Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   A8U35_RS03705 Genome accession   NZ_LT571437
Coordinates   779316..780296 (+) Length   326 a.a.
NCBI ID   WP_001055643.1    Uniprot ID   A0A3V2Y5P8
Organism   Salmonella enterica subsp. enterica serovar Java strain NCTC5706     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 774316..785296
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8U35_RS03675 (SAMEA3468845_00757) - 774893..775900 (+) 1008 WP_023209131.1 DUF1202 domain-containing protein -
  A8U35_RS03680 (SAMEA3468845_00758) hemW 775992..777128 (-) 1137 WP_023209130.1 radical SAM family heme chaperone HemW -
  A8U35_RS03685 (SAMEA3468845_00759) - 777121..777714 (-) 594 WP_001174769.1 XTP/dITP diphosphatase -
  A8U35_RS03690 (SAMEA3468845_00760) yggU 777722..778012 (-) 291 WP_001277205.1 DUF167 family protein YggU -
  A8U35_RS03695 (SAMEA3468845_00761) - 778009..778575 (-) 567 WP_001094848.1 YggT family protein -
  A8U35_RS03700 (SAMEA3468845_00762) - 778594..779298 (-) 705 WP_023209129.1 YggS family pyridoxal phosphate-dependent enzyme -
  A8U35_RS03705 (SAMEA3468845_00763) pilT 779316..780296 (+) 981 WP_001055643.1 type IV pilus twitching motility protein PilT Machinery gene
  A8U35_RS03710 (SAMEA3468845_00764) - 780429..781115 (+) 687 WP_000098324.1 IclR family transcriptional regulator domain-containing protein -
  A8U35_RS03715 (SAMEA3468845_00765) ruvX 781162..781578 (-) 417 WP_000017096.1 Holliday junction resolvase RuvX -
  A8U35_RS03720 (SAMEA3468845_00766) - 781578..782141 (-) 564 WP_001053167.1 YqgE/AlgH family protein -
  A8U35_RS03725 (SAMEA3468845_00767) gshB 782357..783304 (-) 948 WP_000593248.1 glutathione synthase -
  A8U35_RS03730 (SAMEA3468845_00768) rsmE 783324..784055 (-) 732 WP_001675718.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  A8U35_RS03735 (SAMEA3468845_00769) endA 784132..784839 (-) 708 WP_023209128.1 deoxyribonuclease I -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 36153.34 Da        Isoelectric Point: 7.5355

>NTDB_id=1143819 A8U35_RS03705 WP_001055643.1 779316..780296(+) (pilT) [Salmonella enterica subsp. enterica serovar Java strain NCTC5706]
MNMEEIVTLSVKHNVSDLHLCNAWPARWRKQGRMENAPFTAPDVDRLLLDWLNDAQQYQWRTHGQLDFAVSLSGTRRLRA
SAFTHQQGTSLALRLLPERCPDLAEIQTPPIVPALLASENGLILVTGATGCGKSTTLAAMVGHLNQHADKHILTLEDPIE
YRYTSKRCLIQQREIGQHCATFAAGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKEPVRSQLAGSLRAVLSQKLEVDRQDGRVALFELLINTPATGNLIREGKLHQLAHVIQTGQQQGMMTFAQSAQWR
QVQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1143819 A8U35_RS03705 WP_001055643.1 779316..780296(+) (pilT) [Salmonella enterica subsp. enterica serovar Java strain NCTC5706]
ATGAATATGGAAGAAATTGTGACGCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGTAACGCCTGGCCTGCGCG
ATGGCGCAAACAAGGACGCATGGAGAACGCGCCTTTTACCGCGCCTGATGTCGACAGGCTTCTGCTTGATTGGCTTAATG
ATGCGCAACAATATCAGTGGCGAACGCACGGCCAGCTTGATTTCGCCGTCTCGCTGTCTGGCACGCGGCGCCTGCGCGCC
AGTGCGTTCACACATCAACAGGGAACGTCGCTGGCGCTACGGCTATTGCCCGAGCGTTGTCCTGATCTGGCGGAAATCCA
GACGCCGCCGATAGTACCCGCACTGCTTGCCAGCGAGAACGGACTGATTCTGGTCACTGGCGCTACTGGCTGCGGGAAAT
CCACTACGCTGGCGGCGATGGTGGGACATCTTAATCAACATGCCGATAAACACATCCTGACGCTGGAAGATCCTATTGAG
TACCGTTACACCAGCAAGCGTTGCCTGATACAGCAGCGGGAAATCGGCCAGCATTGCGCCACTTTTGCCGCCGGGCTGCG
CGCGGCGTTACGTGAAGATCCCGATGTCATTTTACTGGGAGAGCTGCGTGACAGCGAGACCATTCGCCTGGCGCTGACGG
CGGCGGAAACGGGGCATCTGGTACTGGCAACCCTGCATACTCGCGGTGCGGCGCAGGCGGTGGAAAGATTAGTGGATAGT
TTTCCGGCGCAGGAAAAAGAACCGGTACGCAGCCAACTGGCGGGTAGTCTGCGCGCCGTACTATCACAAAAACTGGAGGT
CGACAGACAGGATGGGCGCGTGGCGTTGTTTGAATTACTGATTAATACGCCAGCCACAGGGAATTTGATCCGTGAAGGCA
AACTCCATCAGTTGGCCCACGTGATACAAACCGGGCAGCAGCAGGGGATGATGACATTCGCCCAGAGCGCGCAATGGCGT
CAGGTGCAGGGACGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3V2Y5P8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

48.318

100

0.485
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.318

100

0.485
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baumannii D1279779

45.26

100

0.454
  pilT Acinetobacter baumannii strain A118

45.26

100

0.454
  pilT Acinetobacter nosocomialis M2

46.082

97.853

0.451
  pilT Acinetobacter baylyi ADP1

44.954

100

0.451
  pilT Pseudomonas stutzeri DSM 10701

45.886

96.933

0.445
  pilT Pseudomonas aeruginosa PAK

45.886

96.933

0.445
  pilT Legionella pneumophila strain ERS1305867

44.654

97.546

0.436
  pilT Legionella pneumophila strain Lp02

44.654

97.546

0.436
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

38.558

97.853

0.377
  pilU Pseudomonas stutzeri DSM 10701

36.556

100

0.371
  pilU Acinetobacter baylyi ADP1

36.842

99.08

0.365

Multiple sequence alignment