Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DQN52_RS10550 Genome accession   NZ_LS483426
Coordinates   1954702..1955742 (-) Length   346 a.a.
NCBI ID   WP_003791105.1    Uniprot ID   -
Organism   Kingella kingae strain NCTC10529     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1949702..1960742
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN52_RS10525 (NCTC10529_02050) - 1950417..1951802 (+) 1386 WP_003787560.1 GntP family permease -
  DQN52_RS10530 (NCTC10529_02051) - 1951927..1952436 (+) 510 WP_003787558.1 gluconokinase -
  DQN52_RS10535 (NCTC10529_02052) - 1952507..1952884 (-) 378 WP_003787555.1 diacylglycerol kinase -
  DQN52_RS10540 (NCTC10529_02053) - 1952881..1953324 (-) 444 WP_003787553.1 peroxiredoxin -
  DQN52_RS10545 (NCTC10529_02054) - 1953402..1954682 (-) 1281 WP_003787551.1 PilT/PilU family type 4a pilus ATPase -
  DQN52_RS10550 (NCTC10529_02055) pilT 1954702..1955742 (-) 1041 WP_003791105.1 type IV pilus twitching motility protein PilT Machinery gene
  DQN52_RS10555 (NCTC10529_02056) pseC 1955948..1957105 (-) 1158 WP_003787545.1 UDP-4-amino-4, 6-dideoxy-N-acetyl-beta-L-altrosamine transaminase -
  DQN52_RS10560 (NCTC10529_02057) - 1957218..1958393 (-) 1176 WP_003787543.1 alpha-hydroxy acid oxidase -
  DQN52_RS10565 (NCTC10529_02058) hflX 1958620..1959756 (+) 1137 WP_003787541.1 GTPase HflX -
  DQN52_RS10570 (NCTC10529_02059) - 1959761..1960291 (+) 531 WP_003787539.1 hypothetical protein -
  DQN52_RS11025 (NCTC10529_02060) - 1960304..1960471 (+) 168 WP_155114353.1 hypothetical protein -

Sequence

Protein


Download         Length: 346 a.a.        Molecular weight: 38171.87 Da        Isoelectric Point: 8.0272

>NTDB_id=1141197 DQN52_RS10550 WP_003791105.1 1954702..1955742(-) (pilT) [Kingella kingae strain NCTC10529]
MQITDLLAFGVKNKASDLHLSAGLPPMIRVHGDVRRINLPEMSADQVGNMISSIMNDLQRKNYQQNLETDFSFELPNVAR
FRVNAFNTNRGPAAVLRTIPSKVLTLDELKAPRIFQKIADTPRGLVLVTGPTGSGKSTTLAAMIDYINDNHAGHILTIED
PIEFVHQSKKCLVNQRELHEHTHSFANALKSALREDPDIILVGEMRDPETIGLALTAAETGHLVFGTLHTTSASKTVDRI
IDVFPAGEKEMVRSMLSESLRAVIAQTLMKTKDGKGRVAAHEIMVSTPAIRNLIRENKIAQMNSTIQTGQNFGMQTLDQC
LQDLVRRNIVSLDAARAKAQNPDMIN

Nucleotide


Download         Length: 1041 bp        

>NTDB_id=1141197 DQN52_RS10550 WP_003791105.1 1954702..1955742(-) (pilT) [Kingella kingae strain NCTC10529]
ATGCAAATTACCGACTTGCTGGCTTTTGGCGTAAAAAACAAAGCATCAGACTTACACCTTTCGGCTGGCTTGCCACCGAT
GATTCGCGTACACGGCGATGTGCGCCGCATCAACTTGCCCGAAATGAGCGCAGACCAAGTCGGCAACATGATTAGCTCTA
TCATGAACGACTTGCAGCGCAAAAACTATCAACAAAACTTGGAAACCGACTTTTCGTTTGAATTGCCCAACGTGGCGCGT
TTCCGTGTGAATGCGTTTAACACCAACCGCGGTCCAGCAGCCGTGTTGCGTACCATTCCAAGCAAAGTATTAACGCTGGA
CGAACTCAAAGCACCGCGCATTTTCCAAAAAATTGCCGACACACCACGCGGTTTGGTTTTGGTTACAGGGCCAACAGGTT
CGGGTAAATCCACCACCTTGGCGGCGATGATTGACTACATCAACGACAACCACGCAGGTCATATCTTGACGATTGAAGAC
CCGATTGAGTTTGTGCATCAATCCAAAAAATGCTTGGTAAACCAACGCGAATTGCACGAACACACCCACAGCTTTGCCAA
CGCACTGAAATCGGCATTGCGTGAAGACCCTGATATCATTCTGGTGGGCGAGATGCGTGACCCTGAAACCATCGGCTTGG
CATTGACGGCTGCCGAAACGGGTCACTTGGTATTCGGTACATTGCACACCACATCGGCATCAAAAACGGTCGACCGTATT
ATTGACGTGTTCCCAGCAGGCGAAAAAGAAATGGTGCGTTCTATGTTGTCGGAATCGTTGCGTGCCGTAATCGCGCAAAC
GTTGATGAAAACTAAAGACGGAAAAGGTCGTGTTGCCGCGCACGAAATCATGGTATCCACTCCTGCGATTCGTAACTTGA
TTCGTGAAAACAAAATCGCGCAAATGAACTCGACCATTCAAACAGGTCAAAACTTTGGTATGCAAACGCTGGACCAATGT
TTGCAAGACTTGGTTCGCCGCAATATTGTGTCGTTAGACGCAGCACGCGCCAAAGCACAAAACCCAGATATGATTAACTA
A

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria gonorrhoeae MS11

79.883

99.133

0.792
  pilT Neisseria meningitidis 8013

79.3

99.133

0.786
  pilT Acinetobacter nosocomialis M2

72.595

99.133

0.72
  pilT Acinetobacter baumannii D1279779

72.595

99.133

0.72
  pilT Acinetobacter baumannii strain A118

72.595

99.133

0.72
  pilT Pseudomonas stutzeri DSM 10701

72.303

99.133

0.717
  pilT Pseudomonas aeruginosa PAK

72.012

99.133

0.714
  pilT Acinetobacter baylyi ADP1

71.72

99.133

0.711
  pilT Legionella pneumophila strain Lp02

68.986

99.711

0.688
  pilT Legionella pneumophila strain ERS1305867

68.986

99.711

0.688
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

65.882

98.266

0.647
  pilT Vibrio cholerae strain A1552

65.882

98.266

0.647
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.292

98.844

0.497
  pilU Pseudomonas stutzeri DSM 10701

42.388

96.821

0.41
  pilU Vibrio cholerae strain A1552

42.769

93.931

0.402
  pilU Acinetobacter baylyi ADP1

39.826

99.422

0.396

Multiple sequence alignment