Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   CBM981_RS11090 Genome accession   NZ_LT578417
Coordinates   2184822..2186597 (+) Length   591 a.a.
NCBI ID   WP_172820879.1    Uniprot ID   A0A182ARC0
Organism   Cyanobium sp. NIES-981     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2179822..2191597
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CBM981_RS11060 (CBM981_2393) murB 2180216..2181115 (+) 900 WP_087068447.1 UDP-N-acetylmuramate dehydrogenase -
  CBM981_RS11065 (CBM981_2394) - 2181180..2181521 (+) 342 WP_087068448.1 YbaB/EbfC family nucleoid-associated protein -
  CBM981_RS11070 (CBM981_2395) rsgA 2181528..2182622 (-) 1095 WP_087068449.1 ribosome small subunit-dependent GTPase A -
  CBM981_RS11075 (CBM981_2396) - 2182612..2182866 (-) 255 WP_087068450.1 sulfurtransferase TusA family protein -
  CBM981_RS11080 (CBM981_2397) dnaJ 2182871..2183995 (-) 1125 WP_087068451.1 molecular chaperone DnaJ -
  CBM981_RS11085 (CBM981_2398) grpE 2183999..2184736 (-) 738 WP_157665421.1 nucleotide exchange factor GrpE -
  CBM981_RS11090 (CBM981_2399) pilB/pilB1 2184822..2186597 (+) 1776 WP_172820879.1 GspE/PulE family protein Machinery gene
  CBM981_RS11095 (CBM981_2400) pilT 2186642..2187721 (+) 1080 WP_087068453.1 type IV pilus twitching motility protein PilT Machinery gene
  CBM981_RS11100 (CBM981_2401) - 2187739..2188995 (+) 1257 WP_087068454.1 type II secretion system F family protein -
  CBM981_RS11105 (CBM981_2402) - 2189043..2190311 (-) 1269 WP_087068455.1 NAD(P)/FAD-dependent oxidoreductase -
  CBM981_RS11110 (CBM981_2403) dusA 2190406..2191443 (+) 1038 WP_087068456.1 tRNA dihydrouridine(20/20a) synthase DusA -

Sequence

Protein


Download         Length: 591 a.a.        Molecular weight: 64739.80 Da        Isoelectric Point: 5.4570

>NTDB_id=1143944 CBM981_RS11090 WP_172820879.1 2184822..2186597(+) (pilB/pilB1) [Cyanobium sp. NIES-981]
MPQTPSRPVPEALSREQQRLEAELLLGKPVLSEDQLLRQRVDDPVLKADEWRELGACPVANHGDELVVAVPIHFGAESRQ
AVARTLESRGTSPKLVLALAADIAAAQERIGAGPTRQGQARSLTRDLDIPTADGELQEISDLEDLEVSEAEASSSANASP
IVSLVDRILIEALTTGASDIHIEPQDSSMTIRFRIDGVLQQHFEVLPKSLVPAVTSRVKIMADLDIAERRMPQDGRIRRM
FRGRKMDFRVSTLPTRNGEKVVLRLLDSGDTQLGLDSLITDPGARQSLRELGARPYGMILVTGPTGSGKSTTLYALLSER
NDQHINISTVEDPIEYTIAGITQTQVNREKGLDFSTALRAFMRQDPDVLLVGETRDLETAKTAIEAALTGHLVMTTLHCN
DAASAIARLDEMGVEPFMVSASLLGIVSQRLLRRVCLHCRISYRPSPEELSRFGLLSSMETDVTFFRARSSKPGHGDYCS
ACNGSGYKGRVGVYEVLRMNDTMAAAIAKRQTTDVIRRLALESGMKTLLGYGLQLVREGLTTLTEVERMLLTDSGLESER
RSKALNSLTCTGCGAGLHDDWLQCPYCLTER

Nucleotide


Download         Length: 1776 bp        

>NTDB_id=1143944 CBM981_RS11090 WP_172820879.1 2184822..2186597(+) (pilB/pilB1) [Cyanobium sp. NIES-981]
ATGCCCCAGACCCCCTCCAGACCCGTCCCTGAAGCGCTCAGCCGCGAGCAGCAGCGTCTCGAGGCGGAGTTGCTGCTGGG
GAAGCCCGTGCTGAGCGAGGACCAGCTGCTCCGGCAGCGGGTCGACGACCCGGTGCTCAAAGCCGACGAGTGGCGCGAGC
TCGGCGCCTGTCCGGTGGCCAACCATGGGGACGAGCTGGTGGTGGCGGTGCCGATCCACTTCGGGGCCGAATCCCGCCAG
GCCGTGGCCCGCACACTCGAATCGAGGGGGACGAGCCCGAAGCTGGTGCTGGCCCTGGCGGCTGACATCGCGGCAGCCCA
GGAGCGGATCGGTGCGGGCCCCACGCGGCAGGGCCAGGCCCGCTCCCTCACCAGGGACCTCGACATCCCGACGGCCGACG
GGGAACTGCAGGAGATCAGCGACCTTGAGGATCTGGAGGTCAGCGAAGCCGAGGCGAGTTCAAGCGCCAACGCCTCACCG
ATCGTGAGCCTGGTGGACAGGATTCTGATCGAGGCCCTCACCACCGGTGCGAGCGACATCCACATCGAACCGCAGGACAG
CAGCATGACGATCCGCTTCAGGATCGACGGTGTGCTGCAGCAACACTTCGAAGTCCTGCCGAAAAGCCTGGTACCGGCGG
TCACATCGCGGGTCAAGATCATGGCCGATCTTGACATCGCTGAACGCAGGATGCCCCAGGACGGGCGCATCCGACGGATG
TTCCGGGGGCGGAAGATGGATTTCCGCGTCAGCACGCTCCCGACCCGCAATGGCGAGAAAGTCGTGCTGCGCCTGCTCGA
CAGCGGCGACACCCAGCTTGGCCTCGACTCTTTGATCACAGACCCAGGGGCCCGGCAGAGCCTGCGGGAACTGGGGGCGC
GTCCCTACGGCATGATCCTGGTGACCGGACCGACCGGCTCGGGTAAGTCCACCACCTTGTACGCCCTGCTGTCGGAACGC
AACGACCAGCACATCAACATCTCCACGGTGGAGGACCCGATTGAATACACCATCGCGGGAATCACCCAGACCCAGGTGAA
CCGGGAGAAGGGTCTGGACTTCTCCACGGCCCTGCGCGCTTTCATGCGGCAGGATCCTGATGTGCTCCTGGTGGGTGAGA
CCCGCGACCTCGAGACCGCCAAGACGGCCATCGAAGCAGCCCTCACCGGCCACCTGGTGATGACCACCCTGCACTGCAAC
GACGCGGCCAGTGCGATCGCCCGTCTCGATGAGATGGGTGTGGAGCCGTTCATGGTGAGTGCCTCCCTGCTCGGAATCGT
GTCCCAGCGGCTGCTGCGGCGGGTCTGCCTTCATTGCCGGATCAGTTATCGCCCCAGCCCGGAAGAGTTGAGCCGCTTCG
GATTGCTCAGCAGCATGGAAACCGATGTGACCTTCTTCCGCGCCAGAAGCAGCAAGCCCGGCCATGGAGACTACTGTTCA
GCCTGCAATGGCAGCGGCTACAAGGGACGGGTTGGCGTGTACGAAGTGCTGCGGATGAATGACACCATGGCAGCGGCGAT
CGCGAAGCGCCAGACCACCGATGTGATCCGCCGCCTGGCCCTGGAGAGCGGCATGAAAACCCTGCTCGGCTACGGCCTTC
AACTGGTCAGGGAGGGGCTCACGACCCTGACGGAAGTGGAGCGCATGCTTCTCACGGACTCAGGCCTGGAAAGTGAGCGC
CGTTCCAAGGCGCTGAACTCGCTCACCTGCACCGGCTGCGGAGCAGGGCTGCATGACGACTGGCTGCAGTGCCCCTATTG
CCTCACCGAGCGCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(13-114)

Type II/IV secretion system protein (T2SSE)

(168-434)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A182ARC0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

60.404

83.756

0.506
  pilB Vibrio cholerae strain A1552

43.59

85.787

0.374

Multiple sequence alignment