Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DQN67_RS11685 Genome accession   NZ_LS483410
Coordinates   2586280..2587401 (-) Length   373 a.a.
NCBI ID   WP_027225181.1    Uniprot ID   -
Organism   Legionella pneumophila strain NCTC11404     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2581280..2592401
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN67_RS11670 (NCTC11404_02321) - 2582161..2582598 (-) 438 WP_014844477.1 hypothetical protein -
  DQN67_RS11675 (NCTC11404_02322) - 2582879..2584174 (+) 1296 WP_027225179.1 serine/threonine transporter -
  DQN67_RS11680 (NCTC11404_02323) ankK 2584268..2586193 (-) 1926 WP_027225180.1 Dot/Icm T4SS effector AnkK/LegA5 -
  DQN67_RS11685 (NCTC11404_02324) pilU 2586280..2587401 (-) 1122 WP_027225181.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DQN67_RS11690 (NCTC11404_02325) - 2587491..2589419 (-) 1929 WP_027225182.1 L-gulono-gamma-lactone oxidase -
  DQN67_RS11695 (NCTC11404_02326) - 2589707..2590543 (-) 837 WP_027225183.1 phytanoyl-CoA dioxygenase family protein -
  DQN67_RS11700 (NCTC11404_02327) radA 2590540..2591895 (-) 1356 WP_027219255.1 DNA repair protein RadA -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41762.10 Da        Isoelectric Point: 5.4724

>NTDB_id=1140628 DQN67_RS11685 WP_027225181.1 2586280..2587401(-) (pilU) [Legionella pneumophila strain NCTC11404]
MDITPFFKLMVDKGASDLFFSVGAPPNIKIEGITSPIGLVPIKSNQMAEIVLSIMNDAQQKEFESKMELNMAFSLAGVGR
FRINLFRQRGEVAMVVRYIKGEIPSIEDLNLPQILKSLVLELRGLVLVVGSTGSGKSTTLAAMINHRNENHRGHILTIED
PIEYIHQHKKSIVDQREIGIDTLSYDNALKNAMREAPDVILIGEIRDLNSMKHAIAYAETGHLCISTLHANNANQALDRV
INFFPDEARQQILLDLSLNLKAIISLRLIPGVDNQRVAAVEILINSPYISDLIEKGKIDDIKEVMARSTEQGMQTFDQAL
FNLYKEGKITKENAIKYADSKNNVGLQIRLGEEKNFNIQDENLQIQPDDIDKF

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=1140628 DQN67_RS11685 WP_027225181.1 2586280..2587401(-) (pilU) [Legionella pneumophila strain NCTC11404]
ATGGATATTACTCCTTTTTTTAAGCTCATGGTCGATAAAGGTGCCTCAGATTTGTTTTTCAGTGTCGGGGCACCGCCAAA
TATTAAAATCGAGGGCATCACATCACCTATTGGCCTGGTTCCTATCAAATCGAATCAAATGGCAGAAATTGTTTTATCTA
TTATGAATGACGCACAGCAAAAAGAATTTGAGTCTAAAATGGAACTCAATATGGCTTTTTCATTAGCTGGAGTAGGGCGT
TTCCGAATCAATTTATTCAGGCAACGTGGCGAAGTGGCTATGGTGGTGCGTTACATCAAGGGCGAAATACCTTCTATAGA
AGATTTAAATCTCCCTCAAATTTTAAAGTCTCTGGTCTTGGAGTTACGAGGTTTAGTATTGGTTGTTGGGTCCACGGGAT
CGGGTAAATCAACGACTCTGGCCGCGATGATCAACCACCGTAATGAAAATCACAGAGGGCATATTTTAACAATAGAGGAT
CCTATTGAATATATACATCAACATAAAAAATCAATAGTTGATCAACGAGAGATAGGCATTGATACTCTAAGCTATGACAA
TGCATTGAAAAACGCAATGAGAGAAGCACCTGATGTGATATTAATTGGTGAAATTCGTGATCTTAACTCAATGAAACATG
CGATAGCTTATGCTGAAACAGGACATTTGTGCATTTCTACTTTGCATGCCAATAACGCCAATCAGGCTCTGGATAGAGTG
ATTAATTTTTTCCCCGATGAGGCAAGACAACAGATATTATTAGATTTATCTCTTAATTTAAAAGCAATCATTTCATTACG
TTTGATACCAGGAGTTGATAATCAACGTGTCGCAGCGGTGGAGATCTTGATTAACTCTCCTTACATTTCCGATTTGATTG
AAAAAGGGAAGATAGACGATATTAAAGAAGTCATGGCAAGAAGCACGGAACAAGGCATGCAGACTTTCGATCAGGCTTTA
TTTAATTTATATAAAGAAGGAAAAATTACCAAAGAAAATGCGATTAAGTATGCTGATTCAAAAAACAATGTGGGTTTACA
AATTCGATTGGGCGAAGAAAAAAATTTTAATATTCAAGATGAGAATTTACAAATACAACCCGATGATATTGATAAGTTTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(42-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

55.056

95.442

0.525
  pilU Acinetobacter baylyi ADP1

52.941

95.71

0.507
  pilU Vibrio cholerae strain A1552

48.286

93.834

0.453
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.428

90.885

0.413
  pilT Legionella pneumophila strain ERS1305867

43.567

91.689

0.399
  pilT Legionella pneumophila strain Lp02

43.567

91.689

0.399
  pilT Neisseria meningitidis 8013

42.651

93.029

0.397
  pilT Neisseria gonorrhoeae MS11

42.651

93.029

0.397
  pilT Pseudomonas aeruginosa PAK

42.857

91.957

0.394
  pilT Pseudomonas stutzeri DSM 10701

42.274

91.957

0.389
  pilT Acinetobacter baumannii strain A118

43.413

89.544

0.389
  pilT Acinetobacter nosocomialis M2

43.413

89.544

0.389
  pilT Acinetobacter baumannii D1279779

43.413

89.544

0.389
  pilT Acinetobacter baylyi ADP1

41.176

91.153

0.375
  pilT Vibrio cholerae strain A1552

41.945

88.204

0.37
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.945

88.204

0.37

Multiple sequence alignment