Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   H1C96_RS18750 Genome accession   NZ_LR778149
Coordinates   3902834..3903814 (+) Length   326 a.a.
NCBI ID   WP_180344492.1    Uniprot ID   -
Organism   Escherichia coli isolate SC487     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3897834..3908814
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H1C96_RS18720 yggM 3898349..3899356 (+) 1008 WP_000745217.1 DUF1202 family protein -
  H1C96_RS18725 hemW 3899511..3900647 (-) 1137 WP_000239939.1 radical SAM family heme chaperone HemW -
  H1C96_RS18730 rdgB 3900640..3901233 (-) 594 WP_001174743.1 XTP/dITP diphosphatase -
  H1C96_RS18735 yggU 3901241..3901531 (-) 291 WP_001277222.1 DUF167 family protein YggU -
  H1C96_RS18740 yggT 3901528..3902094 (-) 567 WP_001094831.1 osmotic shock tolerance protein YggT -
  H1C96_RS18745 yggS 3902112..3902816 (-) 705 WP_000997795.1 pyridoxal phosphate homeostasis protein -
  H1C96_RS18750 pilT 3902834..3903814 (+) 981 WP_180344492.1 type IV pilus twitching motility protein PilT Machinery gene
  H1C96_RS18755 ruvX 3903990..3904406 (-) 417 WP_000017106.1 Holliday junction resolvase RuvX -
  H1C96_RS18760 yqgE 3904406..3904969 (-) 564 WP_001053178.1 YqgE/AlgH family protein -
  H1C96_RS18765 gshB 3905078..3906028 (-) 951 WP_000593273.1 glutathione synthase -
  H1C96_RS18770 rsmE 3906041..3906772 (-) 732 WP_001222508.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  H1C96_RS18775 endA 3906852..3907559 (-) 708 WP_000286489.1 deoxyribonuclease I -
  H1C96_RS18780 yggI 3907654..3908151 (-) 498 WP_000858396.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35889.07 Da        Isoelectric Point: 5.5857

>NTDB_id=1130127 H1C96_RS18750 WP_180344492.1 3902834..3903814(+) (pilT) [Escherichia coli isolate SC487]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQR
VGEGCL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1130127 H1C96_RS18750 WP_180344492.1 3902834..3903814(+) (pilT) [Escherichia coli isolate SC487]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGCATTCGCGGCAGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAAAATGGCCAGCTGGATTTTGCCGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCCTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTTAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTGGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACTTTCGCATCGGGGTTGCG
GGCTGCATTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCACTGACGG
CGGCAGAAACCGGGCATTTGGTGCTGGCAACATTACATACGCGTGGTGCCGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTACGTAATCAACTGGCAGGTAGTTTACGGGCCGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTATTGATTAACACTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTACCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGATAACGTTTCAGCAGAGTTATCAGCAGCGG
GTGGGGGAAGGGTGTTTGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(62-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

49.24

100

0.497
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.24

100

0.497
  pilT Neisseria meningitidis 8013

48.485

100

0.491
  pilT Neisseria gonorrhoeae MS11

48.182

100

0.488
  pilT Acinetobacter baylyi ADP1

46.505

100

0.469
  pilT Acinetobacter baumannii strain A118

46.201

100

0.466
  pilT Acinetobacter baumannii D1279779

46.201

100

0.466
  pilT Acinetobacter nosocomialis M2

46.201

100

0.466
  pilT Pseudomonas stutzeri DSM 10701

46.201

100

0.466
  pilT Pseudomonas aeruginosa PAK

45.897

100

0.463
  pilT Legionella pneumophila strain ERS1305867

44.985

100

0.454
  pilT Legionella pneumophila strain Lp02

44.985

100

0.454
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.138

97.546

0.411
  pilU Vibrio cholerae strain A1552

40.123

99.387

0.399
  pilU Pseudomonas stutzeri DSM 10701

38.438

98.16

0.377
  pilU Acinetobacter baylyi ADP1

36.957

98.773

0.365

Multiple sequence alignment