Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DQM63_RS11195 Genome accession   NZ_LS483385
Coordinates   2230494..2231558 (-) Length   354 a.a.
NCBI ID   WP_002899359.1    Uniprot ID   A0A2X3V5M7
Organism   Streptococcus sanguinis strain NCTC7863     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2225494..2236558
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQM63_RS11170 (NCTC7863_02204) - 2226900..2227394 (-) 495 WP_002913919.1 hypothetical protein -
  DQM63_RS11175 (NCTC7863_02205) - 2227508..2227963 (-) 456 WP_002913918.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DQM63_RS11180 (NCTC7863_02206) - 2228036..2228494 (-) 459 WP_002913917.1 type II secretion system protein -
  DQM63_RS11185 (NCTC7863_02207) - 2228636..2229142 (-) 507 WP_002913916.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  DQM63_RS11190 (NCTC7863_02208) - 2229264..2230490 (-) 1227 WP_002913914.1 type II secretion system F family protein -
  DQM63_RS11195 (NCTC7863_02209) pilT 2230494..2231558 (-) 1065 WP_002899359.1 type IV pilus twitching motility protein PilT Machinery gene
  DQM63_RS11200 (NCTC7863_02210) - 2231560..2233242 (-) 1683 WP_002901897.1 GspE/PulE family protein -
  DQM63_RS11205 (NCTC7863_02211) - 2234365..2235252 (-) 888 WP_002913912.1 cation diffusion facilitator family transporter -
  DQM63_RS11210 (NCTC7863_02212) - 2235422..2235943 (+) 522 WP_002910979.1 TetR/AcrR family transcriptional regulator -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39285.37 Da        Isoelectric Point: 7.0216

>NTDB_id=1139662 DQM63_RS11195 WP_002899359.1 2230494..2231558(-) (pilT) [Streptococcus sanguinis strain NCTC7863]
MNLDELIKQAIEAGASDIHFTVGAEPTMRLHGRLTQLNNTILTNEDTVRFTKHILNEKQIAHLLEVGEVDCAYEVDNGYR
LRVNIFKQKNNCGIALRVIPKDIPSMESLGLPPVIKKLAEKRRGLILVTGPTGSGKSTTLATMINYMNSLRDEHIITIED
PIEYMHTHNKCLVNQRELGADTQSFGNALRGALRQDPDVILVGEMRDKETIEIALRAAETGHLVLSTLHTVGAVNTMDRI
IDVFPAEQQEQIRVQLSAVMEGVVSQQLMRTATGKGRVAAFEIMLGTPAIRNLIREGKTHQLLTPIQTGAQLGMITMDTS
LMGLYRRNVIDQRTLLSYSVDRAQVEKSLGIVGY

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1139662 DQM63_RS11195 WP_002899359.1 2230494..2231558(-) (pilT) [Streptococcus sanguinis strain NCTC7863]
ATGAATTTGGATGAGTTAATTAAGCAGGCGATTGAAGCGGGTGCATCAGATATCCACTTCACGGTTGGCGCTGAGCCAAC
CATGCGTCTGCACGGGAGGTTAACACAACTCAACAATACAATTTTGACCAACGAAGACACGGTTCGCTTCACCAAGCATA
TTTTAAATGAGAAGCAGATTGCTCATCTTTTAGAAGTCGGTGAAGTGGACTGTGCCTACGAGGTCGATAATGGCTATCGC
TTGCGGGTCAATATCTTTAAGCAGAAAAACAACTGCGGGATTGCCTTGCGGGTTATTCCCAAGGATATTCCATCTATGGA
ATCTCTGGGATTGCCACCAGTTATCAAAAAGCTAGCTGAGAAACGCCGGGGGCTGATATTGGTTACCGGGCCAACAGGAA
GCGGAAAGTCAACGACCCTGGCAACCATGATCAACTACATGAATAGCTTGCGGGACGAGCACATCATCACGATTGAAGAT
CCGATCGAGTACATGCATACGCATAATAAGTGCTTGGTCAACCAGCGTGAGCTAGGAGCAGATACACAAAGCTTCGGGAA
TGCCCTCCGCGGTGCACTTCGTCAGGACCCGGATGTCATCCTAGTCGGTGAGATGCGGGATAAGGAAACGATTGAAATCG
CCCTGCGTGCGGCGGAAACAGGTCACTTAGTCCTGTCAACTCTCCACACGGTCGGTGCCGTAAACACCATGGACCGGATT
ATCGACGTATTTCCAGCCGAGCAGCAGGAGCAAATCCGTGTACAGCTTTCTGCCGTTATGGAAGGGGTTGTATCGCAGCA
GCTCATGCGTACAGCGACCGGTAAAGGTCGTGTGGCTGCCTTTGAAATCATGCTGGGAACACCCGCTATTCGCAACCTCA
TTCGCGAAGGGAAGACCCACCAGCTGCTTACGCCAATCCAAACAGGGGCTCAGCTAGGCATGATTACTATGGATACTTCG
CTCATGGGACTCTACCGTCGCAATGTCATCGATCAAAGAACGCTACTGTCCTACTCAGTGGATCGCGCTCAAGTTGAAAA
ATCCCTAGGAATAGTGGGGTACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(6-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2X3V5M7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

51.497

94.35

0.486
  pilT Acinetobacter baumannii D1279779

51.497

94.35

0.486
  pilT Acinetobacter baumannii strain A118

51.497

94.35

0.486
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.445

95.198

0.48
  pilT Vibrio cholerae strain A1552

50.445

95.198

0.48
  pilT Legionella pneumophila strain Lp02

50.898

94.35

0.48
  pilT Legionella pneumophila strain ERS1305867

50.898

94.35

0.48
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.267

96.328

0.475
  pilT Pseudomonas aeruginosa PAK

50.301

93.785

0.472
  pilT Pseudomonas stutzeri DSM 10701

50

93.785

0.469
  pilT Acinetobacter baylyi ADP1

49.849

93.503

0.466
  pilT Neisseria meningitidis 8013

49.394

93.22

0.46
  pilT Neisseria gonorrhoeae MS11

49.091

93.22

0.458
  pilU Pseudomonas stutzeri DSM 10701

44.615

91.808

0.41
  pilU Vibrio cholerae strain A1552

41.108

96.893

0.398
  pilU Acinetobacter baylyi ADP1

41.159

92.655

0.381

Multiple sequence alignment