Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   CBM981_RS11095 Genome accession   NZ_LT578417
Coordinates   2186642..2187721 (+) Length   359 a.a.
NCBI ID   WP_087068453.1    Uniprot ID   A0A182ARM2
Organism   Cyanobium sp. NIES-981     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2181642..2192721
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CBM981_RS11075 (CBM981_2396) - 2182612..2182866 (-) 255 WP_087068450.1 sulfurtransferase TusA family protein -
  CBM981_RS11080 (CBM981_2397) dnaJ 2182871..2183995 (-) 1125 WP_087068451.1 molecular chaperone DnaJ -
  CBM981_RS11085 (CBM981_2398) grpE 2183999..2184736 (-) 738 WP_157665421.1 nucleotide exchange factor GrpE -
  CBM981_RS11090 (CBM981_2399) pilB/pilB1 2184822..2186597 (+) 1776 WP_172820879.1 GspE/PulE family protein Machinery gene
  CBM981_RS11095 (CBM981_2400) pilT 2186642..2187721 (+) 1080 WP_087068453.1 type IV pilus twitching motility protein PilT Machinery gene
  CBM981_RS11100 (CBM981_2401) - 2187739..2188995 (+) 1257 WP_087068454.1 type II secretion system F family protein -
  CBM981_RS11105 (CBM981_2402) - 2189043..2190311 (-) 1269 WP_087068455.1 NAD(P)/FAD-dependent oxidoreductase -
  CBM981_RS11110 (CBM981_2403) dusA 2190406..2191443 (+) 1038 WP_087068456.1 tRNA dihydrouridine(20/20a) synthase DusA -
  CBM981_RS11115 (CBM981_2404) - 2191475..2191993 (-) 519 WP_087068457.1 RNA-binding protein -

Sequence

Protein


Download         Length: 359 a.a.        Molecular weight: 39832.56 Da        Isoelectric Point: 5.8380

>NTDB_id=1143945 CBM981_RS11095 WP_087068453.1 2186642..2187721(+) (pilT) [Cyanobium sp. NIES-981]
MAVIIEDLMNQLVADGGSDLHLSAGLPPYGRFNDQLRPILDDRLEEEACNRLIFSLLNNAQRKQLEQNWELDCSYGLKGV
ARFRVNVYRQRGTYAACLRALGNDIPTLDALHLPPIVEEISRSPKGLVLVTGPTGSGKTTTLAAVLDHINHTRSEHILTI
EDPIEFTYRSDKSVIHQRQLNDDTRSFANALRAALREDPDVILVGELRDLETIQLAITAAETGHLVFATLHTSSAAQTVD
RMVDVFPAGQQTQIRVQLSTSLVAVFAQTLCKRHNPVPGEYGRVMAQEIMINTPAIANLIREGKTAQLYSQMQMGGQMSM
QTLEKSLADLVMSNQILMDEALTKTGKPEELKRLLNQLN

Nucleotide


Download         Length: 1080 bp        

>NTDB_id=1143945 CBM981_RS11095 WP_087068453.1 2186642..2187721(+) (pilT) [Cyanobium sp. NIES-981]
ATGGCCGTCATTATTGAAGATCTGATGAACCAGCTGGTTGCCGACGGCGGCAGTGATCTTCATCTCAGCGCTGGTCTTCC
CCCCTATGGCCGCTTCAATGACCAGCTGCGGCCCATCCTCGACGACAGACTGGAAGAGGAAGCCTGCAACAGGCTGATCT
TCTCGCTGCTGAACAATGCTCAACGCAAGCAGCTGGAGCAGAACTGGGAGCTGGACTGTTCCTACGGGCTCAAGGGAGTT
GCGCGGTTCAGGGTCAATGTCTACCGCCAGCGTGGCACCTATGCAGCCTGTCTACGGGCCCTTGGCAATGATATTCCAAC
CCTGGACGCCCTTCACCTTCCACCGATTGTGGAGGAAATCAGCCGCTCTCCCAAGGGTTTGGTTTTGGTAACCGGCCCTA
CTGGGTCAGGGAAAACCACCACACTTGCGGCTGTGCTCGATCATATCAATCACACCCGTTCCGAGCATATTCTCACCATT
GAAGACCCGATTGAATTCACCTACCGCTCCGATAAGAGCGTGATCCATCAGCGTCAACTCAATGACGACACACGCAGCTT
TGCCAATGCCTTGCGCGCCGCCCTGCGGGAAGATCCTGATGTGATTCTGGTGGGTGAGCTCCGGGATCTTGAAACCATCC
AGCTGGCCATCACAGCAGCGGAAACAGGCCACCTCGTGTTCGCCACCCTGCACACCAGCTCCGCCGCCCAGACGGTGGAC
CGGATGGTGGATGTATTTCCGGCTGGTCAGCAGACCCAGATCCGGGTACAACTGAGCACGAGCCTCGTGGCTGTGTTCGC
CCAGACGCTCTGCAAACGTCACAATCCCGTTCCCGGAGAGTACGGTCGCGTGATGGCGCAGGAGATCATGATCAACACCC
CTGCCATCGCCAACCTGATCCGGGAAGGTAAGACGGCCCAGCTTTACTCCCAGATGCAGATGGGCGGCCAGATGTCGATG
CAGACCCTGGAGAAATCCCTTGCTGACCTGGTCATGAGCAACCAGATTCTCATGGATGAAGCTCTCACCAAGACAGGCAA
ACCCGAAGAGCTCAAGCGCCTGCTGAACCAGCTCAACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(115-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A182ARM2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

52.161

96.657

0.504
  pilT Pseudomonas stutzeri DSM 10701

51.873

96.657

0.501
  pilT Acinetobacter baumannii D1279779

51.297

96.657

0.496
  pilT Acinetobacter baumannii strain A118

51.297

96.657

0.496
  pilT Acinetobacter nosocomialis M2

51.297

96.657

0.496
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.059

94.708

0.493
  pilT Vibrio cholerae strain A1552

52.059

94.708

0.493
  pilT Legionella pneumophila strain Lp02

52.711

92.479

0.487
  pilT Legionella pneumophila strain ERS1305867

52.711

92.479

0.487
  pilT Acinetobacter baylyi ADP1

50.432

96.657

0.487
  pilT Neisseria meningitidis 8013

52.599

91.086

0.479
  pilT Neisseria gonorrhoeae MS11

52.294

91.086

0.476
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.295

98.05

0.474
  pilU Acinetobacter baylyi ADP1

37.536

97.214

0.365
  pilU Pseudomonas stutzeri DSM 10701

37.322

97.772

0.365

Multiple sequence alignment