Detailed information NTG1132380

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: CFBP498_RS05560
• Genome accession: NZ_LR828253
• Coordinates: 1321735..1322151 (+)
• Length: 138 a.a.
• NCBI ID: WP_006448362.1
• Uniprot ID: A0A6V7CCW8
• Organism: Xanthomonas hortorum pv. gardneri strain CFBP 8129
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1316735..1327151

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CFBP498_RS05530 (CFBP8129_11180)	-	1317104..1317406 (-)	303	WP_006448357.1	type II toxin-antitoxin system RelE/ParE family toxin	-
CFBP498_RS05535 (CFBP8129_11190)	-	1317403..1317588 (-)	186	WP_006448358.1	hypothetical protein	-
CFBP498_RS05540 (CFBP8129_11200)	-	1317686..1318087 (-)	402	WP_006448359.1	SymE family type I addiction module toxin	-
CFBP498_RS05545 (CFBP8129_11220)	coaE	1318621..1319244 (-)	624	WP_006448360.1	dephospho-CoA kinase	-
CFBP498_RS05550 (CFBP8129_11230)	-	1319258..1320121 (-)	864	WP_002812278.1	prepilin peptidase	-
CFBP498_RS05555 (CFBP8129_11240)	pilC	1320128..1321384 (-)	1257	WP_043907071.1	type II secretion system F family protein	Machinery gene
CFBP498_RS05560 (CFBP8129_11250)	pilA	1321735..1322151 (+)	417	WP_006448362.1	pilin	Machinery gene
CFBP498_RS05565	-	1322228..1322659 (+)	432	WP_043907066.1	pilin	-
CFBP498_RS05570 (CFBP8129_11260)	pilB	1322724..1324460 (+)	1737	WP_043907067.1	type IV-A pilus assembly ATPase PilB	Machinery gene
CFBP498_RS05575 (CFBP8129_11270)	-	1324590..1324772 (-)	183	WP_074066024.1	YhfG family protein	-
CFBP498_RS05580 (CFBP8129_11280)	pilR	1324846..1326240 (-)	1395	WP_006448366.1	sigma-54-dependent transcriptional regulator	Regulator

Sequence

Protein

• Length: 138 a.a.
• Molecular weight: 14334.62 Da
• Isoelectric Point: 8.4815

>NTDB_id=1132380 CFBP498_RS05560 WP_006448362.1 1321735..1322151(+) (pilA) [Xanthomonas hortorum pv. gardneri strain CFBP 8129]
MKKQQGFTLIELMIVVAIIAILAAIALPAYQNYVAKSQVAAALAEITPGKTQYEVMVNEGKAASITPDLIGLKTSNRCTI
STPNPGETGAITCALLNSSPKVGAASISWTRAADGTWTCSTTLATADKLKYAPSTCQG

Nucleotide

• Length: 417 bp

>NTDB_id=1132380 CFBP498_RS05560 WP_006448362.1 1321735..1322151(+) (pilA) [Xanthomonas hortorum pv. gardneri strain CFBP 8129]
ATGAAAAAGCAGCAAGGTTTTACGTTAATTGAATTGATGATCGTGGTTGCCATCATTGCCATCTTGGCAGCGATTGCTCT
GCCGGCGTATCAAAACTATGTGGCCAAATCGCAAGTTGCTGCAGCCTTGGCTGAGATCACTCCTGGCAAGACTCAGTACG
AAGTGATGGTTAACGAAGGCAAGGCGGCGTCGATTACTCCGGATCTCATCGGTCTTAAGACATCCAACCGTTGCACGATC
AGCACCCCGAATCCGGGTGAGACTGGCGCTATCACGTGTGCCTTGTTGAATTCCAGTCCGAAGGTTGGCGCCGCTTCTAT
CTCCTGGACCCGAGCTGCGGATGGTACGTGGACCTGTTCGACCACGTTGGCCACTGCTGATAAGCTTAAGTATGCTCCTT
CAACCTGCCAGGGTTAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (1-27)

• Pilin (bacterial filament) (Pilin) (35-137)

3D structure

• AlphaFold DB: A0A6V7CCW8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Acinetobacter baumannii strain A118	47.887	100	0.493
pilA/pilAI	Pseudomonas stutzeri DSM 10701	49.275	100	0.493
pilA/pilAII	Pseudomonas stutzeri DSM 10701	47.445	99.275	0.471
pilA	Pseudomonas aeruginosa PAK	43.796	99.275	0.435
comP	Acinetobacter baylyi ADP1	39.735	100	0.435
pilA	Vibrio cholerae C6706	39.31	100	0.413
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	39.31	100	0.413
pilA	Vibrio cholerae strain A1552	39.31	100	0.413
pilA/pilA1	Eikenella corrodens VA1	34.839	100	0.391
pilA	Vibrio parahaemolyticus RIMD 2210633	40.909	95.652	0.391
pilA2	Legionella pneumophila strain ERS1305867	36.986	100	0.391
pilA2	Legionella pneumophila str. Paris	37.241	100	0.391
pilE	Neisseria gonorrhoeae MS11	33.333	100	0.384