Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PPRCHA0_RS29625 Genome accession   NZ_LS999205
Coordinates   6439975..6441009 (-) Length   344 a.a.
NCBI ID   WP_015637368.1    Uniprot ID   A0A2C9EV78
Organism   Pseudomonas protegens CHA0     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6434975..6446009
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PPRCHA0_RS29595 (PPRCHA0_5734) ruvX 6435117..6435554 (+) 438 WP_015637366.1 Holliday junction resolvase RuvX -
  PPRCHA0_RS29600 (PPRCHA0_5735) pyrR 6435670..6436176 (+) 507 WP_011064006.1 bifunctional pyr operon transcriptional regulator/uracil phosphoribosyltransferase PyrR -
  PPRCHA0_RS29605 (PPRCHA0_5736) - 6436202..6437206 (+) 1005 WP_011064007.1 aspartate carbamoyltransferase catalytic subunit -
  PPRCHA0_RS29610 (PPRCHA0_5737) - 6437203..6438474 (+) 1272 WP_011064008.1 dihydroorotase -
  PPRCHA0_RS29615 (PPRCHA0_5738) - 6438602..6439021 (-) 420 WP_011064009.1 TM2 domain-containing protein -
  PPRCHA0_RS29620 (PPRCHA0_5739) - 6439273..6439896 (+) 624 WP_015637367.1 C40 family peptidase -
  PPRCHA0_RS29625 (PPRCHA0_5740) pilT 6439975..6441009 (-) 1035 WP_015637368.1 type IV pilus twitching motility protein PilT Machinery gene
  PPRCHA0_RS29630 (PPRCHA0_5741) - 6441200..6441898 (+) 699 WP_011064012.1 YggS family pyridoxal phosphate-dependent enzyme -
  PPRCHA0_RS29635 (PPRCHA0_5742) proC 6441933..6442751 (+) 819 WP_015637369.1 pyrroline-5-carboxylate reductase -
  PPRCHA0_RS29640 (PPRCHA0_5743) - 6442762..6443352 (+) 591 WP_011064014.1 YggT family protein -
  PPRCHA0_RS29645 (PPRCHA0_5744) - 6443367..6443660 (+) 294 WP_011064015.1 DUF167 domain-containing protein -
  PPRCHA0_RS29650 (PPRCHA0_5745) - 6443824..6444963 (+) 1140 WP_041752663.1 homoserine O-acetyltransferase -
  PPRCHA0_RS29655 (PPRCHA0_5746) metW 6444971..6445591 (+) 621 WP_011064017.1 methionine biosynthesis protein MetW -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 37335.09 Da        Isoelectric Point: 6.8889

>NTDB_id=1143442 PPRCHA0_RS29625 WP_015637368.1 6439975..6441009(-) (pilT) [Pseudomonas protegens CHA0]
MDITELLRLSASRGASDLHLSAGLAPMLRIDGDIRPLPGPALEGPEVLALIHELMSEAQRQAFAACNDLDFAHELPGVAR
FRVNAFRHARGAGAVLRLIPARVQSLEELGLGEVFRQITEAPRGLVLVTGPTGSGKSTTLAAMIDHLNQRRQQHILTIED
PIEFVHPQKNCLVHQREVQRDTQSFATALRAALREDPDVILLGELRDLETIRLALTAAETGHLVLATLHTSSAAKTIDRL
VDVFPGEEKALVRSMLAESLQAVVAQVLVKKVGGGRVAAHEIMLGSPAIRNLIREDKVAQMYSAIQTGGAQGMRTLDMSL
KALLGQGLISRDQAREQARMPESF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=1143442 PPRCHA0_RS29625 WP_015637368.1 6439975..6441009(-) (pilT) [Pseudomonas protegens CHA0]
ATGGACATCACCGAACTCTTGCGCTTGAGCGCTTCCCGGGGCGCTTCGGACCTGCATCTGTCCGCCGGGCTGGCGCCGAT
GTTGCGCATCGATGGCGATATTCGCCCGCTGCCGGGCCCGGCCCTGGAAGGGCCAGAGGTGCTGGCGCTGATCCATGAAC
TGATGAGCGAAGCTCAGCGCCAGGCATTCGCCGCCTGCAATGACCTGGATTTCGCCCATGAACTGCCAGGTGTGGCGCGG
TTTCGGGTCAATGCCTTCCGGCACGCCCGGGGCGCCGGTGCGGTGCTGCGCCTGATTCCCGCGCGGGTACAGAGCCTGGA
GGAGCTGGGGCTGGGGGAAGTGTTTCGGCAGATTACCGAGGCGCCCCGCGGGCTGGTGCTGGTGACCGGGCCCACCGGCA
GCGGCAAGTCCACCACACTGGCGGCGATGATCGACCACCTGAACCAGCGCCGCCAGCAGCACATCCTGACCATCGAAGAC
CCTATCGAATTCGTGCATCCGCAGAAAAACTGCCTGGTGCACCAGCGCGAAGTGCAGCGCGATACCCAGAGCTTCGCCAC
GGCCTTGCGTGCGGCGCTGCGGGAAGATCCCGACGTGATCCTGCTGGGCGAACTGCGGGACCTGGAAACCATCCGCCTGG
CCCTGACCGCCGCGGAAACCGGGCACTTGGTACTGGCCACCTTGCACACCTCGTCGGCGGCCAAGACCATCGACCGGCTG
GTGGACGTGTTTCCAGGGGAGGAAAAAGCCCTGGTGCGCTCGATGCTGGCCGAGTCGCTGCAGGCAGTGGTCGCGCAAGT
GCTGGTGAAGAAGGTGGGCGGTGGCCGGGTAGCGGCGCACGAGATCATGCTGGGCAGCCCGGCGATCCGTAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCGGCGATCCAGACGGGCGGCGCCCAGGGCATGCGCACCCTGGACATGAGCCTC
AAGGCATTGCTCGGGCAGGGGTTGATCAGCCGCGATCAGGCGCGGGAGCAGGCGCGGATGCCGGAGAGCTTTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(14-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2C9EV78

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

70.93

100

0.709
  pilT Pseudomonas stutzeri DSM 10701

70.93

100

0.709
  pilT Acinetobacter nosocomialis M2

66.279

100

0.663
  pilT Acinetobacter baumannii D1279779

66.279

100

0.663
  pilT Acinetobacter baumannii strain A118

66.279

100

0.663
  pilT Acinetobacter baylyi ADP1

66.279

100

0.663
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

62.353

98.837

0.616
  pilT Vibrio cholerae strain A1552

62.353

98.837

0.616
  pilT Legionella pneumophila strain Lp02

62.13

98.256

0.61
  pilT Legionella pneumophila strain ERS1305867

62.13

98.256

0.61
  pilT Neisseria meningitidis 8013

58.551

100

0.587
  pilT Neisseria gonorrhoeae MS11

58.261

100

0.584
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.464

100

0.526
  pilU Vibrio cholerae strain A1552

42.09

97.384

0.41
  pilU Pseudomonas stutzeri DSM 10701

41.493

97.384

0.404
  pilU Acinetobacter baylyi ADP1

38.551

100

0.387

Multiple sequence alignment