280667 (-) 663 WP_011052393.1 maleylacetoacetate isomerase -
  J151_RS18760 (J151_03795) - 4280699..4281688 (-) 990 WP_005920827.1 fumarylacetoacetate hydrolase family protein -
  J151_RS18765 (J151_03796) - 4282071..4283453 (+) 1383 WP_011052394.1 DEAD/DEAH box helicase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41763.12 Da        Isoelectric Point: 6.1401

>NTDB_id=114460 J151_RS18745 WP_003484093.1 4278506..4279639(+) (pilU) [Xanthomonas citri subsp. citri A306]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKGVDGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKSGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYEAAAAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=114460 J151_RS18745 WP_003484093.1 4278506..4279639(+) (pilU) [Xanthomonas citri subsp. citri A306]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTTTACCCGCTCGGCAATACGGGGTTGCCGCCGGGGATGGTCAAGAAAATCGCTTACTCGT
TGATGGACGAAGGCCAGGTACCGCAGTTCGAGCGCGAGCTCGAGCTCAACATGGCCATCGCCCTGCCGGACGCCGGCCGC
TTCCGCGTGAACGTATTCAAGCAGCGCGGCGAGGTGGGCATGGTGATTCGCGCCATTCGCAGCCGGATTCCCAGCATCGA
AGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGTTCGACCGGCT
CGGGCAAGTCGACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAATATCTGCACAAACACAAGATGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGACACCCACGCCTTCCAGAG
CGCGCTGAAGAACGCCATGCGCGAGGCGCCGGACGTGATTCTGATCGGCGAGATCCTGGACGCCGAAACCATGGAGGCGG
CGATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACTCCAACAATGCCGACCAGACCATCGAGCGCATC
CTCAATTTCTTCCCGGAAAGCGCGCACAAGAACGTGTTGATGAACCTGGCGCTGAACCTGCGCGCGGTCATTTCCCAGCG
TTTGGTCAAGGGCGTGGACGGCCGTCGTCGACCGGCCACCGAAGTGCTGCTCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAAGTGCACGAGGTCAAGCAGGCGATGGAAGAGTCGCTGGAAGAAGGCATGCAGACCTTCGACCAATGCCTG
TTCCGGATGGTGAAGTCGGGCGAAATCGAGCAGGAAGAAGCGCTGCGCGCTGCCGACTCGCGCGACGGCCTGGCCCTGAA
GTTCCGTCTTTCCGAAGGCGGCTCCGGCGAGCACGACCCGTACGCGGACTACGAAGCGGCCGCGGCACCGAAGATCAGCC
ACGGGTTCATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

95.225

0.496
  pilU Vibrio cholerae strain A1552

49.444

95.491

0.472
  pilU Acinetobacter baylyi ADP1

49.718

93.899

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.241

100

0.448
  pilT Pseudomonas aeruginosa PAK

43.195

89.655

0.387
  pilT Acinetobacter baumannii strain A118

41.716

89.655

0.374
  pilT Acinetobacter nosocomialis M2

41.716

89.655

0.374
  pilT Acinetobacter baumannii D1279779

41.716

89.655

0.374
  pilT Pseudomonas stutzeri DSM 10701

41.916

88.594

0.371
  pilT Acinetobacter baylyi ADP1

41.176

90.186

0.371
  pilT Legionella pneumophila strain Lp02

41.124

89.655

0.369
  pilT Legionella pneumophila strain ERS1305867

41.124

89.655

0.369
  pilT Vibrio cholerae strain A1552

41.441

88.329

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

88.329

0.366
  pilT Neisseria gonorrhoeae MS11

39.481

92.042

0.363
  pilT Neisseria meningitidis 8013

39.481

92.042

0.363

Multiple sequence alignment