Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CFBP498_RS05570 Genome accession   NZ_LR828253
Coordinates   1322724..1324460 (+) Length   578 a.a.
NCBI ID   WP_043907067.1    Uniprot ID   -
Organism   Xanthomonas hortorum pv. gardneri strain CFBP 8129     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1317724..1329460
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFBP498_RS05545 (CFBP8129_11220) coaE 1318621..1319244 (-) 624 WP_006448360.1 dephospho-CoA kinase -
  CFBP498_RS05550 (CFBP8129_11230) - 1319258..1320121 (-) 864 WP_002812278.1 prepilin peptidase -
  CFBP498_RS05555 (CFBP8129_11240) pilC 1320128..1321384 (-) 1257 WP_043907071.1 type II secretion system F family protein Machinery gene
  CFBP498_RS05560 (CFBP8129_11250) pilA 1321735..1322151 (+) 417 WP_006448362.1 pilin Machinery gene
  CFBP498_RS05565 - 1322228..1322659 (+) 432 WP_043907066.1 pilin -
  CFBP498_RS05570 (CFBP8129_11260) pilB 1322724..1324460 (+) 1737 WP_043907067.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CFBP498_RS05575 (CFBP8129_11270) - 1324590..1324772 (-) 183 WP_074066024.1 YhfG family protein -
  CFBP498_RS05580 (CFBP8129_11280) pilR 1324846..1326240 (-) 1395 WP_006448366.1 sigma-54-dependent transcriptional regulator Regulator
  CFBP498_RS05585 (CFBP8129_11290) - 1326386..1327999 (-) 1614 WP_006448368.1 sensor histidine kinase -
  CFBP498_RS05590 (CFBP8129_11300) sucC 1328232..1329401 (+) 1170 WP_006450728.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62675.84 Da        Isoelectric Point: 5.6039

>NTDB_id=1132381 CFBP498_RS05570 WP_043907067.1 1322724..1324460(+) (pilB) [Xanthomonas hortorum pv. gardneri strain CFBP 8129]
MNVSASANLVGITGIARRLVQDGALEESVARSAMDQAASAKVPLPQWFAEKKLVSAAQLAAANAVEFGMPLMDVSVFDAS
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGASLG
NDDDDMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQK
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKAPLP
EHALLAEGFTPEQVASGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQRIGIRDLRQSALV
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=1132381 CFBP498_RS05570 WP_043907067.1 1322724..1324460(+) (pilB) [Xanthomonas hortorum pv. gardneri strain CFBP 8129]
ATGAACGTGAGTGCATCTGCAAATTTAGTTGGTATTACCGGCATTGCGCGTCGTCTAGTCCAAGATGGTGCGCTTGAAGA
GTCTGTTGCTCGGTCTGCGATGGATCAAGCTGCGTCGGCGAAAGTTCCATTGCCACAGTGGTTTGCTGAGAAAAAGCTAG
TTTCAGCCGCTCAGCTGGCTGCAGCTAATGCAGTCGAGTTCGGCATGCCGTTGATGGATGTGTCGGTCTTCGACGCCAGC
CAGAACGCCGTCAAGCTGGTCAGCGAGGAATTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAATCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCATACCAACTTGGTGGTGGAGCCGA
TTCTGGTCGATGAGGACCAGATCCGGCGCACCCTGGAGCAGTGGCAGGCCAGTAATGCCGCACTGGGCGCGTCGCTCGGC
AACGACGACGACGATATGGGGGACCTGGACGTGTCGGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCTGTGGTGAAGTTCGTCAACAAAGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCGGACA
TTCATTTCGAGCCGTACGAAGACGACTACCGGGTACGCTTGCGCATCGATGGCCTGCTGAAGAATGTGGCCAAGGCGCCG
GTGAAGTTGAGCCAGCGCATCGCGGCGCGCCTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGTATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGTACCTTGCCGACCCTGTTCGGCGAGA
AGGTTGTGCTGCGTATCCTGGACGCCAGTGCGGCCAAGCTGGGGATCGAGAAGCTGGGCTACGAGGCGGATCAGCAGAAG
CTGTTCCTGGATGCGATTCACAAGCCCTACGGGATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTGGGCATCCTCAACGATGAGACGCGCAATATCTCCACGGCCGAGGATCCGGTCGAAATCCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACGTCAAGCGCGGCATGACGTTTGCTGCGGCGTTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATTGCAATCAAGGCGGCGCAGACCGGGCACATGGT
GTTGTCGACGCTGCACACCAACGATGCGCCGCAGACCATCGCGCGTCTGATGAACATGGGCATTGCGCCGTACAACATCA
CCTCGTCGGTGACCTTGGTCATCGCGCAGCGTCTGGCGCGGCGTTTGTGCAACAACTGCAAGCGCAAGGCACCGCTGCCG
GAGCACGCGCTGTTGGCCGAAGGCTTCACGCCAGAGCAGGTCGCTTCCGGCATCGAGCTATACGAAGCGGTTGGTTGCGA
TGAGTGCACAGAAGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGGTGCGATCGTGC
TTGAAGGCGGTAACGCGATGCAGATCGCCGAGGCGGCGCAGAGGATCGGCATCCGCGATTTGCGGCAGTCGGCGCTGGTG
AAGGCGGCGCATGGGGTGACGAGCTTGGCTGAGATCAATCGGGTGACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(201-468)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.939

99.827

0.548
  pilB Acinetobacter baumannii D1279779

55.458

98.27

0.545
  pilB Legionella pneumophila strain ERS1305867

52.021

98.443

0.512
  pilF Neisseria gonorrhoeae MS11

48.239

98.27

0.474
  pilB Vibrio cholerae strain A1552

49.455

95.156

0.471
  pilB Vibrio campbellii strain DS40M4

45.87

98.443

0.452
  pilB Vibrio parahaemolyticus RIMD 2210633

46.429

96.886

0.45
  pilF Thermus thermophilus HB27

40.115

90.138

0.362

Multiple sequence alignment