Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   MORIYA_RS16145 Genome accession   NZ_LS483250
Coordinates   3479947..3481062 (+) Length   371 a.a.
NCBI ID   WP_112716795.1    Uniprot ID   A0A330LT26
Organism   Moritella yayanosii strain DB21MT 5     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3474947..3486062
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MORIYA_RS16115 (MORIYA_3388) - 3475273..3475887 (-) 615 WP_112716783.1 XTP/dITP diphosphatase -
  MORIYA_RS16120 (MORIYA_3389) - 3475986..3476414 (-) 429 WP_112716785.1 DUF4426 domain-containing protein -
  MORIYA_RS16125 (MORIYA_3390) - 3476693..3477238 (-) 546 WP_112716787.1 YggT family protein -
  MORIYA_RS16130 (MORIYA_3391) proC 3477291..3478118 (-) 828 WP_112716789.1 pyrroline-5-carboxylate reductase -
  MORIYA_RS16135 (MORIYA_3392) - 3478175..3478870 (-) 696 WP_112716791.1 YggS family pyridoxal phosphate-dependent enzyme -
  MORIYA_RS16140 (MORIYA_3393) pilT 3478901..3479935 (+) 1035 WP_112716793.1 type IV pilus twitching motility protein PilT Machinery gene
  MORIYA_RS16145 (MORIYA_3394) pilU 3479947..3481062 (+) 1116 WP_112716795.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  MORIYA_RS16150 (MORIYA_3395) yaaA 3481101..3481880 (+) 780 WP_112716797.1 peroxide stress protein YaaA -
  MORIYA_RS16155 (MORIYA_3396) - 3482047..3482694 (-) 648 WP_112716799.1 TIGR04211 family SH3 domain-containing protein -
  MORIYA_RS16160 (MORIYA_3397) - 3482857..3484131 (-) 1275 WP_112716801.1 inorganic phosphate transporter -
  MORIYA_RS16165 (MORIYA_3398) - 3484154..3484834 (-) 681 WP_112716803.1 TIGR00153 family protein -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 40793.01 Da        Isoelectric Point: 6.2689

>NTDB_id=1135272 MORIYA_RS16145 WP_112716795.1 3479947..3481062(+) (pilU) [Moritella yayanosii strain DB21MT 5]
MGMLPQLFSAMKKLAGSDMYISAGIAPTVKVHGSLRAISEHKLTAEQSLALVKEAMSEDEYASFCRTKESNFGIFMPDIG
RFRVSAFWQLEKAGMVIRRIETEIPTMDELHLPPILKHTALEKRGLILVVGATGSGKSTTQAAMVGYRNQNSSGHILTIE
DPIEFVHEHGSCIVTQREVGIDTESFENALKSSLRQAPDVILIGEIRSQETMEFALAFAETGHLCMATLHATNANQAIDR
ILHLVPKEKHAQLLYDLSLNLKAIVAQQLIPVQGGTSRRGAFEILLNTPLISELIRNNELHKIKDVMTKSVELGMQTFDQ
ALLALYNSSMISYTEALHHADSANDLRLMIKLNQGQGSSDGMLDGVTIEAL

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=1135272 MORIYA_RS16145 WP_112716795.1 3479947..3481062(+) (pilU) [Moritella yayanosii strain DB21MT 5]
ATGGGGATGCTACCGCAACTCTTTAGTGCCATGAAAAAGCTTGCCGGTTCTGATATGTATATCTCTGCGGGGATTGCGCC
GACAGTTAAAGTTCACGGGTCATTACGTGCAATCTCGGAACATAAATTAACCGCAGAGCAATCGTTAGCCTTAGTCAAAG
AAGCCATGTCTGAGGATGAGTATGCTTCTTTTTGTCGTACCAAAGAATCTAATTTTGGTATTTTTATGCCAGATATTGGT
CGTTTCCGTGTCAGTGCATTTTGGCAATTAGAAAAAGCTGGTATGGTTATTCGTCGTATTGAAACCGAAATCCCGACCAT
GGATGAATTACACCTGCCACCAATATTAAAACACACGGCACTCGAGAAGCGTGGTTTAATATTGGTGGTCGGCGCGACGG
GTTCGGGCAAATCCACCACCCAAGCAGCGATGGTGGGTTATCGTAATCAAAACTCATCGGGGCATATCTTAACCATTGAA
GATCCGATTGAGTTTGTGCATGAACATGGCAGTTGTATTGTCACCCAGCGTGAAGTGGGGATTGATACCGAGTCGTTTGA
AAATGCGTTAAAGAGTTCCTTGCGCCAAGCGCCTGATGTCATTCTTATTGGAGAGATCCGCTCGCAAGAAACCATGGAAT
TTGCCTTGGCGTTTGCGGAAACTGGTCACTTATGTATGGCGACGTTACATGCCACCAATGCTAATCAAGCCATTGATCGT
ATTTTGCACTTAGTACCAAAAGAAAAACATGCGCAATTGTTATATGATTTGTCGCTGAATTTAAAAGCCATTGTTGCGCA
GCAATTAATTCCCGTGCAGGGAGGGACCAGTCGACGAGGAGCATTCGAGATCTTATTGAATACGCCATTGATCAGTGAGT
TGATCCGTAACAATGAGCTGCATAAAATTAAAGATGTAATGACGAAATCGGTAGAATTGGGTATGCAAACCTTTGACCAA
GCGTTATTAGCGCTTTATAACAGTAGTATGATCAGTTATACCGAAGCCCTGCATCATGCTGATTCAGCCAATGATTTACG
CTTAATGATTAAGTTAAATCAAGGTCAAGGGAGCAGTGATGGGATGTTGGATGGAGTCACGATTGAAGCCTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A330LT26

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

60.383

98.652

0.596
  pilU Pseudomonas stutzeri DSM 10701

59.366

93.531

0.555
  pilU Acinetobacter baylyi ADP1

57.88

94.07

0.544
  pilT Pseudomonas aeruginosa PAK

43.544

89.757

0.391
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.21

87.332

0.377
  pilT Pseudomonas stutzeri DSM 10701

42.042

89.757

0.377
  pilT Legionella pneumophila strain ERS1305867

41.945

88.679

0.372
  pilT Legionella pneumophila strain Lp02

41.945

88.679

0.372
  pilT Vibrio cholerae strain A1552

43.831

83.019

0.364
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

43.831

83.019

0.364
  pilT Acinetobacter nosocomialis M2

41.033

88.679

0.364
  pilT Acinetobacter baumannii D1279779

41.033

88.679

0.364
  pilT Acinetobacter baumannii strain A118

41.033

88.679

0.364
  pilT Neisseria gonorrhoeae MS11

39.528

91.375

0.361
  pilT Neisseria meningitidis 8013

39.528

91.375

0.361

Multiple sequence alignment