Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   DQN92_RS02555 Genome accession   NZ_LS483377
Coordinates   557336..558463 (-) Length   375 a.a.
NCBI ID   WP_004141526.1    Uniprot ID   A0A854E1G1
Organism   Stenotrophomonas maltophilia strain NCTC10258     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 552336..563463
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DQN92_RS02535 (NCTC10258_00510) - 552763..553752 (+) 990 WP_043033907.1 fumarylacetoacetate hydrolase family protein -
  DQN92_RS02540 (NCTC10258_00511) maiA 553767..554435 (+) 669 WP_033835418.1 maleylacetoacetate isomerase -
  DQN92_RS02545 (NCTC10258_00512) - 554481..555941 (-) 1461 WP_111686839.1 UdgX family uracil-DNA binding protein -
  DQN92_RS02550 (NCTC10258_00513) - 555922..557205 (-) 1284 WP_088431874.1 putative DNA modification/repair radical SAM protein -
  DQN92_RS02555 (NCTC10258_00514) pilU 557336..558463 (-) 1128 WP_004141526.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  DQN92_RS02560 (NCTC10258_00515) - 558585..558950 (+) 366 WP_005407961.1 DUF4398 domain-containing protein -
  DQN92_RS02565 (NCTC10258_00516) - 558947..559831 (+) 885 WP_080334765.1 OmpA family protein -
  DQN92_RS02570 (NCTC10258_00518) - 560156..560392 (+) 237 WP_005407964.1 YdcH family protein -
  DQN92_RS02575 (NCTC10258_00519) - 560693..562063 (+) 1371 WP_005407965.1 pyridoxal-phosphate dependent enzyme -
  DQN92_RS02580 (NCTC10258_00520) - 562187..563356 (+) 1170 WP_111686840.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 375 a.a.        Molecular weight: 41398.70 Da        Isoelectric Point: 6.3741

>NTDB_id=1139182 DQN92_RS02555 WP_004141526.1 557336..558463(-) (pilU) [Stenotrophomonas maltophilia strain NCTC10258]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKNKEGRRLPATEVLLNTPMIRDLLRRGQVHEIKAAMEASLEEGMESFDQCL
FRLAKAGTIEQEEALRAADSRDGLALKFRLSEGSSGEHDPYADFSAGAPSITHGF

Nucleotide


Download         Length: 1128 bp        

>NTDB_id=1139182 DQN92_RS02555 WP_004141526.1 557336..558463(-) (pilU) [Stenotrophomonas maltophilia strain NCTC10258]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAACGCTTCGGACATGTTTCTGACCACCGGGGCACCGGTCTA
CATCAAGATCGAGGGGAAGCTCTATCCGCTGGGCAACACCGGCCTGCCGCCGGGCATGGTCAAGAAGATCGCCTACTCGC
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAACTCAACATGGCCATCGCCCTGCCCGATGCCGGTCGC
TTCCGCGTCAATGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTCATCCGTGCCATCCGCAGCCGGATTCCGAGCATCGA
GGAGCTGAACCTGCCGCAGGTGCTGAAGGACGTCATCATGACCCCGCGCGGGCTGGTACTGGTGGTGGGGTCCACCGGTT
CCGGCAAGTCCACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGTCACATCCTCACCATCGAAGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTCAACCAGCGCGAGGTCGGGCTGGATACCCATGCCTTCCACAA
CGCGCTGAAGAACGCCATGCGTGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCAGAGACGATGGAAGCGG
CGATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACCCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTGAACTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGTGCGGTGATCAGCCAGCG
CCTGGTGAAGAACAAGGAAGGCCGTCGCCTGCCGGCCACCGAAGTGCTGCTCAACACGCCGATGATCCGCGACCTGCTGC
GTCGCGGCCAGGTGCACGAAATCAAGGCGGCGATGGAAGCATCGCTGGAAGAGGGCATGGAGAGTTTCGACCAGTGCCTG
TTCCGACTAGCCAAGGCCGGCACCATCGAGCAGGAGGAGGCGCTGCGCGCCGCCGACTCGCGCGATGGCCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCAGCAGCGGCGAGCATGACCCGTACGCGGATTTCTCGGCCGGCGCACCCAGCATCACCCACG
GCTTCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A854E1G1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.975

95.733

0.488
  pilU Vibrio cholerae strain A1552

49.435

94.4

0.467
  pilU Acinetobacter baylyi ADP1

49.153

94.4

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

45.04

99.467

0.448
  pilT Pseudomonas aeruginosa PAK

42.604

90.133

0.384
  pilT Acinetobacter baumannii strain A118

41.42

90.133

0.373
  pilT Acinetobacter nosocomialis M2

41.42

90.133

0.373
  pilT Acinetobacter baumannii D1279779

41.42

90.133

0.373
  pilT Acinetobacter baylyi ADP1

41.176

90.667

0.373
  pilT Pseudomonas stutzeri DSM 10701

41.124

90.133

0.371
  pilT Legionella pneumophila strain Lp02

40.533

90.133

0.365
  pilT Legionella pneumophila strain ERS1305867

40.533

90.133

0.365
  pilT Vibrio cholerae strain A1552

41.141

88.8

0.365
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.141

88.8

0.365
  pilT Neisseria gonorrhoeae MS11

38.905

92.533

0.36
  pilT Neisseria meningitidis 8013

38.905

92.533

0.36

Multiple sequence alignment