Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   KR49_RS08245 Genome accession   NZ_CP006270
Coordinates   1633541..1634617 (-) Length   358 a.a.
NCBI ID   WP_043693972.1    Uniprot ID   A0A076HA21
Organism   Synechococcus sp. KORDI-49 strain KORDI 49     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1628541..1639617
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KR49_RS08220 (KR49_08980) - 1628840..1629475 (+) 636 WP_043693963.1 RNA-binding protein -
  KR49_RS08225 (KR49_08985) dusA 1629483..1630487 (-) 1005 WP_043693966.1 tRNA dihydrouridine(20/20a) synthase DusA -
  KR49_RS08230 (KR49_08990) msrB 1630553..1631056 (+) 504 WP_043697154.1 peptide-methionine (R)-S-oxide reductase MsrB -
  KR49_RS08235 (KR49_08995) - 1631091..1632296 (+) 1206 WP_253912739.1 NAD(P)/FAD-dependent oxidoreductase -
  KR49_RS08240 (KR49_09000) - 1632271..1633521 (-) 1251 WP_043693969.1 type II secretion system F family protein -
  KR49_RS08245 (KR49_09005) pilT 1633541..1634617 (-) 1077 WP_043693972.1 type IV pilus twitching motility protein PilT Machinery gene
  KR49_RS08250 (KR49_09010) - 1634628..1636454 (-) 1827 WP_371257623.1 GspE/PulE family protein -
  KR49_RS08255 (KR49_09015) grpE 1636552..1637232 (+) 681 WP_043693977.1 nucleotide exchange factor GrpE -
  KR49_RS08260 (KR49_09020) dnaJ 1637232..1638362 (+) 1131 WP_043693980.1 molecular chaperone DnaJ -
  KR49_RS08265 (KR49_09025) - 1638359..1638586 (+) 228 WP_043693985.1 sulfurtransferase TusA family protein -
  KR49_RS08270 (KR49_09030) rsgA 1638593..1639492 (+) 900 WP_043693987.1 ribosome small subunit-dependent GTPase A -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39184.78 Da        Isoelectric Point: 5.4520

>NTDB_id=113079 KR49_RS08245 WP_043693972.1 1633541..1634617(-) (pilT) [Synechococcus sp. KORDI-49 strain KORDI 49]
MNLMIEDLMVQLVEAGGSDLHIATGQPPYGRFSGELRPMLEEPLEEEDCNRLIFSMLNNSQRKTLEQTWELDCAYGLRGV
ARFRVNVYRQKGSYAACLRALGSKIPSIEVLNLPPVVIETSKRPRGLVLVTGPTGSGKTTTLAALLDHINHTRSEHILTI
EDPIEFVYQSDKSLVHQRQLNEDTRSFANALRAALREDPDVILVGEMRDLETIQLAISAAETGHLVFGTLHTSSAAQTVD
RMVDVFPPGQQTQIRVQLSGSLVAVFSQTLCRRQNPAPGQFGRVMAQEIMINTPATANLIREGKTAQLYSQIQTGGELGM
QTLEKALANLVAAGEISMAEATAKASKPAELERLVGDP

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=113079 KR49_RS08245 WP_043693972.1 1633541..1634617(-) (pilT) [Synechococcus sp. KORDI-49 strain KORDI 49]
ATGAATCTGATGATCGAAGACCTGATGGTGCAGCTGGTCGAAGCAGGCGGCAGCGATCTGCACATCGCCACCGGACAGCC
TCCCTACGGACGTTTCAGCGGCGAACTGCGCCCGATGCTGGAGGAACCCCTGGAAGAGGAGGACTGCAACAGGCTGATCT
TCTCGATGCTGAACAACAGCCAGCGCAAGACCCTCGAACAGACCTGGGAACTGGACTGCGCCTACGGACTCAGAGGCGTC
GCCCGTTTCCGGGTGAACGTGTACCGGCAGAAGGGCAGTTATGCGGCCTGCCTGAGGGCCCTGGGAAGCAAGATCCCCAG
CATCGAGGTTCTGAACCTGCCTCCGGTCGTGATCGAGACCAGCAAGCGTCCGCGGGGCCTGGTGCTGGTCACGGGACCCA
CAGGTTCCGGCAAGACCACCACCCTGGCTGCACTGCTGGATCACATCAATCACACCCGCAGCGAACACATCCTCACCATC
GAGGATCCGATCGAATTCGTCTACCAGAGCGATAAGAGCCTGGTGCACCAACGCCAACTCAACGAAGACACCAGGAGCTT
CGCCAATGCCCTGCGTGCGGCATTGCGGGAAGACCCAGATGTGATCCTCGTCGGCGAAATGCGAGACCTGGAAACAATCC
AACTGGCCATCAGCGCTGCTGAAACCGGTCATCTTGTGTTCGGCACGCTGCACACCAGTTCGGCTGCCCAGACAGTGGAT
CGCATGGTGGATGTGTTCCCGCCAGGCCAACAGACCCAGATCCGTGTGCAGCTCTCGGGAAGTCTCGTGGCCGTGTTCTC
CCAGACCCTCTGCCGCCGTCAGAATCCGGCTCCCGGTCAGTTCGGTCGGGTGATGGCACAGGAAATCATGATCAACACCC
CGGCGACAGCCAACCTGATCCGGGAAGGGAAGACTGCTCAGCTCTATTCCCAGATCCAAACCGGTGGGGAACTGGGCATG
CAGACGCTTGAAAAAGCGCTGGCGAACCTGGTGGCAGCAGGTGAAATCAGCATGGCTGAGGCCACTGCCAAAGCCAGCAA
GCCGGCGGAGCTGGAACGTCTGGTCGGTGATCCCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(122-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A076HA21

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

54.942

96.089

0.528
  pilT Pseudomonas stutzeri DSM 10701

53.779

96.089

0.517
  pilT Acinetobacter baumannii D1279779

52.907

96.089

0.508
  pilT Acinetobacter baumannii strain A118

52.907

96.089

0.508
  pilT Acinetobacter nosocomialis M2

52.616

96.089

0.506
  pilT Legionella pneumophila strain Lp02

53.754

93.017

0.5
  pilT Legionella pneumophila strain ERS1305867

53.754

93.017

0.5
  pilT Acinetobacter baylyi ADP1

51.163

96.089

0.492
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.858

98.603

0.492
  pilT Neisseria gonorrhoeae MS11

50.437

95.81

0.483
  pilT Neisseria meningitidis 8013

51.343

93.575

0.48
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.294

94.972

0.478
  pilT Vibrio cholerae strain A1552

50.294

94.972

0.478
  pilU Pseudomonas stutzeri DSM 10701

39.886

98.045

0.391
  pilU Vibrio cholerae strain A1552

39.403

93.575

0.369
  pilU Acinetobacter baylyi ADP1

38.15

96.648

0.369

Multiple sequence alignment