Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ETECE925_RS04325 Genome accession   NZ_LR883000
Coordinates   878667..879647 (+) Length   326 a.a.
NCBI ID   WP_088535850.1    Uniprot ID   A0A7I8YX55
Organism   Escherichia coli isolate L6_E562_ETEC     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 873667..884647
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ETECE925_RS04295 (ETECE562_00967) yggM 874182..875189 (+) 1008 WP_000745210.1 DUF1202 family protein -
  ETECE925_RS04300 (ETECE562_00968) hemW 875344..876480 (-) 1137 WP_000239918.1 radical SAM family heme chaperone HemW -
  ETECE925_RS04305 (ETECE562_00969) rdgB 876473..877066 (-) 594 WP_001174747.1 XTP/dITP diphosphatase -
  ETECE925_RS04310 (ETECE562_00970) yggU 877074..877364 (-) 291 WP_000994920.1 DUF167 family protein YggU -
  ETECE925_RS04315 (ETECE562_00971) yggT 877361..877927 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  ETECE925_RS04320 (ETECE562_00972) yggS 877945..878649 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  ETECE925_RS04325 (ETECE562_00973) pilT 878667..879647 (+) 981 WP_088535850.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ETECE925_RS04330 - 879696..879824 (+) 129 WP_001278270.1 hypothetical protein -
  ETECE925_RS04335 (ETECE562_00974) ruvX 879831..880247 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  ETECE925_RS04340 (ETECE562_00975) yqgE 880247..880810 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  ETECE925_RS04345 (ETECE562_00976) gshB 880919..881869 (-) 951 WP_000593273.1 glutathione synthase -
  ETECE925_RS04350 (ETECE562_00977) rsmE 881882..882613 (-) 732 WP_001222508.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ETECE925_RS04355 (ETECE562_00978) endA 882693..883400 (-) 708 WP_000286511.1 deoxyribonuclease I -
  ETECE925_RS04360 (ETECE562_00979) yggI 883495..883992 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35912.11 Da        Isoelectric Point: 5.7654

>NTDB_id=1133065 ETECE925_RS04325 WP_088535850.1 878667..879647(+) (pilT) [Escherichia coli isolate L6_E562_ETEC]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGMILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGVRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQHR
VGEGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1133065 ETECE925_RS04325 WP_088535850.1 878667..879647(+) (pilT) [Escherichia coli isolate L6_E562_ETEC]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGGCTAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCATGATTCTGGTGACGGGGGCGACGGGGAGTGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGGGTGCG
GGCCGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTACGTGACAGTGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACCTTACATACACGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGACCCCGTGCGTAATCAACTGGCAGGTAGTTTACGGGCAGTGCTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTATTTGAATTGCTGATTAACACACCCGCGGTGGGGAATTTGATTCGTGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCACCGG
GTGGGGGAAGGGCGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7I8YX55

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

48.78

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.476

100

0.488
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.488
  pilT Vibrio cholerae strain A1552

48.624

100

0.488
  pilT Acinetobacter baumannii D1279779

46.177

100

0.463
  pilT Acinetobacter nosocomialis M2

46.177

100

0.463
  pilT Acinetobacter baumannii strain A118

46.177

100

0.463
  pilT Pseudomonas stutzeri DSM 10701

45.872

100

0.46
  pilT Acinetobacter baylyi ADP1

45.566

100

0.457
  pilT Pseudomonas aeruginosa PAK

45.26

100

0.454
  pilT Legionella pneumophila strain ERS1305867

45.597

97.546

0.445
  pilT Legionella pneumophila strain Lp02

45.597

97.546

0.445
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.509

97.546

0.405
  pilU Vibrio cholerae strain A1552

39.752

98.773

0.393
  pilU Pseudomonas stutzeri DSM 10701

36.97

100

0.374
  pilU Acinetobacter baylyi ADP1

36.747

100

0.374

Multiple sequence alignment