Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   R615_RS16180 Genome accession   NZ_CP006829
Coordinates   3549109..3550143 (-) Length   344 a.a.
NCBI ID   WP_025266507.1    Uniprot ID   -
Organism   Thalassolituus oleivorans R6-15     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3544109..3555143
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R615_RS16145 (R615_16455) moaA 3544595..3545569 (+) 975 WP_025266501.1 GTP 3',8-cyclase MoaA -
  R615_RS16150 (R615_16460) - 3545571..3546155 (+) 585 WP_025266502.1 molybdenum cofactor guanylyltransferase -
  R615_RS16155 (R615_16465) moaB 3546167..3546709 (+) 543 WP_025266503.1 molybdenum cofactor biosynthesis protein B -
  R615_RS16160 (R615_16470) moaC 3546716..3547204 (+) 489 WP_025266504.1 cyclic pyranopterin monophosphate synthase MoaC -
  R615_RS16165 (R615_16475) - 3547201..3547449 (+) 249 WP_025266505.1 MoaD/ThiS family protein -
  R615_RS16170 (R615_16480) moaE 3547451..3547906 (+) 456 WP_025266506.1 molybdopterin synthase catalytic subunit MoaE -
  R615_RS16175 (R615_16485) pilU 3547981..3549096 (-) 1116 WP_015488610.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  R615_RS16180 (R615_16490) pilT 3549109..3550143 (-) 1035 WP_025266507.1 type IV pilus twitching motility protein PilT Machinery gene
  R615_RS16185 (R615_16495) - 3550303..3550989 (+) 687 WP_015488612.1 YggS family pyridoxal phosphate-dependent enzyme -
  R615_RS16190 (R615_16500) proC 3551054..3551872 (+) 819 WP_015488613.1 pyrroline-5-carboxylate reductase -
  R615_RS16195 (R615_16505) - 3551969..3552556 (+) 588 WP_025266509.1 YggT family protein -
  R615_RS16200 (R615_16510) - 3552560..3552871 (+) 312 WP_025266510.1 DUF167 family protein -
  R615_RS16205 (R615_16515) metX 3552929..3554092 (+) 1164 WP_025266511.1 homoserine O-succinyltransferase MetX -
  R615_RS16210 (R615_16520) metW 3554089..3554691 (+) 603 WP_015488617.1 methionine biosynthesis protein MetW -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 38029.59 Da        Isoelectric Point: 6.5073

>NTDB_id=114319 R615_RS16180 WP_025266507.1 3549109..3550143(-) (pilT) [Thalassolituus oleivorans R6-15]
MDITELLAFSAKQGASDLHLSADLPPMIRVDGDVRRINLPPLGHKEVHALVYDIMNDKQRKDFEEFLETDFSFEVPGVAR
FRVNAFNHNRGAGAVFRTIPSKVLTMDQLGMGQVFKDMANISRGIVLVTGPTGSGKSTTLAAMVDYINETKYNHILTVED
PIEFVHESKKSLINQREVHRDTLGFNEALRSALREDPDVILVGEMRDLETIRLALTAAETGHVVFGTLHTSSAAKTIDRI
VDVFPAEEKSMVRSMLSESLQGVVSQGLLKKNGGGRIAAHEIMIGTPAIRNLIREDKVAQMYSSIQTGASYGMQTMDQCL
QNMVGKGLISRETAREKAKMPENF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=114319 R615_RS16180 WP_025266507.1 3549109..3550143(-) (pilT) [Thalassolituus oleivorans R6-15]
ATGGATATTACCGAACTATTGGCATTCAGCGCGAAGCAAGGCGCATCCGATTTACATCTTTCAGCTGATTTACCGCCGAT
GATTCGTGTAGACGGCGATGTGCGTCGAATTAATTTGCCGCCGTTGGGTCACAAAGAAGTACACGCGCTGGTTTACGATA
TTATGAACGATAAGCAGCGTAAGGATTTTGAAGAGTTCTTAGAAACTGACTTTTCGTTTGAAGTGCCAGGCGTTGCCCGT
TTCCGTGTGAATGCCTTTAATCACAATCGCGGTGCCGGTGCAGTGTTTCGTACGATTCCATCCAAGGTTTTGACGATGGA
TCAGCTCGGTATGGGGCAAGTCTTTAAAGACATGGCCAATATTTCGCGCGGCATTGTGCTGGTGACTGGCCCTACCGGTT
CAGGTAAGTCGACGACGTTAGCGGCTATGGTGGATTACATCAATGAAACCAAATACAACCATATTTTAACGGTTGAAGAC
CCGATTGAATTCGTACACGAATCCAAGAAAAGCCTGATCAACCAGCGTGAAGTCCATCGCGATACTCTAGGTTTTAATGA
AGCGTTACGCTCGGCGTTACGTGAAGACCCGGATGTGATTCTCGTGGGTGAGATGCGCGACTTAGAAACTATTCGTTTGG
CCTTAACCGCGGCAGAAACCGGTCACGTGGTATTTGGTACTTTGCATACCAGCTCGGCCGCGAAAACCATTGACCGTATT
GTCGACGTGTTTCCGGCAGAAGAAAAATCAATGGTGCGCTCAATGTTATCCGAGTCGCTACAAGGCGTTGTGTCTCAGGG
TTTGTTAAAGAAAAACGGCGGTGGTCGAATTGCGGCTCATGAAATCATGATTGGTACACCAGCGATTCGAAATTTGATTC
GTGAAGACAAAGTGGCGCAAATGTACTCGTCGATACAGACTGGCGCGAGTTACGGTATGCAGACTATGGATCAGTGCTTG
CAAAACATGGTCGGTAAAGGATTAATATCTCGTGAGACCGCACGAGAAAAAGCCAAAATGCCAGAAAATTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

86.919

100

0.869
  pilT Pseudomonas stutzeri DSM 10701

85.756

100

0.858
  pilT Acinetobacter nosocomialis M2

82.267

100

0.823
  pilT Acinetobacter baumannii D1279779

82.267

100

0.823
  pilT Acinetobacter baumannii strain A118

82.267

100

0.823
  pilT Acinetobacter baylyi ADP1

79.07

100

0.791
  pilT Legionella pneumophila strain Lp02

74.852

98.256

0.735
  pilT Legionella pneumophila strain ERS1305867

74.852

98.256

0.735
  pilT Neisseria meningitidis 8013

67.536

100

0.677
  pilT Neisseria gonorrhoeae MS11

67.246

100

0.674
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

66.176

98.837

0.654
  pilT Vibrio cholerae strain A1552

66.176

98.837

0.654
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.043

100

0.532
  pilU Vibrio cholerae strain A1552

40.118

98.547

0.395
  pilU Pseudomonas stutzeri DSM 10701

40

97.384

0.39
  pilU Acinetobacter baylyi ADP1

38.15

100

0.384

Multiple sequence alignment